LOCUS       V033104                10535 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033104
VERSION     V033104
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4716
                     /label="CLCN7(NM_001287)"
                     /note="CLCN7(NM_001287)"
                     /gene="CLCN7"
     CDS             4753..5463
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     promoter        5505..5716
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5729..5997
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6024..6623
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6630..7218
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7292..7525
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7597..7718
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7737..7872
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7910..7926)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7934..7950
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7958..7988)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8003..8024
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8312..8900)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9071..9931)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9932..10036)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10510..10526
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggccaac gtctctaaga aggtgtcctg gtccggccgg
     2341 gaccgggacg acgaggaggc ggcgccgctg ctgcggagga cggcgcggcc cggcgggggg
     2401 acgccgctgc tgaacggggc tgggcctggg gctgcgcgcc agtcaccacg ttctgcgctt
     2461 ttccgagtcg gacatatgag cagcgtggag ctggatgatg aacttttgga cccggatatg
     2521 gaccctccac atcccttccc caaggagatc ccacacaacg agaagctcct gtccctcaag
     2581 tatgagagct tggactatga caacagtgag aaccagctgt tcctggagga ggagcggcgg
     2641 atcaatcaca cggccttccg gacggtggag atcaagcgct gggtcatctg cgccctcatt
     2701 gggatcctca cgggcctcgt ggcctgcttc attgacatcg tggtggaaaa cctggctggc
     2761 ctcaagtaca gggtcatcaa gggcaatatc gacaagttca cagagaaggg cggactgtcc
     2821 ttctccctgt tgctgtgggc cacgctgaac gccgccttcg tgctcgtggg ctctgtgatt
     2881 gtggctttca tagagccggt ggctgctggc agcggaatcc cccagatcaa gtgcttcctc
     2941 aacggggtga agatccccca cgtggtgcgg ctcaagacgt tggtgatcaa agtgtccggt
     3001 gtgatcctgt ccgtggtcgg gggcctggcc gtgggaaagg aagggccgat gatccactca
     3061 ggttcagtga ttgccgccgg gatctctcag ggaaggtcaa cgtcactgaa acgagatttc
     3121 aagatcttcg agtacttccg cagagacaca gagaagcggg acttcgtctc cgcaggggct
     3181 gcggccggag tgtcagcggc gtttggagcc cccgtgggtg gggtcctgtt cagcttggag
     3241 gagggtgcgt ccttctggaa ccagttcctg acctggagga tcttctttgc ttccatgatc
     3301 tccacgttca ccctgaattt tgttctgagc atttaccacg ggaacatgtg ggacctgtcc
     3361 agcccaggcc tcatcaactt cggaaggttt gactcggaga aaatggccta cacgatccac
     3421 gagatcccgg tcttcatcgc catgggcgtg gtgggcggtg tgcttggagc agtgttcaat
     3481 gccttgaact actggctgac catgtttcga atcaggtaca tccaccggcc ctgcctgcag
     3541 gtgattgagg ccgtgctggt ggccgccgtc acggccacag ttgccttcgt gctgatctac
     3601 tcgtcgcggg attgccagcc cctgcagggg ggctccatgt cctacccgct gcagctcttt
     3661 tgtgcagatg gcgagtacaa ctccatggct gcggccttct tcaacacccc ggagaagagc
     3721 gtggtgagcc tcttccacga cccgccaggc tcctacaacc ccctgaccct cggcctgttc
     3781 acgctggtct acttcttcct ggcctgctgg acctacgggc tcacggtgtc tgccggggtc
     3841 ttcatcccgt ccctgctcat cggggctgcc tggggccggc tctttgggat ctccctgtcc
     3901 tacctcacgg gggcggcgat ctgggcggac cccggcaaat acgccctgat gggagctgct
     3961 gcccagctgg gcgggattgt gcggatgaca ctgagcctga ccgtcatcat gatggaggcc
     4021 accagcaacg tgacctacgg cttccccatc atgctggtgc tcatgaccgc caagatcgtg
     4081 ggcgacgtct tcattgaggg cctgtacgac atgcacattc agctgcagag tgtgcccttc
     4141 ctgcactggg aggccccggt cacctcacac tcactcactg ccagggaggt gatgagcaca
     4201 ccagtgacct gcctgaggcg gcgtgagaag gtcggcgtca ttgtggacgt gctgagcgac
     4261 acggcgtcca atcacaacgg cttccccgtg gtggagcatg ccgatgacac ccagcctgcc
     4321 cggctccagg gcctgatcct gcgctcccag ctcatcgttc tcctaaagca caaggtgttt
     4381 gtggagcggt ccaacctggg cctggtacag cggcgcctga ggctgaagga cttccgagac
     4441 gcctacccgc gcttcccacc catccagtcc atccacgtgt cccaggacga gcgggagtgc
     4501 accatggacc tctccgagtt catgaacccc tccccctaca cggtgcccca ggaggcgtcg
     4561 ctcccacggg tgttcaagct gttccgggcc ctgggcctgc ggcacctggt ggtggtggac
     4621 aaccgcaatc aggttgtcgg gttggtgacc aggaaggacc tcgccaggta ccgcctggga
     4681 aagagaggct tggaggagct ctcgctggcc cagacgggat ccggtggagg cggatcagga
     4741 ggcggtggat ctatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     4801 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     4861 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     4921 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     4981 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     5041 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     5101 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     5161 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     5221 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     5281 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     5341 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     5401 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     5461 taggcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc
     5521 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag
     5581 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     5641 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     5701 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg
     5761 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg
     5821 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct
     5881 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac
     5941 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa
     6001 gctgtgaccg gcgcctacgc tagatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     6061 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     6121 cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     6181 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     6241 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6301 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6361 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6421 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     6481 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     6541 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     6601 tgacccgcaa gcccggtgcc tgagtcgaca atcaacctct ggattacaaa atttgtgaaa
     6661 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6721 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6781 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6841 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6901 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6961 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7021 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7081 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7141 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7201 tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat
     7261 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa
     7321 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7381 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7441 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7501 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7561 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     7621 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7681 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     7741 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7801 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7861 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt
     7921 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7981 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8041 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8101 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8161 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8221 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8281 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8341 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8401 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8461 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8521 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8581 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8641 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8701 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     8761 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8821 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8881 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8941 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9001 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9061 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9121 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9181 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9241 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9301 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9361 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9421 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9481 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9541 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9601 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9661 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9721 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9781 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9841 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9901 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9961 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10021 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    10081 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
    10141 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    10201 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    10261 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    10321 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
    10381 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    10441 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    10501 cacgacgttg taaaacgacg gccagtgcca agctg
//
