LOCUS       V042958                11107 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042958
VERSION     V042958
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2306..2323
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     CDS             2324..5254
                     /label="Ap2a1(NM_007458)"
                     /note="Ap2a1(NM_007458)"
                     /gene="Ap2a1"
     promoter        5305..5516
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5529..5797
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5832..6539
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6549..6602
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6603..7202
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7203..7790
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7864..8097
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8169..8290
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8309..8444
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8482..8498)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8506..8522
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8530..8560)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8575..8596
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8884..9472)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9643..10503)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10504..10608)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11082..11098
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgcatca ccatcaccat cacatgccgg ctgtatccaa
     2341 aggcgatggc atgcgtgggc tcgccgtgtt catctccgac atccggaact gcaagagcaa
     2401 agaggctgag atcaagagga tcaacaagga actggccaac atccgttcca agttcaaagg
     2461 ggacaaggcc ttggatggct acagtaaaaa gaagtatgtg tgtaagctgc tcttcatatt
     2521 cctgcttggc catgacattg actttggaca tatggaggcc gtgaacctgc taagctctaa
     2581 caagtacacg gagaagcaga tagggtacct gttcatctca gtcctggtga actcgaactc
     2641 agaactgatc cggctcatca acaacgctat caagaatgac ctggccagtc gcaaccccac
     2701 cttcatgtgc ctggccttgc actgtatcgc taacgtgggc agccgtgaga tgggcgaggc
     2761 ttttgctgca gacattcccc gaatcctggt ggctggggac agcatggaca gtgtgaagca
     2821 gagtgccgcc ctatgcctac tgcgactcta caaggcctcg cccgacttgg tgcccatggg
     2881 cgagtggacg gcacgtgtag tgcacttgct caatgatcag cacatgggag tggtcacagc
     2941 tgctgtcagc ctcatcacct gtctctgcaa gaagaatccg gatgacttca agacctgtat
     3001 ctccctggct gtgtctcgct taagccggat cgtctcctca gcctccactg acctccagga
     3061 ctacacttac tacttcgttc ctgcaccctg gctctctgtg aagctactgc ggctgctcca
     3121 gtgttaccca ccaccagagg atgcagccgt gaaagggcgg ttagtggagt gtctggagac
     3181 tgtgctcaac aaggcccagg agcctcccaa gtccaagaag gtgcagcact ccaacgccaa
     3241 gaacgctatc ctctttgaga ccattagcct catcatccac tatgacagtg agcccaacct
     3301 cctggtccgc gcctgcaacc agctgggcca gttcctgcag caccgggaga ctaacctgcg
     3361 ctacctggcc ctggagagca tgtgcacgct ggccagctcc gagttctccc acgaggccgt
     3421 caagacccac attgatacag ttattaatgc cctcaagacg gagcgggacg tcagtgtgag
     3481 gcagcgggcg gctgatctcc tgtatgccat gtgtgaccgg agcaatgcca agcagattgt
     3541 gtcagagatg ctgcggtacc tggagactgc tgactatgcc atccgagagg agatcgtgct
     3601 gaaggtggcc atcctggctg agaagtatgc agtggactac agctggtacg tggacaccat
     3661 cctcaacctc atccgcatcg cgggcgacta tgtgagcgag gaggtgtggt accgcgtgct
     3721 gcagatcgtc accaaccgtg atgacgtcca gggttatgct gccaagacag tgtttgaggc
     3781 cctccaggcc ccagcctgtc atgagaacat ggtgaaggtc ggtggctaca tccttgggga
     3841 gtttgggaac ttgattgctg gggacccacg ctccagccca ccagtgcagt tctcgctgct
     3901 gcactccaag ttccacctgt gcagcgtggc cacccgcgct ctgttgctgt ccacctacat
     3961 caagttcatc aacctcttcc ctgagaccaa ggccaccatc caaggggttc tgcgtgccgg
     4021 ctcccagctg cgaaatgccg acgtggagct acagcagcgg gccgtggagt acctcaccct
     4081 cagctccgta gccagcaccg atgttctggc tacggtgcta gaagaaatgc ccccatttcc
     4141 cgagcgggag tcgtccatct tggccaagct gaagcgcaag aagggccctg gggcagccag
     4201 tgccctagat gacagccgca gggacaccag cagcaatgac atcaatgggg gtgtggagcc
     4261 cacccccagc actgtgtcga ccccctcacc ctccgcggac ctcttagggc tgcgggcagc
     4321 ccctccccct gctgcacccc cggctcccgt aggcgggaac ctcctggtgg atgtcttctc
     4381 tgacggcccc actgcacagc ccagcctggg gcccactcct gaggaggcct tcctcagcga
     4441 gctggagccc cctgcccctg agagccccat ggctttgttg gctgacccag ctccagctgc
     4501 tgacccaggt cctgaggaca taggccctcc cattccagaa gcagatgaac tgttgaataa
     4561 gttcgtgtgt aagaatagtg gggtcttgtt tgagaaccag ctgctgcaga ttggagtcaa
     4621 gtctgagttc cggcagaacc tgggccgcat gtatctcttc tatggcaaca agacttctgt
     4681 gcagttccag aacttcttgc ccaccgtggt ccatcctggg gacctccaga ctcagctggc
     4741 ggtgcagacc aagcgtgtgg cggcacaagt ggacggtggc gcacaggtgc agcaagtact
     4801 caacattgag tgtctgcgag acttcctgac gccgccactg ttgtcggtgc gcttccggta
     4861 cggtggcacc gcccagtccc tcactctgaa gctcccagtg accatcaaca aattcttcca
     4921 gcccacagag atggcggccc aagacttttt ccagcgctgg aagcagctga gcctccccct
     4981 gcaggaggca cagaaaatct tcaaagccaa ccacccgatg gatgctgaag ttactaaggc
     5041 caagcttctg gggtttggct ctgctcttct ggacaatgtg gatcccaacc ctgagaactt
     5101 tgtgggtgct ggaatcatcc agacgaaggc cctgcaggtg gggtgtctgc ttcggctgga
     5161 gcccaatgcc caggcccaaa tgtaccgtct aaccctgcgc accagcaaag agcctgtctc
     5221 ccgtcacttg tgtgagctgc tggcccagca gttctgagga tccgcggccg cgaaggatct
     5281 gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     5341 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
     5401 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     5461 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga
     5521 agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc
     5581 cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg
     5641 ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga
     5701 gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg
     5761 tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc
     5821 tagacgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     5881 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     5941 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     6001 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     6061 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     6121 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     6181 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     6241 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     6301 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     6361 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     6421 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     6481 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg
     6541 gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc
     6601 ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     6661 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     6721 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     6781 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     6841 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     6901 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     6961 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     7021 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     7081 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     7141 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     7201 gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7261 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7321 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7381 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7441 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7501 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7561 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     7621 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7681 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7741 tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc ctggtacctt
     7801 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     7861 gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt
     7921 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7981 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8041 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     8101 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     8161 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8221 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8281 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca
     8341 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     8401 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga
     8461 cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8521 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8581 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8641 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8701 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8761 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8821 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8881 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8941 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9001 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9061 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9121 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9181 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9241 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9301 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     9361 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9421 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9481 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9541 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9601 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9661 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9721 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9781 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9841 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9901 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9961 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10021 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10081 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10141 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10201 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10261 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10321 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10381 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10441 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10501 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10561 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10621 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    10681 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    10741 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    10801 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    10861 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    10921 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    10981 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    11041 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    11101 caagctg
//
