LOCUS       V044111                12473 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044111
VERSION     V044111
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2287..2352
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             2371..7398
                     /label="C5(NM_001735)"
                     /note="C5(NM_001735)"
                     /gene="C5"
     promoter        7443..7654
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7667..7935
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             7962..8561
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8568..9156
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9230..9463
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9535..9656
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9675..9810
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9848..9864)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9872..9888
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9896..9926)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9941..9962
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10250..10838)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11009..11869)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11870..11974)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12448..12464
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agaatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
     2341 gacgatgaca agtctagaga attcggatcc atgggccttt tgggaatact ttgtttttta
     2401 atcttcctgg ggaaaacctg gggacaggag caaacatatg tcatttcagc accaaaaata
     2461 ttccgtgttg gagcatctga aaatattgtg attcaagttt atggatacac tgaagcattt
     2521 gatgcaacaa tctctattaa aagttatcct gataaaaaat ttagttactc ctcaggccat
     2581 gttcatttat cctcagagaa taaattccaa aactctgcaa tcttaacaat acaaccaaaa
     2641 caattgcctg gaggacaaaa cccagtttct tatgtgtatt tggaagttgt atcaaagcat
     2701 ttttcaaaat caaaaagaat gccaataacc tatgacaatg gatttctctt cattcataca
     2761 gacaaacctg tttatactcc agaccagtca gtaaaagtta gagtttattc gttgaatgac
     2821 gacttgaagc cagccaaaag agaaactgtc ttaactttca tagatcctga aggatcagaa
     2881 gttgacatgg tagaagaaat tgatcatatt ggaattatct cttttcctga cttcaagatt
     2941 ccgtctaatc ctagatatgg tatgtggacg atcaaggcta aatataaaga ggacttttca
     3001 acaactggaa ccgcatattt tgaagttaaa gaatatgtct tgccacattt ttctgtctca
     3061 atcgagccag aatataattt cattggttac aagaacttta agaattttga aattactata
     3121 aaagcaagat atttttataa taaagtagtc actgaggctg acgtttatat cacatttgga
     3181 ataagagaag acttaaaaga tgatcaaaaa gaaatgatgc aaacagcaat gcaaaacaca
     3241 atgttgataa atggaattgc tcaagtcaca tttgattctg aaacagcagt caaagaactg
     3301 tcatactaca gtttagaaga tttaaacaac aagtaccttt atattgctgt aacagtcata
     3361 gagtctacag gtggattttc tgaagaggca gaaatacctg gcatcaaata tgtcctctct
     3421 ccctacaaac tgaatttggt tgctactcct cttttcctga agcctgggat tccatatccc
     3481 atcaaggtgc aggttaaaga ttcgcttgac cagttggtag gaggagtccc agtaacactg
     3541 aatgcacaaa caattgatgt aaaccaagag acatctgact tggatccaag caaaagtgta
     3601 acacgtgttg atgatggagt agcttccttt gtgcttaatc tcccatctgg agtgacggtg
     3661 ctggagttta atgtcaaaac tgatgctcca gatcttccag aagaaaatca ggccagggaa
     3721 ggttaccgag caatagcata ctcatctctc agccaaagtt acctttatat tgattggact
     3781 gataaccata aggctttgct agtgggagaa catctgaata ttattgttac ccccaaaagc
     3841 ccatatattg acaaaataac tcactataat tacttgattt tatccaaggg caaaattatc
     3901 cactttggca cgagggagaa attttcagat gcatcttatc aaagtataaa cattccagta
     3961 acacagaaca tggttccttc atcccgactt ctggtctatt acatcgtcac aggagaacag
     4021 acagcagaat tagtgtctga ttcagtctgg ttaaatattg aagaaaaatg tggcaaccag
     4081 ctccaggttc atctgtctcc tgatgcagat gcatattctc caggccaaac tgtgtctctt
     4141 aatatggcaa ctggaatgga ttcctgggtg gcattagcag cagtggacag tgctgtgtat
     4201 ggagtccaaa gaggagccaa aaagcccttg gaaagagtat ttcaattctt agagaagagt
     4261 gatctgggct gtggggcagg tggtggcctc aacaatgcca atgtgttcca cctagctgga
     4321 cttaccttcc tcactaatgc aaatgcagat gactcccaag aaaatgatga accttgtaaa
     4381 gaaattctca ggccaagaag aacgctgcaa aagaagatag aagaaatagc tgctaaatat
     4441 aaacattcag tagtgaagaa atgttgttac gatggagcct gcgttaataa tgatgaaacc
     4501 tgtgagcagc gagctgcacg gattagttta gggccaagat gcatcaaagc tttcactgaa
     4561 tgttgtgtcg tcgcaagcca gctccgtgct aatatctctc ataaagacat gcaattggga
     4621 aggctacaca tgaagaccct gttaccagta agcaagccag aaattcggag ttattttcca
     4681 gaaagctggt tgtgggaagt tcatcttgtt cccagaagaa aacagttgca gtttgcccta
     4741 cctgattctc taaccacctg ggaaattcaa ggcgttggca tttcaaacac tggtatatgt
     4801 gttgctgata ctgtcaaggc aaaggtgttc aaagatgtct tcctggaaat gaatatacca
     4861 tattctgttg tacgaggaga acagatccaa ttgaaaggaa ctgtttacaa ctataggact
     4921 tctgggatgc agttctgtgt taaaatgtct gctgtggagg gaatctgcac ttcggaaagc
     4981 ccagtcattg atcatcaggg cacaaagtcc tccaaatgtg tgcgccagaa agtagagggc
     5041 tcctccagtc acttggtgac attcactgtg cttcctctgg aaattggcct tcacaacatc
     5101 aatttttcac tggagacttg gtttggaaaa gaaatcttag taaaaacatt acgagtggtg
     5161 ccagaaggtg tcaaaaggga aagctattct ggtgttactt tggatcctag gggtatttat
     5221 ggtaccatta gcagacgaaa ggagttccca tacaggatac ccttagattt ggtccccaaa
     5281 acagaaatca aaaggatttt gagtgtaaaa ggactgcttg taggtgagat cttgtctgca
     5341 gttctaagtc aggaaggcat caatatccta acccacctcc ccaaagggag tgcagaggcg
     5401 gagctgatga gcgttgtccc agtattctat gtttttcact acctggaaac aggaaatcat
     5461 tggaacattt ttcattctga cccattaatt gaaaagcaga aactgaagaa aaaattaaaa
     5521 gaagggatgt tgagcattat gtcctacaga aatgctgact actcttacag tgtgtggaag
     5581 ggtggaagtg ctagcacttg gttaacagct tttgctttaa gagtacttgg acaagtaaat
     5641 aaatacgtag agcagaacca aaattcaatt tgtaattctt tattgtggct agttgagaat
     5701 tatcaattag ataatggatc tttcaaggaa aattcacagt atcaaccaat aaaattacag
     5761 ggtaccttgc ctgttgaagc ccgagagaac agcttatatc ttacagcctt tactgtgatt
     5821 ggaattagaa aggctttcga tatatgcccc ctggtgaaaa tcgacacagc tctaattaaa
     5881 gctgacaact ttctgcttga aaatacactg ccagcccaga gcacctttac attggccatt
     5941 tctgcgtatg ctctttccct gggagataaa actcacccac agtttcgttc aattgtttca
     6001 gctttgaaga gagaagcttt ggttaaaggt aatccaccca tttatcgttt ttggaaagac
     6061 aatcttcagc ataaagacag ctctgtacct aacactggta cggcacgtat ggtagaaaca
     6121 actgcctatg ctttactcac cagtctgaac ttgaaagata taaattatgt taacccagtc
     6181 atcaaatggc tatcagaaga gcagaggtat ggaggtggct tttattcaac ccaggacaca
     6241 atcaatgcca ttgagggcct gacggaatat tcactcctgg ttaaacaact ccgcttgagt
     6301 atggacatcg atgtttctta caagcataaa ggtgccttac ataattataa aatgacagac
