LOCUS       V039489                10231 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039489
VERSION     V039489
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5061
                     /label="BCLAF1(NM_014739)"
                     /note="BCLAF1(NM_014739)"
                     /gene="BCLAF1"
     CDS             5068..5157
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        5201..5412
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5425..5693
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5720..6319
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6326..6914
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6988..7221
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7293..7414
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7433..7568
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7606..7622)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7630..7646
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7654..7684)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7699..7720
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8008..8596)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8767..9627)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9628..9732)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10206..10222
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgggtcgc tccaattcta gatcacattc ttcaaggtca
     2341 aagtctagat cacagtctag ttctcgatca agatcaagat ctcattctag aaagaagcga
     2401 tacagttcta ggtctcgttc cagaacatat tcaaggtctc gtagtagaga tcgtatgtat
     2461 tctagagatt atcgtcgcga ttacagaaat aatagaggaa tgagacgacc ttatgggtac
     2521 agaggaaggg gtagagggta ttatcaagga ggaggaggta gatatcatcg aggtggttat
     2581 agacctgtct ggaatagaag gcactctagg agtcctagac gaggtcgttc acgttccagg
     2641 agtccaaaaa gaagatccgt ttcttctcaa agatccagaa gcagatctcg ccggtcatat
     2701 agatcttcta ggtctccaag atcatcctct tctcgttctt catccccata tagcaaatct
     2761 cctgtttcta aaagacgagg gtctcaggaa aaacaaacca aaaaagctga aggggaaccc
     2821 caagaagaga gtccgttgaa aagtaaatca caggaggaac cgaaagatac atttgaacat
     2881 gacccatctg agtctatcga tgaatttaat aagtcatcag ccacatccgg tgatatttgg
     2941 cctggccttt cagcttatga taatagtcct agatcacccc atagtccttc acctattgct
     3001 acaccaccta gtcagagttc atcttgctct gatgctccca tgctcagtac agttcactct
     3061 gcaaaaaata ctccttctca gcattcacat tccattcagc atagtcctga aaggtctggg
     3121 tctggttctg ttggaaatgg atctagtcga tacagtcctt ctcagaatag tccaattcat
     3181 cacatccctt cacgaagaag tcctgcaaag acaatcgcac cacagaatgc tccaagagat
     3241 gagtctaggg gccgttcctc gttttatcct gatggtggag atcaggaaac tgcaaagact
     3301 gggaagttct taaaaaggtt cacagatgaa gagtctagag tattcctgct tgataggggt
     3361 aataccaggg ataaagaggc ttcaaaagag aaaggatcag agaaagggag ggcagaggga
     3421 gaatgggaag atcaggaagc tctagattac ttcagtgata aagagtctgg aaaacaaaag
     3481 tttaatgatt cagaagggga tgacacagag gagacagagg attatagaca gttcaggaag
     3541 tcagtcctcg cagatcaggg taaaagtttt gctactgcat ctcaccggaa tactgaggag
     3601 gaaggactca agtacaagtc caaagtttca ctgaaaggca atagagaaag tgatggattt
     3661 agagaagaaa aaaattataa acttaaagag actggatatg tagtggaaag gcctagcact
     3721 acaaaagata agcacaaaga agaagacaaa aattctgaaa gaataacagt aaagaaagaa
     3781 actcagtcac ctgagcaggt aaagtctgaa aagctcaaag acctctttga ttacagtccc
     3841 cctctacaca agaatctgga tgcacgagaa aagtctacct tcagagagga aagcccactt
     3901 aggatcaaaa tgatagcgag tgattctcac cgtcctgaag tcaaactcaa aatggcacct
     3961 gttcctcttg atgattctaa cagacctgct tccttgacta aagacaggct gcttgctagt
     4021 acacttgtcc attctgtcaa gaaggagcaa gaattccgat ccatctttga ccacattaag
     4081 ttgccacagg ccagcaaaag cacttcagag tcatttattc aacacattgt gtccttggtt
     4141 catcatgtta aagagcaata cttcaagtca gctgcaatga ccctaaacga gcggttcact
     4201 tcgtatcaga aagccactga agaacatagt actcggcaaa agagccctga aatacacagg
     4261 agaattgaca tctcaccaag taccctgagg aagcataccc gtttagcagg ggaagagaga
     4321 gtttttaaag aagaaaatca aaagggagat aaaaaattaa ggtgtgactc tgctgacctt
     4381 cggcatgaca ttgatcgccg tagaaaagaa agaagtaaag aacggggaga ttccaagggc
     4441 tccagggaat ccagtggatc aagaaagcag gaaaaaactc caaaagatta caaggaatac
     4501 aaatcttaca aagatgacag taaacataaa agagagcaag atcattctcg atcttcatcc
     4561 tcttcagcat caccttcttc tcccagttct cgagaagaaa aggagagtaa gaaggaaaga
     4621 gaagaagaat ttaaaactca ccatgaaatg aaagaatact caggctttgc aggagttagc
     4681 cgaccacgag gaaccttttt tcgaattaga ggcagaggaa gagccagagg agtttttgct
     4741 gggacaaata ctggtccaaa caactcaaat actacttttc aaaagagacc gaaggaagag
     4801 gaatgggatc cagaatatac cccaaagagc aagaagtact tcttgcatga cgacagagat
     4861 gatggtgtgg attattgggc caaaagagga agaggtcgtg gtacttttca acgtggcaga
     4921 gggcgcttta acttcaaaaa atcaggtagc agtcctaaat ggactcatga caaataccaa
     4981 ggggatggga ttgttgaaga tgaagaagag accatggaaa ataatgaaga aaagaaggac
     5041 agacgcaagg aagaaaagga aggatccgaa caaaaactaa tatcagagga agatttggaa
     5101 cagaaactaa tcagtgaaga agatttagag caaaagttaa tttccgagga ggacttatag
     5161 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     5221 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     5281 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     5341 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     5401 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     5461 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     5521 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     5581 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     5641 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     5701 tgaccggcgc ctacgctaga tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     5761 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     5821 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     5881 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     5941 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     6001 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     6061 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     6121 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     6181 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     6241 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     6301 ccgcaagccc ggtgcctgag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     6361 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6421 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6481 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6541 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6601 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6661 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6721 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6781 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6841 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6901 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta
     6961 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag
     7021 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7081 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7141 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7201 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7261 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     7321 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7381 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     7441 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7501 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7561 tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc
     7621 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7681 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7741 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7801 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7861 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7921 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7981 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8041 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8101 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8161 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8221 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8281 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8341 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8401 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     8461 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8521 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8581 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8641 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8701 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8761 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8821 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8881 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8941 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9001 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9061 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9121 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9181 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9241 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9301 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9361 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9421 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9481 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9541 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9601 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9661 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9721 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     9781 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     9841 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     9901 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     9961 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    10021 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
    10081 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    10141 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    10201 acgttgtaaa acgacggcca gtgccaagct g
//
