LOCUS V039194 9706 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039194 VERSION V039194 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4536 /label="CHUK(NM_001278)" /note="CHUK(NM_001278)" /gene="CHUK" CDS 4543..4632 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" promoter 4676..4887 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4900..5168 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 5195..5794 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5801..6389 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6463..6696 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6768..6889 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6908..7043 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7081..7097) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7105..7121 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7129..7159) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7174..7195 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7483..8071) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8242..9102) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9103..9207) /gene="bla" /label="AmpR promoter" primer_bind 9681..9697 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggagcgg cccccggggc tgcggccggg cgcgggcggg 2341 ccctgggaga tgcgggagcg gctgggcacc ggcggcttcg ggaacgtctg tctgtaccag 2401 catcgggaac ttgatctcaa aatagcaatt aagtcttgtc gcctagagct aagtaccaaa 2461 aacagagaac gatggtgcca tgaaatccag attatgaaga agttgaacca tgccaatgtt 2521 gtaaaggcct gtgatgttcc tgaagaattg aatattttga ttcatgatgt gcctcttcta 2581 gcaatggaat actgttctgg aggagatctc cgaaagctgc tcaacaaacc agaaaattgt 2641 tgtggactta aagaaagcca gatactttct ttactaagtg atatagggtc tgggattcga 2701 tatttgcatg aaaacaaaat tatacatcga gatctaaaac ctgaaaacat agttcttcag 2761 gatgttggtg gaaagataat acataaaata attgatctgg gatatgccaa agatgttgat 2821 caaggaagtc tgtgtacatc ttttgtggga acactgcagt atctggcccc agagctcttt 2881 gagaataagc cttacacagc cactgttgat tattggagct ttgggaccat ggtatttgaa 2941 tgtattgctg gatataggcc ttttttgcat catctgcagc catttacctg gcatgagaag 3001 attaagaaga aggatccaaa gtgtatattt gcatgtgaag agatgtcagg agaagttcgg 3061 tttagtagcc atttacctca accaaatagc ctttgtagtt tagtagtaga acccatggaa 3121 aactggctac agttgatgtt gaattgggac cctcagcaga gaggaggacc tgttgacctt 3181 actttgaagc agccaagatg ttttgtatta atggatcaca ttttgaattt gaagatagta 3241 cacatcctaa atatgacttc tgcaaagata atttcttttc tgttaccacc tgatgaaagt 3301 cttcattcac tacagtctcg tattgagcgt gaaactggaa taaatactgg ttctcaagaa 3361 cttctttcag agacaggaat ttctctggat cctcggaaac cagcctctca atgtgttcta 3421 gatggagtta gaggctgtga tagctatatg gtttatttgt ttgataaaag taaaactgta 3481 tatgaagggc catttgcttc cagaagttta tctgattgtg taaattatat tgtacaggac 3541 agcaaaatac agcttccaat tatacagctg cgtaaagtgt gggctgaagc agtgcactat 3601 gtgtctggac taaaagaaga ctatagcagg ctctttcagg gacaaagggc agcaatgtta 3661 agtcttctta gatataatgc taacttaaca aaaatgaaga acactttgat ctcagcatca 3721 caacaactga aagctaaatt ggagtttttt cacaaaagca ttcagcttga cttggagaga 3781 tacagcgagc agatgacgta tgggatatct tcagaaaaaa tgctaaaagc atggaaagaa 3841 atggaagaaa aggccatcca ctatgctgag gttggtgtca ttggatacct ggaggatcag 3901 attatgtctt tgcatgctga aatcatggag ctacagaaga gcccctatgg aagacgtcag 3961 ggagacttga tggaatctct ggaacagcgt gccattgatc tatataagca gttaaaacac 4021 agaccttcag atcactccta cagtgacagc acagagatgg tgaaaatcat tgtgcacact 4081 gtgcagagtc aggaccgtgt gctcaaggag ctgtttggtc atttgagcaa gttgttgggc 4141 tgtaagcaga agattattga tctactccct aaggtggaag tggccctcag taatatcaaa 4201 gaagctgaca atactgtcat gttcatgcag ggaaaaaggc agaaagaaat atggcatctc 4261 cttaaaattg cctgtacaca gagttctgcc cggtcccttg taggatccag tctagaaggt 4321 gcagtaaccc ctcagacatc agcatggctg cccccgactt cagcagaaca tgatcattct 4381 ctgtcatgtg tggtaactcc tcaagatggg gagacttcag cacaaatgat agaagaaaat 4441 ttgaactgcc ttggccattt aagcactatt attcatgagg caaatgagga acagggcaat 4501 agtatgatga atcttgattg gagttggtta acagaaggat ccgaacaaaa actaatatca 4561 gaggaagatt tggaacagaa actaatcagt gaagaagatt tagagcaaaa gttaatttcc 4621 gaggaggact tatagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca 4681 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 4741 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 4801 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 4861 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 4921 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 4981 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 5041 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 5101 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 5161 tacagatcca agctgtgacc ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg 5221 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga 5281 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct 5341 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 5401 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 5461 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 5521 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 5581 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 5641 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 5701 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 5761 cacctggtgc atgacccgca agcccggtgc ctgagtcgac aatcaacctc tggattacaa 5821 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 5881 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 5941 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 6001 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 6061 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 6121 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 6181 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat 6241 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 6301 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 6361 tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg 6421 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 6481 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 6541 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 6601 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 6661 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 6721 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 6781 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 6841 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 6901 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 6961 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 7021 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg 7081 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 7141 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 7201 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 7261 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 7321 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 7381 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 7441 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 7501 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 7561 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 7621 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 7681 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 7741 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 7801 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 7861 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 7921 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 7981 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 8041 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 8101 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 8161 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 8221 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 8281 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 8341 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 8401 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 8461 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 8521 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 8581 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 8641 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 8701 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 8761 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 8821 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 8881 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 8941 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 9001 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 9061 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 9121 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 9181 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 9241 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 9301 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 9361 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 9421 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 9481 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 9541 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 9601 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 9661 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg //