LOCUS       V020161                 9918 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020161
VERSION     V020161
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4837
                     /label="CD22(NM_001771)"
                     /note="CD22(NM_001771)"
                     /gene="CD22"
     promoter        4888..5099
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5112..5380
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5407..6006
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6013..6601
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6675..6908
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6980..7101
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7120..7255
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7293..7309)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7317..7333
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7341..7371)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7386..7407
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7695..8283)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8454..9314)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9315..9419)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9893..9909
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgc atctcctcgg cccctggctc ctgctcctgg ttctagaata
     2341 cttggctttc tctgactcaa gtaaatgggt ttttgagcac cctgaaaccc tctacgcctg
     2401 ggagggggcc tgcgtctgga tcccctgcac ctacagagcc ctagatggtg acctggaaag
     2461 cttcatcctg ttccacaatc ctgagtataa caagaacacc tcgaagtttg atgggacaag
     2521 actctatgaa agcacaaagg atgggaaggt tccttctgag cagaaaaggg tgcaattcct
     2581 gggagacaag aataagaact gcacactgag tatccacccg gtgcacctca atgacagtgg
     2641 tcagctgggg ctgaggatgg agtccaagac tgagaaatgg atggaacgaa tacacctcaa
     2701 tgtctctgaa aggccttttc cacctcatat ccagctccct ccagaaattc aagagtccca
     2761 ggaagtcact ctgacctgct tgctgaattt ctcctgctat gggtatccga tccaattgca
     2821 gtggctccta gagggggttc caatgaggca ggctgctgtc acctcgacct ccttgaccat
     2881 caagtctgtc ttcacccgga gcgagctcaa gttctcccca cagtggagtc accatgggaa
     2941 gattgtgacc tgccagcttc aggatgcaga tgggaagttc ctctccaatg acacggtgca
     3001 gctgaacgtg aagcacaccc cgaagttgga gatcaaggtc actcccagtg atgccatagt
     3061 gagggagggg gactctgtga ccatgacctg cgaggtcagc agcagcaacc cggagtacac
     3121 gacggtatcc tggctcaagg atgggacctc gctgaagaag cagaatacat tcacgctaaa
     3181 cctgcgcgaa gtgaccaagg accagagtgg gaagtactgc tgtcaggtct ccaatgacgt
     3241 gggcccggga aggtcggaag aagtgttcct gcaagtgcag tatgccccgg aaccttccac
     3301 ggttcagatc ctccactcac cggctgtgga gggaagtcaa gtcgagtttc tttgcatgtc
     3361 actggccaat cctcttccaa caaattacac gtggtaccac aatgggaaag aaatgcaggg
     3421 aaggacagag gagaaagtcc acatcccaaa gatcctcccc tggcacgctg ggacttattc
     3481 ctgtgtggca gaaaacattc ttggtactgg acagaggggc ccgggagctg agctggatgt
     3541 ccagtatcct cccaagaagg tgaccacagt gattcaaaac cccatgccga ttcgagaagg
     3601 agacacagtg accctttcct gtaactacaa ttccagtaac cccagtgtta cccggtatga
     3661 atggaaaccc catggcgcct gggaggagcc atcgcttggg gtgctgaaga tccaaaacgt
     3721 tggctgggac aacacaacca tcgcctgcgc agcttgtaat agttggtgct cgtgggcctc
     3781 ccctgtcgcc ctgaatgtcc agtatgcccc ccgagacgtg agggtccgga aaatcaagcc
     3841 cctttccgag attcactctg gaaactcggt cagcctccaa tgtgacttct caagcagcca
     3901 ccccaaagaa gtccagttct tctgggagaa aaatggcagg cttctgggga aagaaagcca
     3961 gctgaatttt gactccatct ccccagaaga tgctgggagt tacagctgct gggtgaacaa
     4021 ctccatagga cagacagcgt ccaaggcctg gacacttgaa gtgctgtatg cacccaggag
     4081 gctgcgtgtg tccatgagcc cgggggacca agtgatggag gggaagagtg caaccctgac
     4141 ctgtgagagc gacgccaacc ctcccgtctc ccactacacc tggtttgact ggaataacca
     4201 aagcctcccc taccacagcc agaagctgag attggagccg gtgaaggtcc agcactcggg
     4261 tgcctactgg tgccagggga ccaacagtgt gggcaagggc cgttcgcctc tcagcaccct
     4321 caccgtctac tatagcccgg agaccatcgg caggcgagtg gctgtgggac tcgggtcctg
     4381 cctcgccatc ctcatcctgg caatctgtgg gctcaagctc cagcgacgtt ggaagaggac
     4441 acagagccag caggggcttc aggagaattc cagcggccag agcttctttg tgaggaataa
     4501 aaaggttaga agggcccccc tctctgaagg cccccactcc ctgggatgct acaatccaat
     4561 gatggaagat ggcattagct acaccaccct gcgctttccc gagatgaaca taccacgaac
     4621 tggagatgca gagtcctcag agatgcagag acctcccccg gactgcgatg acacggtcac
     4681 ttattcagca ttgcacaagc gccaagtggg cgactatgag aacgtcattc cagattttcc
     4741 agaagatgag gggattcatt actcagagct gatccagttt ggggtcgggg agcggcctca
     4801 ggcacaagaa aatgtggact atgtgatcct caaacattga ggatccgcgg ccgcgaagga
     4861 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     4921 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact
     4981 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     5041 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc
     5101 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc
     5161 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt
     5221 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt
     5281 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct
     5341 acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta
     5401 cgctagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg
     5461 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac
     5521 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5581 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     5641 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     5701 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     5761 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     5821 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     5881 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     5941 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6001 gcctgagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6061 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6121 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6181 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6241 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6301 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6361 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     6421 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6481 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6541 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6601 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6661 agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc
     6721 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6781 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6841 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6901 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     6961 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7021 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7081 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7141 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7201 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     7261 ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7321 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7381 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7441 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7501 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7561 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7621 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7681 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7741 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7801 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7861 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7921 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7981 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8041 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8101 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     8161 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8221 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8281 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8341 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8401 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8461 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8521 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8581 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8641 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8701 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8761 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8821 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8881 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8941 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9001 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9061 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9121 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9181 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9241 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9301 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9361 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9421 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     9481 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     9541 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     9601 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     9661 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     9721 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     9781 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     9841 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     9901 acggccagtg ccaagctg
//