     6361 aagaatttcc ttgggaggcc agtagaggtg cttctcaatg atgacctcat tgtcagtaca
     6421 ggatttggca gtggcttggc tacagtacat gtaacaactg tagttcacaa aaccagtacc
     6481 tctgaggaag tttgcagctt ttatttgaaa atcgatactc aggatattga agcatcccac
     6541 tacagaggct acggaaactc tgattacaaa cgcatagtag catgtgccag ctacaagccc
     6601 agcagggaag aatcatcatc tggatcctct catgcggtga tggacatctc cttgcctact
     6661 ggaatcagtg caaatgaaga agacttaaaa gcccttgtgg aaggggtgga tcaactattc
     6721 actgattacc aaatcaaaga tggacatgtt attctgcaac tgaattcgat tccctccagt
     6781 gatttccttt gtgtacgatt ccggatattt gaactctttg aagttgggtt tctcagtcct
     6841 gccactttca cagtgtacga ataccacaga ccagataaac agtgtaccat gttttatagc
     6901 acttccaata tcaaaattca gaaagtctgt gaaggagccg cgtgcaagtg tgtagaagct
     6961 gattgtgggc aaatgcagga agaattggat ctgacaatct ctgcagagac aagaaaacaa
     7021 acagcatgta aaccagagat tgcatatgct tataaagtta gcatcacatc catcactgta
     7081 gaaaatgttt ttgtcaagta caaggcaacc cttctggata tctacaaaac tggggaagct
     7141 gttgctgaga aagactctga gattaccttc attaaaaagg taacctgtac taacgctgag
     7201 ctggtaaaag gaagacagta cttaattatg ggtaaagaag ccctccagat aaaatacaat
     7261 ttcagtttca ggtacatcta ccctttagat tccttgacct ggattgaata ctggcctaga
     7321 gacacaacat gttcatcgtg tcaagcattt ttagctaatt tagatgaatt tgccgaagat
     7381 atctttttaa atggatgcta agcggccgcg aaggatctgc gatcgctccg gtgcccgtca
     7441 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     7501 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     7561 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     7621 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc
     7681 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct
     7741 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
     7801 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
     7861 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
     7921 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gatgaccgag tacaagccca
     7981 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     8041 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     8101 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     8161 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     8221 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     8281 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     8341 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     8401 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     8461 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     8521 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg agtcgacaat caacctctgg
     8581 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     8641 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     8701 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     8761 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     8821 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     8881 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     8941 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     9001 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     9061 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     9121 agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag accaatgact
     9181 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     9241 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac
     9301 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9361 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9421 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     9481 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     9541 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9601 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9661 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9721 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     9781 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt
     9841 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     9901 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     9961 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    10021 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    10081 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    10141 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    10201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    10261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    10321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    10441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    10561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    10621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    10681 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    10741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    10801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    10861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    10921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10981 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    11041 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    11101 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    11161 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    11221 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    11281 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    11341 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    11401 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    11461 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    11521 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    11581 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    11641 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    11701 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    11761 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    11821 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    11881 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    11941 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    12001 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    12061 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    12121 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    12181 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
    12241 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    12301 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    12361 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    12421 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
