LOCUS V038651 9970 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038651 VERSION V038651 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4824 /label="BACH2(NM_021813)" /note="BACH2(NM_021813)" /gene="BACH2" CDS 4831..4896 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4940..5151 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 5164..5432 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 5459..6058 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6065..6653 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6727..6960 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7032..7153 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7172..7307 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7345..7361) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7369..7385 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7393..7423) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7438..7459 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7747..8335) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8506..9366) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9367..9471) /gene="bla" /label="AmpR promoter" primer_bind 9945..9961 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgtctgtg gatgagaagc ctgactcccc catgtatgtg 2341 tatgagtcca cagtccactg caccaacatc ctcctgggcc tcaatgacca gcggaaaaag 2401 gatattctct gtgacgtgac tttgatcgtg gagaggaagg agttccgggc ccaccgggct 2461 gtgctggccg catgcagtga atatttttgg caggcgctgg ttggacagac aaaaaatgat 2521 ttggtggtca gcttgcctga ggaggtcaca gccaggggct ttgggccgct gttacagttt 2581 gcctacactg ccaagctgtt actcagcaga gaaaacatcc gcgaggtcat ccgctgtgct 2641 gagttcctgc gcatgcacaa cctggaggac tcctgcttca gcttcctgca gacccagctc 2701 ctgaacagtg aggatggcct gtttgtgtgc cggaaggatg ctgcgtgcca gcgcccacac 2761 gaggactgcg agaactctgc aggagaggag gaggatgaag aggaggagac gatggattca 2821 gagacggcca agatggcttg ccccagggac cagatgcttc cagagcccat cagctttgag 2881 gccgccgcca tccccgtagc agagaaggaa gaagccctgc tgcccgagcc tgacgtgccc 2941 acagacacca aggagagctc agaaaaggac gcgttaacgc agtaccccag atacaagaaa 3001 taccagcttg catgtaccaa gaatgtctat aatgcatcat cacacagtac ctcaggtttt 3061 gcaagcacat tccgggaaga taactctagc aacagcctca agccggggct tgccaggggg 3121 cagattaaaa gtgagccgcc cagtgaagag aatgaggaag agagcatcac gctctgcctg 3181 tctggagatg agcctgacgc caaggacaga gcgggggatg tcgagatgga ccggaaacag 3241 cccagccctg cccctacccc cacggcccca gctggggccg cctgcctgga gagatccagg 3301 agcgtggcct cgccctcctg cttaaggtct ctgttcagca taacgaaaag tgtggagctg 3361 tctggcctgc ccagtacatc tcagcagcac tttgccagga gtccagcctg cccttttgac 3421 aaggggatca ctcagggtga ccttaaaact gactacaccc ctttcacagg gaattatgga 3481 cagccccacg tgggccagaa ggaggtgtcc aacttcacca tggggtcgcc cctcaggggg 3541 cctgggttgg aggctctctg taaacaggag ggagagctgg accggaggag cgtgatcttc 3601 tcctccagcg cttgtgacca agtgagcacc tcggtgcatt cttattctgg ggtgagcagt 3661 ttggacaaag acctctctga gccggtgcca aagggtctgt gggtgggagc cggccagtcc 3721 ctccccagct cgcaggccta ctcccacggt gggctgatgg ccgaccactt gccaggaagg 3781 atgcggccca acaccagctg cccggtacca atcaaagtct gccctcgctc accccccttg 3841 gagaccagga ccaggacttc cagctcctgc tcttcctatt cctacgcgga ggacgggagc 3901 gggggctcac cctgcagcct ccctctctgt gagttctcct cctcgccctg ttcccaggga 3961 gccagattcc ttgccacaga acatcaggaa ccaggcctga tgggagatgg aatgtacaac 4021 caagtgcggc cccaaattaa atgtgagcag tcttatggaa ccaactccag tgacgaatcc 4081 ggatcgttct cggaagcaga cagtgagtcg tgtcctgtgc aggacagggg ccaggaggta 4141 aaacttcctt ttcctgtaga tcaaatcaca gatcttccaa ggaacgattt ccagatgatg 4201 attaaaatgc acaagctaac ctcagaacag ttagagttta ttcatgatgt ccgacggcgc 4261 agcaagaacc gcatcgcggc ccagcgctgc cgcaaaagga aactggactg tattcagaat 4321 ttagaatgtg aaatccgcaa attggtgtgt gagaaagaga aactgttgtc agagaggaat 4381 caactgaaag catgcatggg ggaactgttg gacaacttct cctgcctttc ccaggaagtt 4441 tgccgagaca tccagagccc cgagcagatc caggccctgc atcggtattg ccctgtcctc 4501 agacccatgg acttgcccac ggcctccagt attaaccctg cgcccttggg tgctgagcag 4561 aacattgcgg cctcccaatg cgcagtgggg gaaaacgtgc cctgctgctt ggagccaggc 4621 gcggctcccc ccggaccccc ctgggcaccc agcaacacct ccgagaattg tacctctggg 4681 aggagactag aaggcactga cccgggaacc ttctcagaga gaggacctcc tcttgaaccc 4741 aggagccaaa cagtgaccgt ggacttctgc caggaaatga ctgataagtg tacaactgac 4801 gaacagccca ggaaagatta taccggatcc gactacaaag accatgacgg tgattataaa 4861 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat 4921 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 4981 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 5041 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 5101 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 5161 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 5221 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 5281 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 5341 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 5401 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagat 5461 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg 5521 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg 5581 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 5641 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 5701 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 5761 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 5821 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 5881 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 5941 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 6001 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt 6061 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 6121 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 6181 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 6241 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 6301 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 6361 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 6421 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct 6481 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 6541 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 6601 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac 6661 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 6721 ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg 6781 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 6841 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 6901 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 6961 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 7021 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 7081 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 7141 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc 7201 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 7261 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag 7321 agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 7381 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 7441 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 7501 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 7561 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 7621 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 7681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 7741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 7801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 7861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 7921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 7981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 8041 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 8101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 8161 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 8221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 8281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 8341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 8401 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 8461 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 8521 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 8581 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 8641 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 8701 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 8761 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 8821 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 8881 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 8941 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 9001 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 9061 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 9121 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 9181 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 9241 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 9301 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 9361 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 9421 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 9481 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 9541 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 9601 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 9661 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 9721 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 9781 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 9841 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 9901 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 9961 tgccaagctg //