LOCUS V036487 9406 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V036487 VERSION V036487 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4455 /label="Mecom(NM_007963)" /note="Mecom(NM_007963)" /gene="Mecom" CDS 4462..4527 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4571..4782 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4795..5063 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 5096..5494 /codon_start=1 /gene="Aspergillus terreus BSD" /note="confers resistance to blasticidin" /product="blasticidin S deaminase" /transl_table=1 /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG*" /label="BSD" misc_feature 5501..6089 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6163..6396 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6468..6589 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6608..6743 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6781..6797) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6805..6821 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6829..6859) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6874..6895 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7183..7771) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7942..8802) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8803..8907) /gene="bla" /label="AmpR promoter" primer_bind 9381..9397 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgaagagt gaagaggacc cgcacgaacc catggcgcct 2341 gacatccacg aagaacggca gcaccgctgt gaggactgtg accagctctt tgaatccaag 2401 gcagagctag ccgatcacca gaagttccca tgcagcacac ctcactcggc cttctccatg 2461 gtggaggagg acttgcaaca aaacctggag agtgagagcg atctccgaga gatccatggc 2521 aaccaggact gtaaggaatg tgaccgagtt ttccccgatc tgcaaagctt ggagaagcac 2581 atgctgtcac atactgagga gagggaatac aagtgtgatc agtgtcccaa ggcatttaac 2641 tggaagtcca atttaattcg ccaccagatg tcacatgaca gtggaaagca ctatgagtgt 2701 gaaaactgtg ccaaggtttt cacggaccct agcaaccttc agcgacacat tcgatctcag 2761 catgttggtg cccgggctca tgcttgcccc gagtgtggta aaacatttgc cacttcgtca 2821 ggcctcaaac agcacaagca catccacagc agtgtgaagc cctttatctc attctctcaa 2881 tcaatgtacc catttcctga tagagacttg aggtcgttac ctttgaaaat ggagccccaa 2941 tcaccaagtg aagttaagaa actgcagaag ggaagctctg agtccccttt tgacctcacc 3001 actaagagaa aggatgagaa gcccttgact tcaggcccct cgaagccttc aggaacacca 3061 gccacaagcc aagaccagcc cctggatcta agtatgggca gtaggggtag agccagtggg 3121 acaaagttga ctgagcctcg aaaaaaccat gtgtttgggg aaaagaaagg aagcaacatg 3181 gatactaggc catcttcaga tggctccttg cagcatgcca gacccactcc cttcttcatg 3241 gaccccattt atagagtaga gaaaagaaag ttaactgacc cgcttgaagc tttgaaagaa 3301 aaatacttga gaccttctcc aggattcttg tttcacccgc aaatgtcagc aattgagaac 3361 atggcagaaa agctggaaag cttcagcgcc ctcaaacctg aggccagcga gctcctgcag 3421 tccgtgccct ccatgttcag cttccgagct cctcccaaca ccctgccaga gaacctgctg 3481 cggaagggga aagagcgcta cacctgcagg tactgtggca agatatttcc aaggtctgcg 3541 aacctaacac ggcacttgag aacccacaca ggagagcaac cttacagatg caaatactgt 3601 gatagatcat tcagcatttc ttccaacctg cagcgacatg tgcgcaacat ccacaacaag 3661 gagaagccat ttaagtgtca tttatgtgac agatgttttg gtcaacaaac caatcttgac 3721 agacacctga agaaacatga gaacggcaac atgtctggga cggcaacgtc ctcgcctcac 3781 tcagagctag aaagcgcagg cgcaatcctg gatgacaaag aagatgcgta ctttacagag 3841 atccgcaatt tcatcgggaa cagcaaccat ggtagccagt ctcctcggaa catggaagag 3901 aggatgaatg gcagtcactt caaggataaa aaggctttgg caaccagcca aaattcagat 3961 ttattggatg atgaagaagt agaagatgag gtgttgttgg atgaggagga tgaagacaat 4021 gatattcctg gaaagcccag aaaggagcta ggggtgactc gtttagacga ggagatcccg 4081 gaggatgact acgaagaagc tggtgccctg gagatgagct gtaaggcgtc cccggtgagg 4141 tataaagagg aagactataa atctggcctt tctgctctag atcacataag gcacttcaca 4201 gatagcctca aaatgaggga aatggaagag aatcaataca ctgacgctga gctgtcctcc 4261 attagttctt ctcatgtgcc agaggagctt aaacagacgt tacacagaaa gtccaaatca 4321 caggcatatg ctatgatgtt gtcactgtct gacaaggatt ccctccatcc cacctcccac 4381 agttcctcca acgtgtggca cagcatggca agggctgcag cagaatccag tgccatccag 4441 tccataagcc atgtaggatc cgactacaaa gaccatgacg gtgattataa agatcatgac 4501 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt 4561 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 4621 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 4681 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 4741 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 4801 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 4861 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 4921 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 4981 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 5041 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctagg ccaccatggc 5101 caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag 5161 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat 5221 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct 5281 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga 5341 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc 5401 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga 5461 attgctgccc tctggttatg tgtgggaggg ctaagtcgac aatcaacctc tggattacaa 5521 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 5581 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 5641 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 5701 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 5761 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 5821 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 5881 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat 5941 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 6001 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 6061 tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg 6121 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 6181 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 6241 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 6301 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 6361 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 6421 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 6481 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 6541 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 6601 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 6661 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 6721 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg 6781 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 6841 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 6901 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 6961 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 7021 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 7081 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 7141 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 7201 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 7261 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 7321 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 7381 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 7441 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 7501 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 7561 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 7621 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 7681 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 7741 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 7801 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 7861 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 7921 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 7981 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 8041 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 8101 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 8161 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 8221 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 8281 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 8341 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 8401 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 8461 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 8521 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 8581 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 8641 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 8701 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 8761 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 8821 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 8881 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 8941 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 9001 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 9061 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 9121 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 9181 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 9241 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 9301 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 9361 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg //