LOCUS       V026328                11587 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026328
VERSION     V026328
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5613
                     /label="Adcy7(NM_001037723)"
                     /note="Adcy7(NM_001037723)"
                     /gene="Adcy7"
     CDS             5620..5685
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5729..5940
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5953..6221
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6250..6259
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6280..6942
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7012..7065
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7066..7665
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7666..8254
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8328..8561
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8633..8754
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8794..8929
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8962..8978)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8986..9002
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9010..9040)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9055..9076
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9364..9952)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10123..10983)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10984..11088)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11562..11578
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc cagccaaggg gcgctacttc
     2341 ctaaatgagg gtgatgaagg ccccgaccag gcagcgctct atgagaagta ccggctcacc
     2401 agcttgcacg ggccactgct gctcttgctc ctcctggtgg ccgcggccac ctgcattgcg
     2461 ctcatcagca tcgccttcag tcatgaggat ctccgcagac accaggttgt cctgggcact
     2521 gcgttcctca tgctgacgct gtttgtggct ctctatgtgc tggtgtatgt cgagtgcctg
     2581 gtgcagcggt ggctgcgggc cttggcgcta ctcacctggg cttgcctcat ggtactaggc
     2641 tccgtgctga tgtgggactc tttggagaat gaagcccatg cgtgggagca ggtgcctttc
     2701 ttcctgtttg tcgtctttgt ggtgtatgca ctactgcctc tcagcaggag ggcagccatc
     2761 gtggcaggcg tgacctccac ggtctcccat ctcctggtgt ttggagctgt gacaagagcc
     2821 ttccagacgt ccatgtctag cactcaactg gggctgcagc tcctggccaa tgccgttatc
     2881 ctcctgggtg ggaacttcac gggtgccttc cacaagcacc agctgcagga cgcgtccagg
     2941 gatctcttta tctacaccgt caaatgcatc cagatccgtc ggaagcttcg tgtggagaag
     3001 cgccagcagg agaacctgct tctgtcagtg ctcccagcac acatctccat gggtatgaag
     3061 ctggccatca ttgagcgcct caaagagggt ggtgaccgac actacatgcc cgacaacaac
     3121 tttcacagcc tctatgtcaa gcggcaccag aatgtcagca tcttgtatgc agacatcgtg
     3181 ggcttcacga ggctggccag cgactgctct cccaaggagc tggtggtggt gctcaacgag
     3241 ctgtttggga agtttgacca gattgctaag gccaatgagt gcatgcggat caagatcctg
     3301 ggtgactgtt actactgcgt gtcaggcctg cccgtgtcgc tgcccacaca tgcccgcaac
     3361 tgtgtgaaga tgggtctgga catctgcgag gccattaagc aggtgcgtga ggccacgggc
     3421 gtggacatca gcatgcgtgt gggcattcac tccgggaatg tgctatgtgg ggtcatcggg
     3481 ctccgtaagt ggcagtatga tgtgtggtcc catgatgtgt ccctggccaa caggatggag
     3541 gcagctggag tccctggccg ggtgcacatc acagaggcaa cattgaatca cctggacaag
     3601 gcatatgagg tggaggatgg gcatggggag cagcgagacc cctatctgaa agagatgaac
     3661 atccgaacct acctggtgat cgatccccgg agccagcagc cacccccacc cagccaccac
     3721 ctctccaagc ccaaggggga cgcaactctg aagatgcggg cttcagtgcg tgtaacccgc
     3781 tatctggagt cttggggggc agcaaggccc tttgcacacc tcaaccaccg ggagagtgtg
     3841 agcagcagtg agacccccat ctccaatgga cggaggcaga aggccattcc tctgcgtcga
     3901 caccgtgccc ctgataggag tgcatctccc aaggggcgct tggaagatga ctgtgatgac
     3961 gagatgctgt cagccattga gggtctcagc tccaccaggc cctgctgctc caagtctgat
     4021 gacttccaca cctttggtcc catcttcttg gagaagggct ttgagcgtga gtaccgcctg
     4081 gtgcccatcc cccgggctcg ctacgacttc gcctgtgcca gccttgtctt cgtctgcatc
     4141 ctgcttgtcc accttctagt gatgcccagg atggcaactc tgggtgtgtc ctttgggttg
     4201 gtggcctgcc tgctggggct ggttctgagt ttctgctttg ctactgagtt ctcgaggtgc
     4261 tttccatccc gaagtacact ccaggccatc tcggagagcg tggagacgca gcccctggtc
     4321 aggcttgtcc tggttgtgct gactgttggc agcctactga ctgtcgccat cattaacatg
     4381 ccactgacgc ttaacccagg cccagagcag cctggagaca acaagacaag cccactggct
     4441 gcacagaaca gagttgggac cccatgtgag ctcctcccgt actacacctg cagctgcatc
     4501 ctgggcttca ttgcatgctc tgttttcctg cggatgagcc tagagctgaa ggccatgctg
     4561 ctgacagtgg ccttggtggc ctacctgctg ctcttcaacc tctccccatg ctggcacgtc
     4621 tcaggcaaca gcactgagac caacgggaca caaaggacac ggctgctcct gtctgatgca
     4681 caaagcatgc ccagccacac ccttgctccg ggggctcggg agactgcccc ttctcccagt
     4741 tatttagaga gagacctgaa gatcatggtt aacttctacc tgatcctgtt ctatgccacc
     4801 ctcatcttgc tgtctagaca gattgactac tactgccgct tggactgtct gtggaagaag
     4861 aagttcaaaa aggagcacga ggagtttgaa acaatggaga atgtgaaccg cctcctcctg
     4921 gagaatgtgc tgccggcgca cgtggctgcc cacttcattg gggacaaggc agcagaggat
     4981 tggtaccatc aatcttatga ctgtgtctgt gtcatgtttg catccgttcc ggacttcaaa
     5041 gtgttctaca ctgagtgtga tgtcaacaaa gaaggactgg agtgccttcg actgctgaat
     5101 gagataattg ctgattttga cgagctcctg ctgaagccca agtttagtgg tgtggagaag
     5161 atcaagacca ttggcagcac ctacatggcg gcagcagggc tcagtgcccc ctcaggacat
     5221 gagaaccagg acctggagcg gaagcacgtg cacatcggag tcttggtaga atttagcatg
     5281 gccctgatga gcaagctgga tgggatcaac aggcactcct tcaactcctt ccgcctccga
     5341 gtcggcataa accacgggcc tgtgattgct ggagtgattg gagcacgcaa gcctcagtat
     5401 gacatctggg gaaacacagt caatgttgcc agccgcatgg agagcaccgg agagcttggg
     5461 aaaatccagg ttaccgaaga gacatgcact atcctccagg gactcggata ttcgtgtgaa
     5521 tgccgtgggc tgatcaacgt caaaggcaaa ggggaactgc ggacttactt tgtatgtaca
     5581 gacactgcca agtttcaagg gctggggcta aacggatccg actacaaaga ccatgacggt
     5641 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     5701 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     5761 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5821 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5881 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5941 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     6001 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     6061 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     6121 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     6181 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     6241 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     6301 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     6361 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     6421 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     6481 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     6541 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     6601 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     6661 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     6721 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     6781 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     6841 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     6901 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     6961 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     7021 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     7081 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     7141 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     7201 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     7261 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     7321 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     7381 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     7441 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     7501 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7561 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7621 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     7681 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7741 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7801 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7861 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7921 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7981 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     8041 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     8101 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8161 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8221 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     8281 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     8341 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     8401 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8461 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8521 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8581 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     8641 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8701 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8761 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8821 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8881 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     8941 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     9001 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     9061 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9121 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     9181 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9781 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     9841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10021 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    10081 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    10141 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10201 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10261 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10321 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10381 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10441 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10501 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10561 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10621 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10681 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10741 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10801 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10861 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10921 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10981 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    11041 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    11101 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    11161 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    11221 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    11281 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    11341 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    11401 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    11461 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    11521 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    11581 caagctg
//
