LOCUS       V040791                10594 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040791
VERSION     V040791
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4620
                     /label="Cul3(NM_016716)"
                     /note="Cul3(NM_016716)"
                     /gene="Cul3"
     CDS             4627..4692
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4736..4947
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4960..5228
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5257..5266
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5287..5949
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6019..6072
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6073..6672
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6673..7261
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7335..7568
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7640..7761
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7801..7936
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7969..7985)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7993..8009
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8017..8047)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8062..8083
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8371..8959)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9130..9990)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9991..10095)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10569..10585
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cgaatctgag caaaggcacg
     2341 ggcagccgga aggacaccaa gatgcggatc cgggcctttc cgatgaccat ggatgaaaaa
     2401 tatgtaaaca gcatttggga ccttctgaaa aatgcaattc aagaaatcca gcgtaagaat
     2461 aacagtggtc ttagttttga ggagctctat agaaatgcat atacaatggt tttgcataaa
     2521 catggagaaa agctctacac tggactaaga gaagttgtta ctgaacatct cataaataag
     2581 gtgcgagaag atgtactaaa ttcattgaat aacaactttc ttcaaacact aaatcaagct
     2641 tggaatgatc atcaaacagc catggtgatg attagagaca tattaatgta tatggaccgt
     2701 gtatatgtac aacagaataa tgtagaaaat gtctacaatt tgggattaat aatttttcga
     2761 gatcaagttg tacggtatgg gtgtattagg gatcatcttc ggcaaacttt attggatatg
     2821 attgccagag agcggaaagg agaagttgta gacagaggag caataagaaa tgcttgccag
     2881 atgttaatga ttttaggtct tgaaggaaga tcagtctatg aggaagattt tgaggctccg
     2941 tttttggaaa tgtctgcaga attttttcag atggaaagcc agaaattttt agcagaaaac
     3001 agtgcttcag tatatataaa gaaagtagaa gctaggatta atgaggaaat agagcgggtg
     3061 atgcactgcc ttgacaaatc cacagaagag cctattgtga aggtggtgga gagggaactc
     3121 atttccaagc acatgaagac tattgtagaa atggaaaatt ctgggctagt acatatgctg
     3181 aaaaatggaa agacagaaga ccttgcttgc atgtacaaat tatttagtcg tgtgccaaat
     3241 ggtttgaaga ccatgtgtga atgtatgagt tgttatttga gggaacaagg aaaagccctt
     3301 gtttctgagg aaggagaagg gaagaatcct gttgactata tccagggctt attggatcta
     3361 aagagtaggt ttgatcgctt cctccaagaa tccttcaata atgaccggct ctttaaacag
     3421 actattgctg gggacttcga gtatttctta aacctcaact ctaggtctcc tgaatacctc
     3481 tcattattta ttgatgataa actgaaaaag ggagtcaaag ggctaacaga acaagaagta
     3541 gaaacaatat tggataaggc aatggtcctt tttaggttta tgcaagaaaa agatgtattt
     3601 gaacgttatt ataaacaaca cctggcaaga agactgctca caaataaaag tgtttctgat
     3661 gactctgaga aaaatatgat ttctaaatta aagactgagt gtggatgtca attcacatcg
     3721 aaactggaag gaatgtttag ggatatgagc atctcaaaca caactatgga tgagttcagg
     3781 caacatctac aggcaacggg ggtctcttta ggtggtgttg atctcacagt ccgggttctc
     3841 acaacgggat attggcctac tcagtcagcc acaccaaagt gcaacatccc accagcacca
     3901 agacatgctt ttgagatatt tagaaggttc tacttagcca aacacagtgg tcgacagctc
     3961 acactccagc atcacatggg ttctgcagat ctcaatgcca ccttttatgg tccagttaaa
     4021 aaggaagatg gatctgaggt tggtgttggc ggtgcacaag taactggttc taatacacgg
     4081 aagcacatac tgcaagtctc cactttccag atgaccatat taatgctttt taataataga
     4141 gagaagtaca catttgagga aattcaacaa gagacagata tccctgaaag agaacttgtt
     4201 agagccctcc agtccctcgc ctgtggtaaa ccaacacagc gggtcctcac aaaagaaccc
     4261 aagtccaagg agatagaaag tggccacata tttacagtta atgatcaatt cacatctaaa
     4321 ctacacagag tcaagattca aacagttgca gccaaacaag gtgaatccga cccagaaagg
     4381 aaagaaacaa gacagaaagt agatgatgac agaaaacatg agatagaagc tgctatagtg
     4441 cgaataatga agtctaggaa gaagatgcag cacaatgttt tagtagcaga ggtaactcag
     4501 caactgaagg ctcgattctt accaagtcca gttgttatta agaaacgtat tgaaggactt
     4561 attgagagag aatatttggc acgaacacct gaggatcgca aagtatacac atatgtagca
     4621 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     4681 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     4741 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     4801 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     4861 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     4921 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     4981 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     5041 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     5101 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     5161 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     5221 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     5281 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     5341 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     5401 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     5461 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     5521 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     5581 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     5641 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     5701 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     5761 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     5821 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     5881 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     5941 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     6001 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     6061 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6121 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6181 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6241 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6301 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6361 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6421 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6481 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6541 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6601 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6661 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6721 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6781 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6841 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6901 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6961 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7021 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7081 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7141 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7201 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7261 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7321 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
     7381 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7441 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7501 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7561 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7621 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7681 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7741 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7801 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7861 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7921 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
     7981 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8041 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8101 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8161 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8221 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8281 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8341 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8401 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8461 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8521 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8581 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8641 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8701 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8761 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8821 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8881 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8941 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9001 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9061 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9121 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9181 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9241 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9301 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9361 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9421 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9481 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9541 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9601 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9661 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9721 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9781 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9841 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9901 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9961 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10021 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10081 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    10141 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    10201 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    10261 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    10321 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10381 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    10441 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    10501 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    10561 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
