LOCUS       V024739                10577 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024739
VERSION     V024739
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4620
                     /label="Chpf2(NM_133913)"
                     /note="Chpf2(NM_133913)"
                     /gene="Chpf2"
     CDS             4627..4692
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4736..4947
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4960..5228
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5257..5266
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5287..5949
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6019..6072
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6073..6672
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6673..7260
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7334..7567
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7639..7760
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7779..7914
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7952..7968)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7976..7992
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8000..8030)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8045..8066
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8354..8942)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9113..9973)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9974..10078)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10552..10568
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc gactgagctc cctgctggct
     2341 ctgctgcggc cagtgctccc cctcatccta ggactgtctc tgggatgcag cctgagcctc
     2401 ctgcgggttt cctggatcca gggtgaggga gaagatccct gtgtagaggc tgtggggaag
     2461 ccaggagggc cacagaatcc agactcaaaa aatgggctgg accaaggcga tgaagacttc
     2521 agacccagga ttgtgcctta ctacagggat cctaacaagc cctacaagaa ggtgctcagg
     2581 actcggtata tacagacaga gctgggctct cgtgagcgcc tgctggtggc tgtcctgact
     2641 tctcgagcca cactgtccac cctggccgtc gcggttaacc gtacagtagc acatcacttc
     2701 cctcggttac tctacttcac agggcagcga ggggcccggg ctcctgcagg gatgcaggtg
     2761 gtgtctcatg gagatgagcg gcctgcctgg ctcatgtcag agaccctgcg ccaccttcac
     2821 acacactttg gggcagacta tgactggttc ttcatcatgc aggacgacac atatgtgcag
     2881 gcccccagat tggcagctct tgctggtcac cttagcatca accaagacct gtacttaggc
     2941 catgcagagg aattcattgg tacaggagag caggcccggt actgccatgg gggctttggc
     3001 tacctgttgt cacggagtct cctattgcgc ttgcggccac atctggatgg ctgccgaggg
     3061 gacattctca gtgcccggcc tgatgaatgg cttggccgct gcctcatcga cactctgggc
     3121 atcggctgtg tctcacagca ccaggggcaa cagtatcgct ccttcgaact ggccaagaat
     3181 agggaccctg agaaggagga gagccctgct ttcctgagtg ccttcgccgt tcacccggtc
     3241 cccgagggca ccctcatgta ccggctccac aaacgcttca gtgctctgga gttggagcgg
     3301 gcttacagcg aaatagaaca actgcaggcc cagatccgga acctgacggt gctgacccct
     3361 gagggggagg cagggctaag ctggcctgtt gggctcccgg cccctttcac tccacactct
     3421 cgcttcgagg tgctgggctg ggactacttc acggagcaac acaccttcgc ctgtgcagac
     3481 ggggctccca agtgtcccct gcagggggct agcagggcag atgtagggga cgctgtggac
     3541 acagctctgg agcagctcaa tcggcgctat cagccccgcc tgcgcttccg gaagcagcgg
     3601 cttctcaatg gctaccggcg ctttgaccct gcaaggggca tggaatacac cctcgacttg
     3661 ctgctggaag ctgtgactca gcgagggcac agacgctccc tggcccgcag agtgagcctg
     3721 ctgaggccac tgagcagagt ggaaatcctg cctatgccct atgtcaccga ggctacccgg
     3781 gtgcaactgg tgctgccact cctggtggct gaagctccgg ccgccttggc ttttcttgag
     3841 gcctttgctg ccagtgttct ggagcctcga gaacatgcat tgctcaccct gttgttggtc
     3901 tatgggcccc gggaaggccg agggggccct gacccatttc tcggagtgaa ggctgcagca
     3961 gctgagttag aacgacggta cccaggggcg aggctggcct ggctcgctgt gcgagcagag
     4021 gccccttcgc aggtgcgact catggatgtc atctccaaga aacaccctgt ggacacactc
     4081 ttcttcctta ccaccgtgtg gacaagacct gggtcggaag tcctcaaccg ctgccgtatg
     4141 aatgcaatct cgggctggca ggccttcttt ccggtccact ttcaggagtt caaccctgct
     4201 ctgtcaccac agagatctcc cccaggtgtc cctggggctg atcccccctc ccctggtgtg
     4261 gacccttcta ggggggctcc ggtcgggggc agatttgacc ggcaggcttc agcagagggc
     4321 tgcttctaca atgctgacta cctggcagcc cgcgcccggc tggctggcga actggcaggc
     4381 caggaagagg aggaagccct ggaggggctg gaggtgatgg atgttttcct ccggttctca
     4441 gggctccacc tctttcgagc cgtagagcca gggctggtgc agaagttctc tctgcgggac
     4501 tgtagccccc ggctcagcga ggaactgtac caccgttgtc gcctcagcaa tctggagggg
     4561 ctgggggccc gtacccagct agccatggct ctgtttgaac aggaacaggc caacagcact
     4621 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     4681 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     4741 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     4801 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     4861 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     4921 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     4981 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     5041 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     5101 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     5161 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     5221 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     5281 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     5341 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     5401 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     5461 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     5521 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     5581 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     5641 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     5701 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     5761 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     5821 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     5881 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     5941 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     6001 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     6061 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6121 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6181 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6241 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6301 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6361 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6421 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6481 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6541 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6601 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6661 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6721 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6781 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6841 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6901 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6961 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7021 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     7081 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7141 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7201 cttccgcgtc tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc
     7261 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7321 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct
     7381 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7441 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7501 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7561 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7621 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7681 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7741 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7801 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7861 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
     7921 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7981 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8041 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8101 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8161 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8221 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8281 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8341 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8401 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8461 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8521 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8581 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8641 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8701 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8761 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8821 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8881 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8941 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9001 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9061 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9121 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9181 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9241 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9301 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9361 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9421 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9481 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9541 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9601 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9661 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9721 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9781 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9841 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9901 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9961 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10021 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10081 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
    10141 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
    10201 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
    10261 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
    10321 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    10381 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
    10441 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    10501 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    10561 cggccagtgc caagctg
//
