LOCUS       V042280                13756 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042280
VERSION     V042280
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7782
                     /label="Cdk5rap2(NM_145990)"
                     /note="Cdk5rap2(NM_145990)"
                     /gene="Cdk5rap2"
     CDS             7789..7854
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7898..8109
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8122..8390
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      8419..8428
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             8449..9111
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             9181..9234
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             9235..9834
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9835..10423
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10497..10730
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10802..10923
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10963..11098
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11131..11147)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11155..11171
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11179..11209)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11224..11245
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11533..12121)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12292..13152)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13153..13257)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13731..13747
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg actcggggat ggaagaggaa
     2341 ggcgccctgc ctgggaccct cagcggttgc agtggccttc accctgttct gccaagtgac
     2401 ctggatgtca tcagtgacac ttctggcttg ggaaatggag tgctcccaag catgtcagaa
     2461 gaaaaggttt ctcccaccag agcccggaac atgaaggact ttgaaaacca aatcactgag
     2521 ctgaagaagg agaacttcaa tctaaagctc cggatctact tcctggagga gagaatccag
     2581 caggagtttg ctggccccac cgagcacatc tacaagacga acattgaact caaggtggaa
     2641 gtggaaagtc tgaagcgcga gctccaggag aaagatcagt tgctcgtcaa agcctccaaa
     2701 gcggtggaga gcctagcaga acggggtggt tctgaagtcc agcgggtgaa agaagatgca
     2761 aggaagaagg tgcagcaggt ggaagacctc ctaacgaaaa ggatacatct tctagaagaa
     2821 gatgtgaaag ccgcccaggc agaactggag aaggcctttg cggggacaga aacagagaag
     2881 gctcttcggc tcagcttaga aagcaagctc tcggcgatga agaagatgca ggagggggac
     2941 ctggagatga ctctggctct ggaagagaag gacagactga ttgaggagtt gaagctgtct
     3001 ttgaagagca aagaagcttt aattcagtgc cttaaagagg aaaaatctca gatggcatct
     3061 cctgacgaga acgtgtcatc tggagaactc cgaggacttt ctgccactct gagggaagaa
     3121 aaagagagag acgctgagga atggcagaag gagagaaacc actttgaaga gaggatccag
     3181 gcacttcagg aggacctgag agagaaggaa agagaaattg ctacagagaa gaaaaataac
     3241 ttaaagagtt ataaagccat tcagggtcta accatggcat taaaatcaaa ggaaagagag
     3301 gttgaagaac ttgattccaa aatcaaagag gtcactacgg actccaccaa aggcagagag
     3361 gatcccttaa aaacccagat ccccagattc cagctaagag aggggtctga ggactgtgaa
     3421 gctgcgctgg tagagaagga agccctattg gccaagctac actcagaaaa tgtcacaaag
     3481 agcactgaga accataggtt gctcaggaat gttaaaaagg tcacccagga gttgaatgac
     3541 ttgaagaagg agaagttgag actagagcag gccctggagg aagcccacca agaggggaac
     3601 agaggagccc gaaccattca cgaccttaga aatgaagttg agaaactacg caaggaagtg
     3661 agtgagagag agaaggctgt ggaaaaacat tacaagagtc ttccaggtga aagcaagaca
     3721 aagtttcata ctcaagaaca agtggtcaga agtctgacag gcagtggcag tcaggaggac
     3781 ctgctgcttc agaaatccaa tgaaaaagat ttggaagcaa tacagcagaa ctgctattta
     3841 atgactgcag aggagctcaa gtttgggagc gatggcttaa taacagaaaa gtgttctcag
     3901 cagtcaccag acagcaaact catcttctcc aaggaaaagc aacaatcaga gtatgaaggg
     3961 ctgactgggg acttaaaagc agaacagaac atctatgccc atctggcaaa gactcaggac
     4021 acagacagtg tcagcaacct gcaggctgag ctgaaagagg tccttgccct tcggaagcag
     4081 ctggagcagg atgttcttgc ctaccggaac ctgcagaagg ccctgcagga gcagctcagt
     4141 gagattcgga gtcgagaaga agagccgttt tcattttata gcgatcaaac atcgtatctg
     4201 agtatttgcc ttgaagagca caatcagttc caattggagc atttttctca agaagaactc
     4261 aagaaaaagg tcagtgacct catacagctt gtgaaggacc tgcacacaga caaccagcac
     4321 ctgaagaaaa ccatttttga tctctcttct gtgggtttcc agggaagtga cagacttgag
     4381 ttaacaaaac aagaagagct tgtggctagc aaggaggatg aggacacact caaatttgaa
     4441 gcggacgttg agactccttt ccagagtgac cagcatttgg agcaaagtcg tgagatcatg
     4501 gaggactatg ctgagggtgg ctgcaaaagt ggatatggaa ggcacatgga ttccaatatt
     4561 ttaggtcatg atggggccca aactcctggt gcctctgaag agcacaccct agaagatgag
     4621 ctcctgggcc tgcttgccac tctcttcagt aagaaggcca caccgttgct ggaatccagg
     4681 ccagatcttc tgaaagcact cggtgcccta cttttggaac gcatatgctt ggcagagcag
     4741 ggtcgtcctg gagatcacct tgatagtaaa actgagaagg cacttcagca agtagctgtt
     4801 cagttgagag atgaacttgg ccattccttc cctgccaact cgttctccaa gtcatacaat
     4861 gaagtcaagt caatgtgggg aaattggctg gtaaagacag gagacgaaga cacggtggag
