LOCUS       V046720                 9842 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046720
VERSION     V046720
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4863
                     /label="Ddr2(NM_022563)"
                     /note="Ddr2(NM_022563)"
                     /gene="Ddr2"
     CDS             4870..4896
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4900..4926
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4930..4956
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5007..5218
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5231..5499
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5532..5930
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     misc_feature    5937..6525
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6599..6832
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6904..7025
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7044..7179
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7217..7233)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7241..7257
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7265..7295)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7310..7331
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7619..8207)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8378..9238)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9239..9343)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9817..9833
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgatcccg attcccagaa tgcccctggt gctgctcctg
     2341 ctcttgctca tcctgggttc tgcaaaagct caggttaatc cagccatatg ccgctatcct
     2401 ctgggcatgt caggaggcca cattccagat gaggacatca cagcctcaag tcagtggtca
     2461 gaatccacgg ctgccaaata tgggaggctg gactctgaag aaggagatgg agcctggtgt
     2521 cctgagattc cagtgcaacc cgatgacctg aaggaatttc tgcagattga cttgcgaacc
     2581 ctacacttta tcactcttgt ggggacccag gggcgccatg cagggggtca tggcattgaa
     2641 tttgcaccca tgtacaagat caactacagt cgggatggca gtcgctggat ctcctggcgt
     2701 aaccggcatg ggaagcaggt gcttgatgga aacagtaacc cttatgatgt attcctgaag
     2761 gacttggagc cacccatcgt cgccagattt gttcgcctta tcccagtcac tgaccactcc
     2821 atgaacgtgt gcatgagggt tgagctttat ggttgtgtct ggctagatgg cttggtatcc
     2881 tacaatgctc cagctggaca gcagtttgta ctccctggag gctccatcat ttatctgaat
     2941 gattctgtct atgatggagc tgttgggtac agcatgactg aagggctagg ccagttgact
     3001 gatggagtat ccggcctgga tgattttacc cagacccatg aataccacgt gtggcctggc
     3061 tatgactacg tgggatggcg gaacgaaagt gctaccaacg gtttcattga gatcatgttt
     3121 gaatttgacc gaatcaggaa ttttactacc atgaaggtcc actgcaacaa catgtttgct
     3181 aaaggtgtga agatttttaa ggaggtccag tgctactttc gctcggaagc cagcgagtgg
     3241 gaacccactg ctgtctactt tcccctggtc ctggacgatg tgaaccccag tgcccggttt
     3301 gtcacggtgc ccctccacca ccgaatggcc agtgccatca agtgccaata ccattttgcc
     3361 gacacgtgga tgatgttcag cgagatcact ttccaatcag atgctgcaat gtataacaac
     3421 tctggagccc ttcccacctc tcctatggca cccaccacct atgatcccat gcttaaagtt
     3481 gatgatagca acactcggat cctgattggt tgcttggtgg ccatcatctt catcctgctg
     3541 gctatcatcg tcatcatcct gtggaggcag ttctggcaga agatgctaga aaaggcttca
     3601 cggaggatgc tggatgatga aatgacagtc agcctttccc tgcccagcga gtccagcatg
     3661 ttcaataaca accgctcctc atcaccaagt gaacaggagt ccaactctac ttatgatcga
     3721 atcttccccc ttcgccctga ctaccaggag ccatccagac tgatccgaaa gcttccagag
     3781 tttgctccag gagaggagga gtcagggtgc agtggtgttg tgaagccggc ccagcccaat
     3841 ggacctgagg gcgtgcccca ctatgcagaa gccgacatag tgaatctcca gggagtgaca
     3901 ggtggcaaca cctactgtgt gcctgctgta accatggatc tgctatcggg gaaagatgtg
     3961 gctgtggaag agttccccag gaaactgttg gccttcaagg agaagctggg agaaggccag
     4021 tttggggagg ttcatctctg tgaagtggag ggaatggaaa aattcaaaga caaagatttt
     4081 gcactagatg tcagtgccaa ccagcctgtc ctggtggccg tgaaaatgct ccgagcagat
     4141 gccaacaaga atgccaggaa tgattttctt aaggagatca agatcatgtc tcggctcaag
     4201 gacccaaaca tcatccgtct cttagctgtg tgcatcactg aggacccgct ctgcatgatc
     4261 acggaataca tggagaatgg agatcttaat cagtttcttt ctcgccacga gcctctgagt
     4321 tcctgttcta gtgatgccac agtcagttac gccaacctga agtttatggc aacccagatt
     4381 gcctctggta tgaagtacct ttcgtctctc aactttgtcc accgagatct ggccacacga
     4441 aactgtttag tgggcaagaa ttacaccatc aagatagctg attttggcat gagcagaaac
     4501 ctgtacagtg gtgattacta ccggatccag ggccgggcgg tgctccccat tcgctggatg
     4561 tcctgggaaa gcatcttgct gggcaaattc accacggcaa gtgatgtgtg ggcctttggg
     4621 gtgactctgt gggagacctt caccttttgc caggagcagc cctattccca gctgtcggat
     4681 gagcaggtta tcgagaacac tggagagttc ttccgagacc aagggaggca gatctatctc
     4741 cctcaaccag ccctttgccc cgactctgtg tataagctga tgctcagctg ctggagaaga
     4801 gaaaccaagc accggccatc cttccaggaa atacacctcc tgcttcttca gcaaggagcc
     4861 gagctcgagt acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat
     4921 tatgctagct atccttatga tgtcccagac tacgcatagg gatccgcggc cgcgaaggat
     4981 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     5041 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     5101 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     5161 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     5221 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     5281 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     5341 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     5401 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     5461 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     5521 gctaggccac catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     5581 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
     5641 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
     5701 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
     5761 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
     5821 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
     5881 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gtcgacaatc
     5941 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6001 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6061 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6121 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6181 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6241 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6301 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     6361 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6421 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6481 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga
     6541 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6601 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc
     6661 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6721 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6781 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     6841 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6901 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6961 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7021 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc
     7081 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     7141 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc
     7201 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7261 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7321 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7381 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7441 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7501 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7561 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7621 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7681 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7741 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7801 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7861 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7921 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7981 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8041 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8101 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8161 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8221 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8281 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8341 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8401 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8461 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8521 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8581 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8641 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8701 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8761 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8821 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8881 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8941 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9001 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9061 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9121 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9181 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9241 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9301 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9361 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     9421 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     9481 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     9541 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     9601 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     9661 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     9721 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     9781 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
     9841 tg
//
