LOCUS       V036487                 9406 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036487
VERSION     V036487
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4455
                     /label="Mecom(NM_007963)"
                     /note="Mecom(NM_007963)"
                     /gene="Mecom"
     CDS             4462..4527
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4571..4782
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4795..5063
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5096..5494
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    5501..6089
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6163..6396
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6468..6589
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6608..6743
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(6781..6797)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6805..6821
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6829..6859)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6874..6895
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7183..7771)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7942..8802)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8803..8907)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9381..9397
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgaagagt gaagaggacc cgcacgaacc catggcgcct
     2341 gacatccacg aagaacggca gcaccgctgt gaggactgtg accagctctt tgaatccaag
     2401 gcagagctag ccgatcacca gaagttccca tgcagcacac ctcactcggc cttctccatg
     2461 gtggaggagg acttgcaaca aaacctggag agtgagagcg atctccgaga gatccatggc
     2521 aaccaggact gtaaggaatg tgaccgagtt ttccccgatc tgcaaagctt ggagaagcac
     2581 atgctgtcac atactgagga gagggaatac aagtgtgatc agtgtcccaa ggcatttaac
     2641 tggaagtcca atttaattcg ccaccagatg tcacatgaca gtggaaagca ctatgagtgt
     2701 gaaaactgtg ccaaggtttt cacggaccct agcaaccttc agcgacacat tcgatctcag
     2761 catgttggtg cccgggctca tgcttgcccc gagtgtggta aaacatttgc cacttcgtca
     2821 ggcctcaaac agcacaagca catccacagc agtgtgaagc cctttatctc attctctcaa
     2881 tcaatgtacc catttcctga tagagacttg aggtcgttac ctttgaaaat ggagccccaa
     2941 tcaccaagtg aagttaagaa actgcagaag ggaagctctg agtccccttt tgacctcacc
     3001 actaagagaa aggatgagaa gcccttgact tcaggcccct cgaagccttc aggaacacca
     3061 gccacaagcc aagaccagcc cctggatcta agtatgggca gtaggggtag agccagtggg
     3121 acaaagttga ctgagcctcg aaaaaaccat gtgtttgggg aaaagaaagg aagcaacatg
     3181 gatactaggc catcttcaga tggctccttg cagcatgcca gacccactcc cttcttcatg
     3241 gaccccattt atagagtaga gaaaagaaag ttaactgacc cgcttgaagc tttgaaagaa
     3301 aaatacttga gaccttctcc aggattcttg tttcacccgc aaatgtcagc aattgagaac
     3361 atggcagaaa agctggaaag cttcagcgcc ctcaaacctg aggccagcga gctcctgcag
     3421 tccgtgccct ccatgttcag cttccgagct cctcccaaca ccctgccaga gaacctgctg
     3481 cggaagggga aagagcgcta cacctgcagg tactgtggca agatatttcc aaggtctgcg
     3541 aacctaacac ggcacttgag aacccacaca ggagagcaac cttacagatg caaatactgt
     3601 gatagatcat tcagcatttc ttccaacctg cagcgacatg tgcgcaacat ccacaacaag
     3661 gagaagccat ttaagtgtca tttatgtgac agatgttttg gtcaacaaac caatcttgac
     3721 agacacctga agaaacatga gaacggcaac atgtctggga cggcaacgtc ctcgcctcac
     3781 tcagagctag aaagcgcagg cgcaatcctg gatgacaaag aagatgcgta ctttacagag
     3841 atccgcaatt tcatcgggaa cagcaaccat ggtagccagt ctcctcggaa catggaagag
     3901 aggatgaatg gcagtcactt caaggataaa aaggctttgg caaccagcca aaattcagat
     3961 ttattggatg atgaagaagt agaagatgag gtgttgttgg atgaggagga tgaagacaat
     4021 gatattcctg gaaagcccag aaaggagcta ggggtgactc gtttagacga ggagatcccg
     4081 gaggatgact acgaagaagc tggtgccctg gagatgagct gtaaggcgtc cccggtgagg
     4141 tataaagagg aagactataa atctggcctt tctgctctag atcacataag gcacttcaca
     4201 gatagcctca aaatgaggga aatggaagag aatcaataca ctgacgctga gctgtcctcc
     4261 attagttctt ctcatgtgcc agaggagctt aaacagacgt tacacagaaa gtccaaatca
     4321 caggcatatg ctatgatgtt gtcactgtct gacaaggatt ccctccatcc cacctcccac
     4381 agttcctcca acgtgtggca cagcatggca agggctgcag cagaatccag tgccatccag
     4441 tccataagcc atgtaggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     4501 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     4561 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     4621 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     4681 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     4741 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     4801 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     4861 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     4921 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     4981 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     5041 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctagg ccaccatggc
     5101 caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
     5161 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
     5221 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
     5281 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
     5341 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
     5401 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
     5461 attgctgccc tctggttatg tgtgggaggg ctaagtcgac aatcaacctc tggattacaa
     5521 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     5581 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     5641 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     5701 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     5761 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     5821 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     5881 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     5941 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6001 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6061 tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg
     6121 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     6181 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     6241 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6301 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6361 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     6421 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     6481 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6541 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     6601 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6661 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6721 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg
     6781 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6841 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6901 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6961 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7021 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7081 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7141 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7201 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7261 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7321 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7381 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7441 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7501 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7561 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7621 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7681 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7741 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7801 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7861 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7921 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7981 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8041 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8101 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8161 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8221 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8281 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8341 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8401 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8461 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8521 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8581 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8641 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8701 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8761 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8821 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8881 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     8941 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     9001 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     9061 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     9121 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     9181 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     9241 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     9301 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     9361 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
