LOCUS       V032994                 9927 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032994
VERSION     V032994
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2808..3953
                     /label="CHEK1(NM_001330428)"
                     /note="CHEK1(NM_001330428)"
                     /gene="CHEK1"
     CDS             3960..4025
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4069..4280
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4293..4561
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4590..4599
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4620..5282
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5352..5405
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5406..6005
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6006..6594
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6668..6901
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6973..7094
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7134..7269
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7302..7318)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7326..7342
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7350..7380)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7395..7416
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7704..8292)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8463..9323)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9324..9428)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9902..9918
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc tagctaagaa ccccaaggaa gagtcccagc
     2341 ccttcctttc gcctctgggg gcagtttggc cccgccccgg cctttgggac gggtggggct
     2401 gtggatgggg atgggaattg gaattgagca attgggagga ggcgctgccg caggcgtttt
     2461 ctgccccata ccgctcccta tatcctcttc ctctcttccc cagaccccca cctctccctc
     2521 ctccttcccc agtcgttcgc cggaaagcat ttgtctccca cctcttcata acaacaatta
     2581 atttcctctg gggcctgagg agggcagaat ttcaaccttc ggtgtgcttg ggagtggcga
     2641 ttgtgattta cacgacaaaa tggtcaggtt tagaatagtt gttcgtggtt gaaagacttg
     2701 gataaatgtg gatgagatag aaggggaagc cgaggtgctc ggtggagtca tggcagtgcc
     2761 ctttgtggaa gactgggact tggtgcaaac cctgggagaa ggtgcctatg gagaagccag
     2821 acataggcat gcctgaacca gatgctcaga gattcttcca tcaactcatg gcaggggtgg
     2881 tttatctgca tggtattgga ataactcaca gggatattaa accagaaaat cttctgttgg
     2941 atgaaaggga taacctcaaa atctcagact ttggcttggc aacagtattt cggtataata
     3001 atcgtgagcg tttgttgaac aagatgtgtg gtactttacc atatgttgct ccagaacttc
     3061 tgaagagaag agaatttcat gcagaaccag ttgatgtttg gtcctgtgga atagtactta
     3121 ctgcaatgct cgctggagaa ttgccatggg accaacccag tgacagctgt caggagtatt
     3181 ctgactggaa agaaaaaaaa acatacctca acccttggaa aaaaatcgat tctgctcctc
     3241 tagctctgct gcataaaatc ttagttgaga atccatcagc aagaattacc attccagaca
     3301 tcaaaaaaga tagatggtac aacaaacccc tcaagaaagg ggcaaaaagg ccccgagtca
     3361 cttcaggtgg tgtgtcagag tctcccagtg gattttctaa gcacattcaa tccaatttgg
     3421 acttctctcc agtaaacagt gcttctagtg aagaaaatgt gaagtactcc agttctcagc
     3481 cagaaccccg cacaggtctt tccttatggg ataccagccc ctcatacatt gataaattgg
     3541 tacaagggat cagcttttcc cagcccacat gtcctgatca tatgcttttg aatagtcagt
     3601 tacttggcac cccaggatcc tcacagaacc cctggcagcg gttggtcaaa agaatgacac
     3661 gattctttac caaattggat gcagacaaat cttatcaatg cctgaaagag acttgtgaga
     3721 agttgggcta tcaatggaag aaaagttgta tgaatcaggt tactatatca acaactgata
     3781 ggagaaacaa taaactcatt ttcaaagtga atttgttaga aatggatgat aaaatattgg
     3841 ttgacttccg gctttctaag ggtgatggat tggagttcaa gagacacttc ctgaagatta
     3901 aagggaagct gattgatatt gtgagcagcc agaagatttg gcttcctgcc acaggatccg
     3961 actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg
     4021 acaagtagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
     4081 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
     4141 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     4201 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     4261 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
     4321 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
     4381 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
     4441 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
     4501 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
     4561 ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag agcggcctgc
     4621 ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg
     4681 tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca
     4741 aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc
     4801 acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg
     4861 gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca
     4921 gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc
     4981 ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc
     5041 tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg
     5101 acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc
     5161 ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca
     5221 gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg
     5281 ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca
     5341 ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
     5401 gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     5461 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     5521 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     5581 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     5641 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     5701 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     5761 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     5821 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     5881 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     5941 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     6001 cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6061 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6121 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6181 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6241 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6301 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6361 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     6421 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6481 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6541 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta
     6601 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6661 gggggactgg aagggctaat tcactcccaa cgaagataag atctgctttt tgcttgtact
     6721 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6781 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6841 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6901 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6961 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7021 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7081 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     7141 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7201 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     7261 ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg
     7321 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7381 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7441 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7501 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7561 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7621 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7681 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7741 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7801 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7861 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7921 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7981 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8041 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8101 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     8161 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8221 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8281 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8341 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8401 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8461 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8521 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8581 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8641 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8701 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8761 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8821 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8881 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8941 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9001 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9061 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9121 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9181 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9241 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9301 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9421 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     9481 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     9541 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     9601 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     9661 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     9721 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
     9781 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     9841 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     9901 tgtaaaacga cggccagtgc caagctg
//
