LOCUS       V035760                11770 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035760
VERSION     V035760
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5796
                     /label="Aff4(NM_033565)"
                     /note="Aff4(NM_033565)"
                     /gene="Aff4"
     CDS             5803..5868
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5912..6123
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6136..6404
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6433..6442
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6463..7125
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7195..7248
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7249..7848
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7849..8437
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8511..8744
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8816..8937
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8977..9112
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9145..9161)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9169..9185
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9193..9223)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9238..9259
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9547..10135)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10306..11166)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11167..11271)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11745..11761
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga accgtgaaga ccggaatgtg
     2341 ctgcgcatga aagaaaggga aaggcggaat caggaaattc agcagggtga agacgccttc
     2401 ccacctagtt ctcctctctt tgctgagcca tacaaagtca ctagcaaaga agataagtta
     2461 tcaagtcgga ttcagagcat gcttggaaac tacgatgaaa tgaaggatta cataggagac
     2521 agatcgatac caaagcttgt tgcaattccc aagcctgcag taccaacgac cacagacgag
     2581 aaagcaaacc caaatttttt tgagcagaga catggaggat ctcatcagag tagcaaatgg
     2641 actcctgtag gccccgcccc cagcacctct cagtctcaga aacgctcctc tgccctgcag
     2701 agtggacaca gcagccaacg gagtggtgca ggagggagtg gcgccagcag cagtggccag
     2761 aggcatgacc gtgactccta cagcagtagc aggaaaaaag gccagcacgg ctcagagcac
     2821 tccaaatctc gatcctcaag ccctggaaaa ccccaggctg tttcttcatt aagttctagt
     2881 cattccaggt ctcatgggaa tgatcaccat agcaaggaac atcagcgctc caaatcacct
     2941 cgggaccctg atgccaactg ggattctcct tcccgcggac ctttttcaag tgggcaacat
     3001 tcaagtcagt ctttcccacc ttctttgatg tcaaaatcta gttcaatgtt acagaaaccc
     3061 actgcctatg tacggcccat ggatggacag gagtctgtgg aaccaaagct gtcctctgag
     3121 cactacagca gccagtctca tggtaatagc atgactgagc tgaagcccag cagcaaagca
     3181 catctcacca agctgaaaat tccttcccga cctttggatg catcggtttc tggtgatgta
     3241 agctgtgtag atgaaatact taaagagatg acgcattcat ggcctccccc tctaacggct
     3301 attcatacac catgcaaaac agaaccttcc aaattccctt ttccaactaa ggagtcgcaa
     3361 cagtctaact ttggccctgg agaacaaaaa agatacagta ctgctaaaac atcaaatgga
     3421 caccagtcaa aatctatgtt aaaagatgac ttaaaactaa gcagcagtga agacagtgat
     3481 ggggaacagg attgtgataa gacaatgcca aggagtacac caggaagtaa ctctgaacct
     3541 tcacaccata acagtgaggg agcagacaac tccagggatg actcgagcag ccacagtgga
     3601 tctgagagca gctctgggtc tgactcggag agtgagagca gctccagtga cagtgaggct
     3661 aatgagccgt cgcagagcgc atctcctgag cctgaaccac caccaacaaa caagtggcaa
     3721 cttgataatt ggttgaataa agtgaaccca cataaagtat ccccagcttc ctctgtagac
     3781 agcaacatcc catcttctca agcctacaag aaagaaggcc gggagcaggg cactgccagt
     3841 aactacacag atccaggggg gacaaaagaa acgagctctg ccaccccagg acgagactct
     3901 aaaaccatcc aaaagggatc agaaagtggc cgtgggaggc agaagtctcc tgcacagagc
     3961 gacagtacca ctcagaggag aactgtcggc aaaaaacaac ccaaaaaacc tgagaagtca
     4021 gctgctgaag agcctcgtgg aggcctgaag atagaaagtg agacccctgt ggacatggct
     4081 gccagcatgc cctccagcag gcacaaagca gccaccaagg gctcgaggaa acccaacatc
     4141 aaaaaggaat caaaatcttc tcctcgacct acggcagaga agaagaagta caagtcagcc
     4201 agtaaaccgt cccagaagtc tcgggagatc atagagactg acacctcgtc ctcagactcg
     4261 gacggcagcg agagcctgcc tccctcctca cagactccta agtaccctga aagcaacagg
     4321 actcctgtca aaccctcctc agtggaggaa gaagatagct tttttcggca acgaatgttc
     4381 tctcctatgg aagagaagga gctcctttcc ccactcagtg agcctgatga caggtatcca
     4441 ctcattgtga agattgacct caatcttctg actcgaatac ccgggaagcc ttacaaagaa
     4501 acagagccac ccaaggggga aaagaaaaat gttccagaga aacactcgag agaggtgcag
     4561 aagcaagcct cagaaaaggc ttccaacaaa ggcaagagga aacacaagaa tgatgatgat
     4621 acccgagcca gtgagagcaa aaaacccaaa acagaggaca agaactcatc aggccacaag
     4681 ccatccagca gcagagagtc atctaagcag agtagtacaa aagaaaagga cttgctacct
     4741 tcacctgctg ggcccattct ttcaaaagat tcaaaaacag agcatggttc tcggaagagg
     4801 actgtcagtc agtcgtcgtc cttaaagtcc agtgggacca gcagcaaaga gaacagtggc
     4861 agcagcagca agagcagctc ctcctccaca gcgaagcaga agaagactga aggcaagggt
     4921 cccagcagct ccaaggaggc caaggagaag gctccaaata gctcctctaa ttgtcctccg
     4981 tctacaccaa cttctgagtc ttcgaagcct cggagaacaa agcttgcctt tgatgacaga
     5041 aattattcag cagaccatta tttacaagaa gcaaaaaaac tcaaacacaa tgcagatgct
     5101 ttgtctgaca ggtttgagaa agccgtgtac taccttgatg ctgtagtgtc tttcattgag
     5161 tgtgggaatg cattagagaa gaacgctcag gaatccaagt ccccattccc tatgtactca
     5221 gacacggtgg agctcattaa gtacactatg aaactaaaga attatttggc accagatgct
     5281 acagctgcag acaagagact cacagtactt tgcctgagat gccagtcatt attgtacctg
     5341 aggctgttca aactgaagaa ggagaacgct ctcaagtact ccaagacgct aactgagcac
     5401 ctgaagaatt cttacagtaa ttctcaagca ccttcacccg gcttggggag caaagctgtt
     5461 gggatgccct cccctgtttc tccaaaactg tcaccaggca attctggaag ttactcttct
     5521 gggggaagta gtgcttcagc aagtggttct tcagtgacca ttccacagaa aatccaccag
     5581 atggcagcaa gctatgtcca ggttacatct aacttcctct atgccacaga aatttgggac
     5641 caagctgaac agctttccaa agaacaaaaa gaattctttg ctgaactgga caaagtgatg
     5701 ggccctctca tctttaatgc aagcatcatg acagatctgg ctcgttacac ccggcaggga
     5761 ctgcattggc ttcggcagga tgccaagctg atatctggat ccgactacaa agaccatgac
     5821 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gcggccgcga
     5881 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     5941 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     6001 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     6061 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     6121 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     6181 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     6241 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     6301 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     6361 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     6421 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     6481 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     6541 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     6601 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     6661 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     6721 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     6781 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     6841 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     6901 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     6961 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     7021 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     7081 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     7141 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     7201 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     7261 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     7321 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     7381 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     7441 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     7501 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     7561 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     7621 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     7681 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     7741 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     7801 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     7861 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7921 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7981 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     8041 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8101 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8161 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8221 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     8281 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8341 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8401 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
     8461 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     8521 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga
     8581 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8641 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8701 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     8761 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     8821 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8881 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8941 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9001 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9061 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
     9121 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     9181 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9241 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9301 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9361 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9421 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9481 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9541 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9601 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9661 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9721 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9781 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9841 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9901 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9961 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    10021 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    10081 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    10141 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10201 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10261 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10321 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10381 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10441 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10501 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10561 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10621 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10681 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10741 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10801 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10861 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10921 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10981 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    11041 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    11101 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    11161 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11221 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11281 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    11341 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    11401 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    11461 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    11521 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    11581 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    11641 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    11701 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    11761 tgccaagctg
//
