LOCUS       V036326                12662 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036326
VERSION     V036326
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6756
                     /label="CFTR(NM_000492)"
                     /note="CFTR(NM_000492)"
                     /gene="CFTR"
     promoter        6821..7032
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7045..7313
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7342..7351
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7372..8034
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8104..8157
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8158..8757
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8758..9345
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9419..9652
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9724..9845
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9864..9999
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10037..10053)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10061..10077
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10085..10115)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10130..10151
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10439..11027)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11198..12058)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12059..12163)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12637..12653
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc agaggtcgcc tctggaaaag
     2341 gccagcgttg tctccaaact ttttttcagc tggaccagac caattttgag gaaaggatac
     2401 agacagcgcc tggaattgtc agacatatac caaatccctt ctgttgattc tgctgacaat
     2461 ctatctgaaa aattggaaag agaatgggat agagagctgg cttcaaagaa aaatcctaaa
     2521 ctcattaatg cccttcggcg atgttttttc tggagattta tgttctatgg aatcttttta
     2581 tatttagggg aagtcaccaa agcagtacag cctctcttac tgggaagaat catagcttcc
     2641 tatgacccgg ataacaagga ggaacgctct atcgcgattt atctaggcat aggcttatgc
     2701 cttctcttta ttgtgaggac actgctccta cacccagcca tttttggcct tcatcacatt
     2761 ggaatgcaga tgagaatagc tatgtttagt ttgatttata agaagacttt aaagctgtca
     2821 agccgtgttc tagataaaat aagtattgga caacttgtta gtctcctttc caacaacctg
     2881 aacaaatttg atgaaggact tgcattggca catttcgtgt ggatcgctcc tttgcaagtg
     2941 gcactcctca tggggctaat ctgggagttg ttacaggcgt ctgccttctg tggacttggt
     3001 ttcctgatag tccttgccct ttttcaggct gggctaggga gaatgatgat gaagtacaga
     3061 gatcagagag ctgggaagat cagtgaaaga cttgtgatta cctcagaaat gattgaaaat
     3121 atccaatctg ttaaggcata ctgctgggaa gaagcaatgg aaaaaatgat tgaaaactta
     3181 agacaaacag aactgaaact gactcggaag gcagcctatg tgagatactt caatagctca
     3241 gccttcttct tctcagggtt ctttgtggtg tttttatctg tgcttcccta tgcactaatc
     3301 aaaggaatca tcctccggaa aatattcacc accatctcat tctgcattgt tctgcgcatg
     3361 gcggtcactc ggcaatttcc ctgggctgta caaacatggt atgactctct tggagcaata
     3421 aacaaaatac aggatttctt acaaaagcaa gaatataaga cattggaata taacttaacg
     3481 actacagaag tagtgatgga gaatgtaaca gccttctggg aggagggatt tggggaatta
     3541 tttgagaaag caaaacaaaa caataacaat agaaaaactt ctaatggtga tgacagcctc
     3601 ttcttcagta atttctcact tcttggtact cctgtcctga aagatattaa tttcaagata
     3661 gaaagaggac agttgttggc ggttgctgga tccactggag caggcaagac ttcacttcta
     3721 atggtgatta tgggagaact ggagccttca gagggtaaaa ttaagcacag tggaagaatt
     3781 tcattctgtt ctcagttttc ctggattatg cctggcacca ttaaagaaaa tatcatcttt
     3841 ggtgtttcct atgatgaata tagatacaga agcgtcatca aagcatgcca actagaagag
     3901 gacatctcca agtttgcaga gaaagacaat atagttcttg gagaaggtgg aatcacactg
     3961 agtggaggtc aacgagcaag aatttcttta gcaagagcag tatacaaaga tgctgatttg
     4021 tatttattag actctccttt tggataccta gatgttttaa cagaaaaaga aatatttgaa
     4081 agctgtgtct gtaaactgat ggctaacaaa actaggattt tggtcacttc taaaatggaa
     4141 catttaaaga aagctgacaa aatattaatt ttgcatgaag gtagcagcta tttttatggg
     4201 acattttcag aactccaaaa tctacagcca gactttagct caaaactcat gggatgtgat
     4261 tctttcgacc aatttagtgc agaaagaaga aattcaatcc taactgagac cttacaccgt
     4321 ttctcattag aaggagatgc tcctgtctcc tggacagaaa caaaaaaaca atcttttaaa
     4381 cagactggag agtttgggga aaaaaggaag aattctattc tcaatccaat caactctata
     4441 cgaaaatttt ccattgtgca aaagactccc ttacaaatga atggcatcga agaggattct
     4501 gatgagcctt tagagagaag gctgtcctta gtaccagatt ctgagcaggg agaggcgata
     4561 ctgcctcgca tcagcgtgat cagcactggc cccacgcttc aggcacgaag gaggcagtct
     4621 gtcctgaacc tgatgacaca ctcagttaac caaggtcaga acattcaccg aaagacaaca
     4681 gcatccacac gaaaagtgtc actggcccct caggcaaact tgactgaact ggatatatat
     4741 tcaagaaggt tatctcaaga aactggcttg gaaataagtg aagaaattaa cgaagaagac
     4801 ttaaaggagt gcttttttga tgatatggag agcataccag cagtgactac atggaacaca
     4861 taccttcgat atattactgt ccacaagagc ttaatttttg tgctaatttg gtgcttagta
     4921 atttttctgg cagaggtggc tgcttctttg gttgtgctgt ggctccttgg aaacactcct
     4981 cttcaagaca aagggaatag tactcatagt agaaataaca gctatgcagt gattatcacc
     5041 agcaccagtt cgtattatgt gttttacatt tacgtgggag tagccgacac tttgcttgct
     5101 atgggattct tcagaggtct accactggtg catactctaa tcacagtgtc gaaaatttta
     5161 caccacaaaa tgttacattc tgttcttcaa gcacctatgt caaccctcaa cacgttgaaa
     5221 gcaggtggga ttcttaatag attctccaaa gatatagcaa ttttggatga ccttctgcct
     5281 cttaccatat ttgacttcat ccagttgtta ttaattgtga ttggagctat agcagttgtc
     5341 gcagttttac aaccctacat ctttgttgca acagtgccag tgatagtggc ttttattatg
     5401 ttgagagcat atttcctcca aacctcacag caactcaaac aactggaatc tgaaggcagg
     5461 agtccaattt tcactcatct tgttacaagc ttaaaaggac tatggacact tcgtgccttc
     5521 ggacggcagc cttactttga aactctgttc cacaaagctc tgaatttaca tactgccaac
     5581 tggttcttgt acctgtcaac actgcgctgg ttccaaatga gaatagaaat gatttttgtc
     5641 atcttcttca ttgctgttac cttcatttcc attttaacaa caggagaagg agaaggaaga
     5701 gttggtatta tcctgacttt agccatgaat atcatgagta cattgcagtg ggctgtaaac
     5761 tccagcatag atgtggatag cttgatgcga tctgtgagcc gagtctttaa gttcattgac
     5821 atgccaacag aaggtaaacc taccaagtca accaaaccat acaagaatgg ccaactctcg
     5881 aaagttatga ttattgagaa ttcacacgtg aagaaagatg acatctggcc ctcagggggc
     5941 caaatgactg tcaaagatct cacagcaaaa tacacagaag gtggaaatgc catattagag
     6001 aacatttcct tctcaataag tcctggccag agggtgggcc tcttgggaag aactggatca
     6061 gggaagagta ctttgttatc agcttttttg agactactga acactgaagg agaaatccag
     6121 atcgatggtg tgtcttggga ttcaataact ttgcaacagt ggaggaaagc ctttggagtg
     6181 ataccacaga aagtatttat tttttctgga acatttagaa aaaacttgga tccctatgaa
     6241 cagtggagtg atcaagaaat atggaaagtt gcagatgagg ttgggctcag atctgtgata
     6301 gaacagtttc ctgggaagct tgactttgtc cttgtggatg ggggctgtgt cctaagccat
     6361 ggccacaagc agttgatgtg cttggctaga tctgttctca gtaaggcgaa gatcttgctg
