LOCUS V046624 7934 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046624 VERSION V046624 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3048 /label="Fcgr4(NM_144559)" /note="Fcgr4(NM_144559)" /gene="Fcgr4" promoter 3099..3310 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3323..3591 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 3624..4022 /codon_start=1 /gene="Aspergillus terreus BSD" /note="confers resistance to blasticidin" /product="blasticidin S deaminase" /transl_table=1 /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG*" /label="BSD" misc_feature 4029..4617 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 4691..4924 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 4996..5117 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5136..5271 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(5309..5325) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5333..5349 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5357..5387) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5402..5423 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5711..6299) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6470..7330) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7331..7435) /gene="bla" /label="AmpR promoter" primer_bind 7909..7925 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgtggcag ctactactac caacagctct ggtacttaca 2341 gctttctctg gcattcaagc tggtctccaa aaggctgtgg tgaacctaga ccccaagtgg 2401 gtcagggtgc ttgaggaaga cagcgtgacc ctcagatgcc aaggcacttt ctcccccgag 2461 gacaattcta tcaagtggtt ccataacgaa agcctcatcc cacaccagga tgccaactat 2521 gtcatccaaa gtgccagagt taaggacagt ggaatgtaca ggtgccagac agccctctcc 2581 acgatcagtg acccagtgca actagaggtc catatgggct ggctattgct tcagaccact 2641 aagtggctgt tccaggaggg ggaccccatt catctgagat gccacagttg gcaaaacaga 2701 cctgtacgga aggtcaccta tttacagaac ggcaaaggca agaagtattt ccatgaaaat 2761 tctgaattac tcattccaaa agctacacac aatgacagtg gctcctactt ctgcagaggg 2821 ctcattggac acaacaacaa atcttcagca tcctttcgta taagcctagg cgatccaggg 2881 tctccatcca tgtttccacc gtggcatcaa atcacattct gcctgctgat aggactcttg 2941 tttgcaatag acacagtgct gtatttctct gtgcggaggg gtcttcaaag tcctgtggct 3001 gactatgagg aacccaagat tcaatggagc aaggaacctc aggacaagtg aggatccgcg 3061 gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac 3121 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg 3181 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg 3241 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc 3301 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac 3361 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc 3421 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc 3481 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct 3541 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg 3601 accggcgcct acgctaggcc accatggcca agcctttgtc tcaagaagaa tccaccctca 3661 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca 3721 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg 3781 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc 3841 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt 3901 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg 3961 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct 4021 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 4081 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 4141 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 4201 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 4261 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 4321 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 4381 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt 4441 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 4501 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 4561 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 4621 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 4681 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt 4741 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 4801 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 4861 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 4921 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 4981 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 5041 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 5101 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt 5161 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 5221 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt 5281 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc 5341 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 5401 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 5461 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 5521 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 5581 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 5641 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 5701 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 5761 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 5821 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 5881 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 5941 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6001 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 6061 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 6121 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 6181 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 6241 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 6301 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 6361 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 6421 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 6481 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 6541 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 6601 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 6661 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 6721 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 6781 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 6841 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 6901 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 6961 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7021 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 7081 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 7141 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 7201 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 7261 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 7321 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 7381 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 7441 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 7501 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc 7561 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 7621 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 7681 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 7741 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 7801 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 7861 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 7921 ccagtgccaa gctg //