LOCUS       V030421                12619 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030421
VERSION     V030421
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6645
                     /label="CPSF1(NM_013291)"
                     /note="CPSF1(NM_013291)"
                     /gene="CPSF1"
     CDS             6652..6717
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6761..6972
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6985..7253
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7282..7291
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7312..7974
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8044..8097
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8098..8697
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8698..9286
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9360..9593
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9665..9786
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9826..9961
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9994..10010)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10018..10034
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10042..10072)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10087..10108
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10396..10984)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11155..12015)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12016..12120)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12594..12610
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt acgccgtgta caaacaggcg
     2341 catccgccca ccggtctgga gttctccatg tactgcaact tcttcaacaa cagcgagcgc
     2401 aacctggtag tggccgggac ctcgcagctc tacgtgtacc gcctcaaccg cgacgccgag
     2461 gctctgacca agaatgacag gagcacagag gggaaggccc accgggagaa gctcgagctt
     2521 gctgcctcct tctccttctt tggcaacgtc atgtccatgg ccagcgtgca gctggcagga
     2581 gccaagcggg atgccctgct cctaagcttc aaggatgcca agctgtctgt ggtggagtac
     2641 gacccgggca cccatgacct gaagaccctg tcactgcact actttgagga gcctgagctt
     2701 cgggacgggt ttgtgcagaa tgtacacacg ccgcgagtgc gggtggaccc cgacgggcgc
     2761 tgtgcagcca tgcttgtcta cggcacgcgg ctggtggtcc tgcccttccg cagggagagc
     2821 ctggctgagg agcacgaggg gctcgtgggt gaggggcaga ggtccagctt cctgcccagc
     2881 tacatcatcg acgtgcgggc cctagacgag aagctgctca acatcatcga cctgcagttc
     2941 ctgcatggct actacgagcc taccctcctc atcctgtttg agcccaacca gacctggcct
     3001 gggcgcgtgg ccgtgcggca ggacacgtgc tccattgtgg ccatctcact gaacatcacg
     3061 cagaaggtgc accccgtcat ctggtccctc accagcctgc cctttgactg cacccaggct
     3121 ctggctgtgc ccaagcccat aggtggggtg gtggtgtttg ccgtcaactc gctgttgtac
     3181 ctgaaccaga gcgtcccccc gtatggcgtg gctctcaaca gcctcaccac aggaaccacg
     3241 gctttcccgc ttcgcaccca ggagggtgtg cggatcaccc tggactgcgc ccaggccacc
     3301 ttcatctcct acgacaagat ggtcatctcc ctcaagggcg gcgagatcta cgtgctgacc
     3361 ctcatcaccg acggcatgcg cagtgtccga gcgttccact ttgacaaggc ggccgccagc
     3421 gtcctcacca ccagcatggt caccatggag cccgggtacc tgttcctggg ttctcgcctg
     3481 ggcaattccc tcctcctcaa gtacacggag aagctgcagg agcccccggc cagtgctgtc
     3541 cgtgaggctg ccgacaagga agagcctccc tcaaagaaga agcgagtgga tgcgacggcc
     3601 ggctggtcag ctgcgggtaa gtcggtgccg caggatgagg tggacgagat tgaagtgtac
     3661 ggcagcgagg cccagtcggg aacacagctg gccacctact cctttgaggt gtgtgacagc
     3721 atcctgaaca ttggaccctg tgccaatgcc gccgtgggcg agcctgcctt cctctctgaa
     3781 gagtttcaga acagccccga gccggacctg gagattgtgg tttgctccgg ccacgggaag
     3841 aacggggctt tgtcggtgct gcagaagagc atccggcccc aggtggtgac aacctttgag
     3901 cttcccggct gctatgacat gtggacagtc atcgccccgg tgcgtaagga ggaggaggac
     3961 aatcccaagg gggagggcac agagcaggaa cccagcacca cccctgaagc agacgacgac
     4021 ggccgcagac acggattcct gattctgagc cgggaagact ccaccatgat cctgcagacg
     4081 gggcaggaga tcatggagct ggacaccagt ggcttcgcca ctcagggccc cacggtcttt
     4141 gctgggaaca tcggggacaa ccgctacatt gtccaagtgt caccactggg catccgcctg
     4201 ctggaaggag tgaatcagct gcacttcatc cccgtggacc tgggcgcccc catcgtgcag
     4261 tgcgccgtgg ccgaccccta tgtggtcatc atgagtgccg agggccacgt caccatgttc
     4321 ctgctgaaga gtgactccta cggtggccgc caccaccgcc tggcgctgca caagcccccg
     4381 ctgcaccatc agtccaaggt gattacgctg tgcctgtacc gagacctcag cggcatgttc
     4441 accactgaga gccgcctggg tggggcccgt gacgagctcg ggggccgcag tggcccggag
     4501 gccgagggcc tgggctcaga gactagcccc acagtggatg acgaggagga gatgctgtat
     4561 ggggattcgg gctccctctt cagccccagc aaggaggagg cccgaagaag cagccagccc
     4621 cctgctgacc gggaccctgc acccttccgg gcagagccta cccactggtg cctgctggtg
     4681 cgggagaatg gcaccatgga gatctaccag cttcccgact ggcggctggt gttcctggtg
     4741 aagaacttcc ctgtggggca gcgggtcctt gtggacagct cctttggaca gcccactaca
     4801 cagggcgagg cccgcaggga ggaggccacg cgccaggggg agctgcccct cgtcaaggag
     4861 gtgctgctgg tggcgctggg cagccgccag agcaggccct acctgctggt gcatgtggac
     4921 caagagctgc ttatctacga ggccttcccc cacgactctc agctcggcca gggcaatctc
     4981 aaagtccgct ttaagaaggt ccctcacaac atcaacttcc gtgagaagaa gccaaagcca
     5041 tccaagaaga aagcagaagg tggcggcgca gaggaggggg ctggggcccg gggccgcgtg
     5101 gcgcgtttcc gctacttcga ggatatttat ggctactcag gggtcttcat ctgcggcccc
     5161 tcccctcact ggctcttggt gaccggccga ggggctctgc ggctacaccc catggccatc
     5221 gacggcccgg tcgactcttt cgctccattc cacaatgtca actgtccccg cggcttcctg
     5281 tacttcaaca gacagggcga gctgaggatc agtgtcctgc ctgcctacct gtcctatgat
     5341 gccccatggc ctgtcaggaa gatcccgctg cgctgcacgg cccactatgt ggcttaccac
     5401 gtggagtcta aggtgtatgc tgtggccacc agcaccaaca cgccgtgtgc ccgcatccca
     5461 cgcatgactg gcgaggagaa ggagtttgag accatcgaga gagatgagcg gtacatccac
     5521 ccccagcagg aggccttctc catccagctc atctccccgg tcagctggga ggctattccc
     5581 aatgccagga tcgagctgca ggagtgggag catgtgacct gcatgaagac agtgtctctg
     5641 cgcagtgagg agaccgtgtc gggcctcaaa ggctacgtgg ccgccgggac ctgcctcatg
     5701 cagggggagg aggtcacgtg ccgagggcgg atcttgatca tggatgtgat tgaggtggtg
     5761 cccgagcctg gccagccctt gaccaagaac aagttcaaag tcctttacga gaaggagcag
     5821 aaggggcccg tgaccgccct gtgccactgc aatggccacc tggtgtcggc catcggccag
     5881 aagattttcc tgtggagcct gcgggccagc gagctgacgg gcatggcctt catcgacacg
     5941 cagctctaca tacaccagat gatcagcgtc aagaacttca tcctggcagc cgacgtcatg
     6001 aagagcattt cgctgctgcg ctaccaggag gaaagcaaga cgctgagcct ggtgtcgcgg
     6061 gatgccaagc ccctggaggt gtacagcgtg gacttcatgg tggacaatgc ccagctgggt
     6121 tttctggtgt ctgaccgcga ccgcaacctc atggtgtaca tgtacctgcc cgaagccaag
     6181 gagagtttcg ggggcatgcg cctgctgcgt cgggcagact tccacgtggg tgcccacgtg
     6241 aacacgttct ggaggacccc gtgccggggg gccactgaag ggctcagcaa aaagtcggtc
     6301 gtgtgggaga ataagcacat cacgtggttt gccaccctgg acggcggcat cgggctgctg
