LOCUS       V039926                10246 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039926
VERSION     V039926
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..3372
                     /label="Ada(NM_007398)"
                     /note="Ada(NM_007398)"
                     /gene="Ada"
     CDS             4279..4344
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4388..4599
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4612..4880
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4909..4918
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4939..5601
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5671..5724
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5725..6324
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6325..6913
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6987..7220
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7292..7413
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7453..7588
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7621..7637)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7645..7661
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7669..7699)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7714..7735
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8023..8611)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8782..9642)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9643..9747)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10221..10237
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cccagacacc cgcattcaac
     2341 aaacccaaag tagagttaca cgtccacctg gatggagcca tcaagccaga aaccatctta
     2401 tactttggca agaagagagg catcgccctc ccggcagata cagtggagga gctgcgcaac
     2461 attatcggca tggacaagcc cctctcgctc ccaggcttcc tggccaagtt tgactactac
     2521 atgcctgtga ttgcgggctg cagagaggcc atcaagagga tcgcctacga gtttgtggag
     2581 atgaaggcaa aggagggcgt ggtctatgtg gaagtgcgct atagcccaca cctgctggcc
     2641 aattccaagg tggacccaat gccctggaac cagactgaag gggacgtcac ccctgatgac
     2701 gttgtggatc ttgtgaacca gggcctgcag gagggagagc aagcatttgg catcaaggtc
     2761 cggtccattc tgtgctgcat gcgccaccag cccagctggt cccttgaggt gttggagctg
     2821 tgtaagaagt acaatcagaa gaccgtggtg gctatggact tggctgggga tgagaccatt
     2881 gaaggaagta gcctcttccc aggccacgtg gaagcctatg agggcgcagt aaagaatggc
     2941 attcatcgga ccgtccacgc tggcgaggtg ggctctcctg aggttgtgcg tgaggctgtg
     3001 gacatcctca agacagagag ggtgggacat ggttatcaca ccatcgagga tgaagctctc
     3061 tacaacagac tactgaaaga aaacatgcac tttgaggtct gcccctggtc cagctacctc
     3121 acaggcgcct gggatcccaa aacgacgcat gcggttgttc gcttcaagaa tgataaggcc
     3181 aactactcac tcaacacaga cgaccccctc atcttcaagt ccaccctaga cactgactac
     3241 cagatgacca agaaagacat gggcttcact gaggaggagt tcaagcgact gaacatcaac
     3301 gcagcgaagt caagcttcct cccagaggaa gagaagaagg aacttctgga acggctctac
     3361 agagaatacc aaggcggcgg agggagtgga ggagggggaa gcggcggcgg agggagtcaa
     3421 ctactgttta gtaacgtcaa ctccatagag ttcacttcat gcaatgaaac tgtggtcatc
     3481 ccttgcatcg tccgtaatgt ggaggcgcaa agcaccgaag aaatgtttgt gaagtggaag
     3541 ttgaacaaat cgtatatttt catctatgat ggaaataaaa atagcactac tacagatcaa
     3601 aactttacca gtgcaaaaat ctcagtctca gacttaatca atggcattgc ctctttgaaa
     3661 atggataagc gcgatgccat ggtgggaaac tacacttgcg aagtgacaga gttatccaga
     3721 gaaggcaaaa cagttataga gctgaaaaac cgcacggttt cgtggttttc tccaaatgaa
     3781 aagatcctca ttgttatttt cccaattttg gctatactcc tgttctgggg aaagtttggt
     3841 attttaacac tcaaatataa atccagccat acgaataaga gaatcattct gctgctcgtt
     3901 gccgggctgg tgctcacagt catcgtggtt gttggagcca tccttctcat cccaggagaa
     3961 aagcccgtga agaatgcttc tggacttggc ctcattgtaa tctctacggg gatattaata
     4021 ctacttcagt acaatgtgtt tatgacagct tttggaatga cctctttcac cattgccata
     4081 ttgatcactc aagtgctggg ctacgtcctt gctttggtcg ggctgtgtct ctgcatcatg
     4141 gcatgtgagc cagtgcacgg cccccttttg atttcaggtt tggggatcat agctctagca
     4201 gaactacttg gattagttta tatgaagttt gtcgcttcca accagaggac tatccaacct
     4261 cctaggaata ggggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     4321 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     4381 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     4441 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     4501 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     4561 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     4621 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     4681 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     4741 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     4801 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     4861 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
     4921 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     4981 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
     5041 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     5101 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     5161 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     5221 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     5281 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     5341 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     5401 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     5461 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     5521 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     5581 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
     5641 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
     5701 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
     5761 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     5821 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     5881 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     5941 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     6001 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     6061 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     6121 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     6181 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     6241 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     6301 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
     6361 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6421 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6481 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6541 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6601 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6661 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     6721 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6781 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6841 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6901 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6961 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     7021 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7081 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7141 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7201 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7261 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7321 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7381 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7441 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7501 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7561 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     7621 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7681 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7741 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7801 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7861 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7921 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7981 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8041 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8101 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8161 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8221 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8281 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8341 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8401 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8461 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8521 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8581 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8641 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8701 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8761 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8821 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8881 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8941 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9001 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9061 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9121 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9181 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9241 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9301 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9361 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9421 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9481 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9541 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9601 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9661 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9721 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     9781 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     9841 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     9901 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     9961 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    10021 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    10081 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    10141 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    10201 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