     4921 ctgaagagtg tttcagtgca gaccatggcc atagaggaca ccccgcatgg attcaaacct
     4981 caaagcaaga gagatgcttg ggcagagaaa caagaagagg ccattttcag cactgagctt
     5041 gagtcagagg cgttgggtga gatgccaggg caacaggcca ctcacctctc attccccagt
     5101 gccatcaacc cggatgccga gaagacgggc ttgttgatcc aattaaagac acctgagtta
     5161 ttggaaaatt tatataatct tcctgcttcg ccagaggtgg tggtggctca actccaaggc
     5221 caggttctag agctgcagag ggagctgaaa gaattcaaaa cccgcaacaa gcaactccat
     5281 gagaagctaa ttctggcaga ggcaatgatg gaggggctgc cggtgcccaa ctctgcgctg
     5341 gtgaatgtgc ctgcagccca ggctgttgtg agaacagcct tccaggacaa cccaggcgag
     5401 caagaaggac ctgaaaccac acagtctgct gggagagaca aggacatgga cagtgaccaa
     5461 tatacaagct ttgagatcga ttctgaaatc tgtccacctg atgaccttgc cttattacca
     5521 gcatgcaaag agaatcttga agacctccta ggcccatcct ccatagccac ttacctggat
     5581 tccaagagtc agctctctgt taaagtcagc gtgaatggaa ctgaccagtc tgagaatatc
     5641 aatatccctg atgacacaga agacttaaaa cagaaaatcc acgacttgca aaccgagctg
     5701 gaaggctacc ggaacatcat tgtccagcta ctgaagcact ctcagtgcag tgaggctatt
     5761 atcacagttt tgtgtgggac agaaggggcc caagatggct tgaacaagcc taagggccat
     5821 attgatgagg aagagatgac attttcaagt ttgcaccaag tacgatatgt gaagcatatg
     5881 aaaatcctcc gtccactaac cccggagatg attgatggca agatgctaga gagcctcaag
     5941 cagcagctgg tggaccagga gcaggagcta cagaaggagc aggacttgaa cttggaactt
     6001 ttcggtgaaa tccatgacct gcagaacaag ttccaagacc tctcgccatc aagatacgat
     6061 tcattggttc agtcccaagc gagggagctc tcccttcagc gacagcaaat taaggatagc
     6121 catggcatct gtgtcattta ccgtcaacac atgagcacca tgatcaaggc gtttgaggag
     6181 cttctgcagg ccagtgatgt ggacagttgc gtggccgagg gcttccggga gcagctgaca
     6241 cagtgtgctg gactgctaga gcaactggag aggctgtttc tccatggaaa atcagccaga
     6301 gtggaaccac atccccagaa tgaactgctc aaggggttaa ggactgtgga aggcaactta
     6361 ccctaccacc atctcctgcc tgaatctcct gagccttctg cctcccatgc tctgtcagat
     6421 gatgaaatgt ctgagaagtc cttcctgtct agagacccga agccagacag cgatactgag
     6481 aagtacccag caatggcgag ccattttcct caagacttac taatggaaca catacaggaa
     6541 attcgaactt tgagaaagca tttggaagaa tctattaaaa caaatgagaa gctccggaaa
     6601 cagctggaac ggcaggggtc tgagactgac caaggctcca gaaatgtttc tgcttgtggc
     6661 ttagcactgc acagctctct gacgtcagag atacatttcc tgaggaagca gaacgaggcc
     6721 ctcagtatga tgctcgagaa ggggtccaaa gataaacaga aagagagtga aaaattacgt
     6781 gagtccctgg ctaggaaagc tgagagtctt gagcaacttc agctagagta caccagtgta
     6841 agggaagaaa atgaaaggtt acagagagac atcattgaga aggagagaca caaccaggag
     6901 ctgactgagg aggtctgcag cagccgccag gaactgagca gggtgcagga agaggcgaag
     6961 tcaagacagc agctgctctc acagaaggac aagctgcttc agtccctcca aatggagctc
     7021 aaggtgtatg agaaactggc cgaggaacat ccgaggctac agcaagatgg cagcaaatgt
     7081 cctgaggcca gcgacaattc atttgatcta tttgaatcca cccaggcaat ggcacctaaa
     7141 tcagcttctg agactcctct gctctcagga actgatgttg actcgctctc ctgtgacagt
     7201 accagttctg ccaccagccc tactagcatg ccctgcctgg ttgctggcca ccacatgtgg
     7261 gccagcaaga gtggccacca tatgctgggc ctgattgagg actatgatgc cctctataag
     7321 cagatcagct ggggccagac actgcttgcc aagatggatg ttcaaaccca agaggctctg
     7381 agccccacaa gtcacaagct gggaccaaag ggttcttcat ccgtgcctct gagcaagttt
     7441 ctttccagta tgaacacagc caagctggtc ctggaaaaag catccaggtt gctgaagctc
     7501 ttctggaggg tctcagtccc caccaacggc cagtgttccc ttcactgtga gcagattgga
     7561 gaaatgaagg cagagaacac gaaactacac aaaaaattgt ttgaacaaga aaagaagctg
     7621 cagaacacag caaaactatt gcagcagagc aagcaccagg agaaagtcat ctttgatcag
     7681 ttggtcatca cccaccaagt ccttcggaag gccaggggaa acctggagct caggcctggg
     7741 gccacccgtc caggagcatc cagtcccagc agaccgggct caggatccga ctacaaagac
     7801 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     7861 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     7921 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     7981 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     8041 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     8101 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     8161 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     8221 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     8281 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     8341 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     8401 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     8461 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     8521 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     8581 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     8641 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     8701 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     8761 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     8821 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     8881 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     8941 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     9001 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     9061 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     9121 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     9181 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     9241 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     9301 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     9361 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     9421 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     9481 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     9541 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     9601 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     9661 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     9721 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     9781 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     9841 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     9901 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     9961 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
    10021 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
    10081 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
    10141 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
    10201 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
    10261 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
    10321 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
    10381 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
    10441 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
    10501 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
    10561 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    10621 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    10681 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
    10741 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
    10801 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    10861 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10921 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
    10981 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    11041 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    11101 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
    11161 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    11221 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    11281 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    11341 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    11401 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    11461 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    11521 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    11581 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    11641 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    11701 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    11761 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    11821 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    11881 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    11941 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    12001 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    12061 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    12121 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    12181 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    12241 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    12301 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    12361 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    12421 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    12481 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    12541 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    12601 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    12661 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    12721 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    12781 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    12841 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    12901 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    12961 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    13021 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    13081 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    13141 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    13201 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    13261 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    13321 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    13381 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    13441 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    13501 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    13561 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    13621 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    13681 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    13741 ggccagtgcc aagctg
//