     6361 ctgcccatgc aggagaagac ctaccggcgg ctgctgatgc tgcagaacgc gctgaccacc
     6421 atgctgccac accacgccgg cctcaacccc cgcgccttcc ggatgctgca cgtggaccgc
     6481 cgcaccctcc agaatgccgt gcgcaacgtg ctggatgggg agctgctcaa ccgctacctg
     6541 tacctgagca ccatggagcg cagcgagcta gccaagaaga tcggcaccac accagacata
     6601 atcctggacg acttgctgga gacggaccgc gtcaccgccc acttcggatc cgactacaaa
     6661 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag
     6721 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     6781 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     6841 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     6901 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     6961 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     7021 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     7081 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     7141 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     7201 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     7261 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg
     7321 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc
     7381 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc
     7441 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc
     7501 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc
     7561 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc
     7621 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac
     7681 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc
     7741 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc
     7801 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc
     7861 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc
     7921 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc
     7981 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct
     8041 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     8101 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     8161 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     8221 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     8281 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     8341 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     8401 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     8461 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     8521 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     8581 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     8641 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     8701 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8761 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8821 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8881 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     8941 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     9001 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     9061 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     9121 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     9181 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     9241 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     9301 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     9361 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct
     9421 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9481 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9541 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     9601 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     9661 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9721 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9781 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     9841 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9901 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9961 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
    10021 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    10081 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    10141 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    10201 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    10261 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    10321 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    10381 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    10441 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    10501 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    10561 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    10621 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    10681 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    10741 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    10801 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    10861 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    10921 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    10981 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    11041 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    11101 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    11161 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    11221 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    11281 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    11341 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    11401 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    11461 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    11521 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    11581 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    11641 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    11701 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    11761 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    11821 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    11881 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    11941 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    12001 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    12061 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    12121 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    12181 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    12241 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    12301 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    12361 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    12421 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    12481 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    12541 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    12601 gacggccagt gccaagctg
//