     6421 cttgatgaac ccagtgctca tttggatcca gtaacatacc aaataattag aagaactcta
     6481 aaacaagcat ttgctgattg cacagtaatt ctctgtgaac acaggataga agcaatgctg
     6541 gaatgccaac aatttttggt catagaagag aacaaagtgc ggcagtacga ttccatccag
     6601 aaactgctga acgagaggag cctcttccgg caagccatca gcccctccga cagggtgaag
     6661 ctctttcccc accggaactc aagcaagtgc aagtctaagc cccagattgc tgctctgaaa
     6721 gaggagacag aagaagaggt gcaagataca aggctttagg gatccctgcg atatccagcg
     6781 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     6841 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     6901 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     6961 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     7021 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     7081 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     7141 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     7201 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     7261 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     7321 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg
     7381 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc
     7441 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc
     7501 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc
     7561 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc
     7621 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc
     7681 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac
     7741 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc
     7801 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc
     7861 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc
     7921 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc
     7981 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc
     8041 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct
     8101 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     8161 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     8221 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     8281 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     8341 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     8401 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     8461 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     8521 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     8581 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     8641 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     8701 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     8761 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8821 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8881 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8941 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     9001 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     9061 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     9121 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     9181 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     9241 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc
     9301 cttcgccctc agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga
     9361 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     9421 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc
     9481 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     9541 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     9601 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     9661 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     9721 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9781 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9841 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc
     9901 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     9961 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc
    10021 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    10081 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    10141 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    10201 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    10261 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    10321 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    10381 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    10441 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    10501 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    10561 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    10621 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    10681 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10741 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    10801 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10861 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10921 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10981 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    11041 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    11101 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    11161 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    11221 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    11281 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    11341 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    11401 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    11461 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    11521 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    11581 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    11641 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    11701 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    11761 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    11821 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11881 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11941 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    12001 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    12061 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    12121 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    12181 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    12241 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    12301 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    12361 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
    12421 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    12481 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    12541 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    12601 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
    12661 tg
//
