LOCUS V037741 8042 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037741 VERSION V037741 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..2874 /codon_start=1 /product="human Rho family small GTPase Cdc42" /transl_table=1 /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM IGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK GLKNVFDEAILAALEPPEPKKSRRCVLL" /label="Cdc42" CDS 2881..2907 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2911..2937 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2941..2967 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 3012..3223 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3236..3504 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 3531..4130 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4137..4725 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 4799..5032 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5104..5225 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5244..5379 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(5417..5433) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5441..5457 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5465..5495) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5510..5531 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5819..6407) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6578..7438) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7439..7543) /gene="bla" /label="AmpR promoter" primer_bind 8017..8033 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgcagaca attaagtgtg ttgttgtggg cgatggtgct 2341 gttggtaaaa catgtctcct gatatcctac acaacaaaca aatttccatc ggaatatgta 2401 ccgactgttt ttgacaacta tgcagtcaca gttatgattg gtggagaacc atatactctt 2461 ggactttttg atactgcagg gcaagaggat tatgacagat tacgaccgct gagttatcca 2521 caaacagatg tatttctagt ctgtttttca gtggtctctc catcttcatt tgaaaacgtg 2581 aaagaaaagt gggtgcctga gataactcac cactgtccaa agactccttt cttgcttgtt 2641 gggactcaaa ttgatctcag agatgacccc tctactattg agaaacttgc caagaacaaa 2701 cagaagccta tcactccaga gactgctgaa aagctggccc gtgacctgaa ggctgtcaag 2761 tatgtggagt gttctgcact tacacagaaa ggcctaaaga atgtatttga cgaagcaata 2821 ttggctgccc tggagcctcc agaaccgaag aagagccgca ggtgtgtgct gctaggatcc 2881 tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga ttatgctagc 2941 tatccttatg atgtcccaga ctacgcatag gcggccgcga aggatctgcg atcgctccgg 3001 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 3061 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 3121 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 3181 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc 3241 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag 3301 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag 3361 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact 3421 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt 3481 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag atgaccgagt 3541 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg 3601 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg 3661 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 3721 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 3781 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 3841 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 3901 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 3961 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 4021 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 4081 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc 4141 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 4201 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 4261 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 4321 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 4381 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 4441 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 4501 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct 4561 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 4621 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 4681 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga 4741 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 4801 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc 4861 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 4921 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 4981 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 5041 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 5101 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 5161 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 5221 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 5281 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 5341 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 5401 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 5461 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 5521 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 5581 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 5641 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 5701 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 5761 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 5821 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 5881 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 5941 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 6001 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 6061 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 6121 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 6181 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 6241 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 6301 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 6361 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 6421 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 6481 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 6541 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 6601 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 6661 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 6721 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 6781 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 6841 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 6901 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 6961 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 7021 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 7081 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 7141 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 7201 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 7261 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 7321 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 7381 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 7441 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 7501 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 7561 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 7621 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 7681 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 7741 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 7801 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 7861 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 7921 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 7981 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 8041 tg //