LOCUS V024284 10625 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024284 VERSION V024284 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..4668 /label="Cdk11b(NM_007661)" /note="Cdk11b(NM_007661)" /gene="Cdk11b" CDS 4675..4740 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4784..4995 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 5008..5276 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5305..5314 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5335..5997 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 6067..6120 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 6121..6720 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6721..7308 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7382..7615 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7687..7808 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7827..7962 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(8000..8016) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8024..8040 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8048..8078) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8093..8114 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8402..8990) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9161..10021) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10022..10126) /gene="bla" /label="AmpR promoter" primer_bind 10600..10616 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gtgatgaaaa ggactcttgg 2341 aaagtgaaaa cgttagatga aattctccag gaaaagaaac gaaggaaaga acaagaggag 2401 aaagcagaga taaaacgctt aaaaaattct gatgaccgcg attccaaaag ggattccctt 2461 gaggaggggg agctgagaga tcaccgaatg gagatcacaa tcaggaactc accatataga 2521 agagaggatt ctatggaaga cagaggagag gaggatgatt ctctggccat caaaccaccc 2581 cagcaaatgt ctcggaaaga aaaggctcat cacagaaaag acgagaaaag aaaagagaaa 2641 cgtcgacatc gtagccattc agcagaggga gggaaacatg ccagagtgaa agagaaagaa 2701 agggagcacg aacgccggaa acgccaccga gaagaacaag ataaagctcg aagggagtgg 2761 gaaagacaga agaggaggga aatggcgaga gaacattcca gaagagagag ggaccgcctg 2821 gagcagttag aaaggaagag ggagcgggag cgcaagctga gggagcagca gaaggagcag 2881 cgggagcaga aggagcggga acggagggca gaggagcgcc gcaaagagag agaagcgcgt 2941 agggaagtct ctgcacatca ccgtaccatg agggaggagt acagtgataa ggggaaggtt 3001 ggccactgga gccgcagccc tctgaggcca ccaagagagc gctttgagat gggagacaac 3061 cggaagccag taaaagaaga gaaggtggaa gagagagact tgttgtcaga cctccaagac 3121 atcagtgaca gcgagaggaa aaccagctca gctgagtctt catcagcaga atcaggctca 3181 ggttctgaag aggaggagga ggaagaagaa gaggaagaag aagaagaagg gagcaccagt 3241 gaagaatcag aggaagaaga ggaagaagaa gaggaggagg aagaagagga gactgggagc 3301 aactctgagg aggcctctga acagtccgca gaagaagtca gtgatgagga aatgagtgaa 3361 gatgaagaca gagaaaacga gaaccacatc ttggttgttc cagagtcacg atttgaccga 3421 gattctgggg acagtgaaga aggggaggaa gaagttggtg aagggactcc acagagcagt 3481 gcccccaccg aaggagacta tgttcctgac tctccagcac tgtcacctat tgagctaaaa 3541 caagaactgc ccaaatacct gccagccctg cagggatgca ggagtgtaga ggagtttcag 3601 tgtctgaaca ggattgaaga aggcacctat ggggtggtct acagagcaaa ggacaagaaa 3661 acagatgaaa ttgtggctct gaagcggtta aagatggaga aggagaagga aggcttccca 3721 atcacgtcgc tgagggaaat caacaccatc ctcaaggccc agcaccccaa catcgtcacc 3781 gtcagggaaa ttgttgtggg aagtaacatg gacaagatct acattgtgat gaactacgtg 3841 gaacatgacc tcaagagcct aatggagacc atgaagcagc ccttcctgcc aggggaggtg 3901 aagaccctga tgattcagct gctgagtggg gtaaagcacc tccatgacaa ttggatccta 3961 caccgtgacc tgaagacctc taacctcctg ctgagccatg ctggcattct caaggtgggc 4021 gactttgggc tggctcggga gtatggttca cccctaaagg cctacactcc agttgttgta 4081 accctgtggt atcgtgcccc agaactactg cttggtgcta aggaatactc cacagctgtg 4141 gacatgtggt cggtaggctg catatttgga gaactgctga cacagaaacc tctgttccct 4201 gggaagtcag atattgatca gattaacaag attttcaagg acctgggtac tcctagtgag 4261 aaaatctggc ctggctataa tgacctccca gccgtcaaga agatgacctt cagcgagtat 4321 ccctataaca acctccgcaa gagatttggg gctttgttat cagatcaagg ctttgatctc 4381 atgaacaagt tcctgacata ctaccctggc aggaggatca acgcagaaga tggcctcaag 4441 cacgaatatt tccgagagac tcccctcccc atcgacccat ccatgttccc cacgtggcct 4501 gccaagagtg agcagcagag agtgaagcga ggcacgagtc cacggcctcc tgagggcggc 4561 ctgggctaca gccagctggg tgatgatgac ctgaaggaga cgggcttcca cctcaccacc 4621 accaaccagg gagcctcagc tgcaggccct ggcttcagcc tcaagttcgg atccgactac 4681 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag 4741 tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 4801 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 4861 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 4921 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 4981 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 5041 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 5101 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 5161 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 5221 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 5281 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 5341 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 5401 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 5461 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 5521 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 5581 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 5641 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 5701 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 5761 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 5821 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 5881 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 5941 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 6001 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 6061 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 6121 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 6181 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 6241 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 6301 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 6361 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 6421 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 6481 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 6541 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 6601 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 6661 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 6721 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 6781 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 6841 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 6901 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 6961 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 7021 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 7081 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 7141 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 7201 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc 7261 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta 7321 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 7381 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 7441 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 7501 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 7561 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 7621 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 7681 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 7741 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 7801 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 7861 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 7921 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 7981 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 8041 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 8101 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 8161 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 8221 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 8281 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 8341 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 8401 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 8461 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 8521 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 8581 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 8641 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 8701 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 8761 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 8821 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 8881 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 8941 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 9001 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 9061 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 9121 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 9181 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 9241 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 9301 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 9361 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 9421 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 9481 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 9541 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 9601 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 9661 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 9721 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 9781 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 9841 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 9901 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 9961 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 10021 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 10081 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 10141 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 10201 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 10261 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 10321 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 10381 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 10441 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 10501 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 10561 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 10621 agctg //