LOCUS       V038741                 9544 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038741
VERSION     V038741
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4398
                     /label="Acsl1(NM_001302163)"
                     /note="Acsl1(NM_001302163)"
                     /gene="Acsl1"
     CDS             4405..4470
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4514..4725
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4738..5006
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5033..5632
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5639..6227
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6301..6534
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6606..6727
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6746..6881
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(6919..6935)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6943..6959
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6967..6997)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7012..7033
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7321..7909)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8080..8940)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8941..9045)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9519..9535
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggaagtc catgaattgt tccggtattt tcgaatgcca
     2341 gagctgattg acattcggca gtacgtgcgc acccttccaa ccaacaccct catggggttt
     2401 ggggcttttg cagcgctcac caccttctgg tatgccacca ggcctaaggc cctgaagcca
     2461 ccatgtgacc tctccatgca gtcagtggaa atagcgggta ccactgatgg tattcgaaga
     2521 tcagcagtcc ttgaagatga caagctcttg gtgtactact acgacgatgt cagaaccatg
     2581 tacgatggct tccagagggg gattcaggtg tcaaataatg gtccttgttt aggttctcgg
     2641 aagccaaacc agccctatga gtggatttcc tacaaagagg tggcagaact ggctgagtgc
     2701 ataggctccg ggctgatcca gaaggggttc aagccttgct ccgagcagtt catcggcctc
     2761 ttctctcaaa acagacccga gtgggtgatc gtcgagcaag gatgcttctc ttactcaatg
     2821 gtggtcgtcc cgctctatga cacccttgga gctgacgcca tcacctacat agtgaacaaa
     2881 gctgaactct ctgtgatttt tgctgacaag ccagaaaaag ccaaactctt attagaaggt
     2941 gtagaaaaca agttaacacc atgccttaaa atcatagtca tcatggactc ctacggcagt
     3001 gatctggtgg aacgaggcaa gaagtgtggg gtggaaatca tcagcctcaa agctctggag
     3061 gaccttggaa gagtgaacag agtgaagccc aagcctccag aacccgaaga tcttgcgata
     3121 atttgtttca caagtggaac tacaggcaac cccaaaggag caatgatcac tcaccaaaac
     3181 attataaacg actgctcagg ttttataaaa gcaacagaga gtgcattcat cgcttccaca
     3241 gatgatgtgc tgatatcttt cttgcctctc gcccatatgt ttgagaccgt tgtagagtgt
     3301 gtaatgctgt gtcatggagc taagatagga tttttccaag gagatatcag gctgcttatg
     3361 gacgacctca aggtgcttca gcccaccatc ttccctgtgg ttcccaggct gctgaaccgg
     3421 atgttcgaca gaatttttgg acaagcaaac acttccttga agcgatggct gttggacttt
     3481 gcctccaaaa ggaaagaggc ggagcttcgc agtggcatcg tcagaaacaa cagcctgtgg
     3541 gataaactca tcttccacaa gatacagtcg agcctgggtg ggaaagtccg gctgatgatc
     3601 acaggagcag ccccggtgtc tgccacagtg ctgacgtttc tgaggacagc gctcggctgc
     3661 cagttctatg aaggctacgg acagaccgag tgcactgctg gttgctgcct gagcttgccc
     3721 ggagactgga cggcaggcca tgttggagcc cccatgcctt gcaattatgt aaagcttgtg
     3781 gatgtggaag aaatgaatta cctggcatcc aagggcgagg gtgaggtgtg tgtgaaaggg
     3841 gcaaatgtgt tcaaaggcta cttgaaagac ccagcaagaa cagctgaagc cctggataaa
     3901 gatggctggt tacacacggg ggacattgga aaatggctgc caaatggcac cttgaagatt
     3961 atcgacagga aaaagcacat atttaaacta gcccaaggag agtacatagc accagaaaag
     4021 attgaaaata tctacctgcg gagtgaagcc gtggcccagg tgtttgtcca cggagaaagc
     4081 ttgcaggcct ttctcatagc agttgtggta cccgacgttg agagcctacc gtcctgggca
     4141 cagaagagag gcttacaagg gtccttcgaa gaactgtgca ggaacaagga tatcaataaa
     4201 gctatcctgg acgacttgtt gaaacttggg aaggaagccg gtctgaagcc atttgaacag
     4261 gtcaaaggca ttgctgtgca cccggaatta ttttctattg acaacggcct tctgactcca
     4321 acactgaagg cgaagaggcc agagctacgg aactatttca ggtcgcagat agatgaactg
     4381 tacgccacca tcaagatcgg atccgactac aaagaccatg acggtgatta taaagatcat
     4441 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     4501 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     4561 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     4621 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     4681 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     4741 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     4801 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     4861 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     4921 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     4981 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga
     5041 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     5101 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     5161 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5221 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     5281 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     5341 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     5401 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     5461 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     5521 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     5581 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa
     5641 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5701 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5761 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5821 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5881 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5941 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6001 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6061 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6121 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6181 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa
     6241 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6301 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc
     6361 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6421 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6481 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     6541 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6601 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6661 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6721 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc
     6781 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     6841 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg
     6901 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6961 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7021 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7081 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7501 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7741 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     7801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7861 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7921 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7981 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8041 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8101 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8161 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8221 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8281 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8341 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8401 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8461 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8521 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8581 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8641 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8701 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8761 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8821 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8881 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8941 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9001 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9061 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     9121 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     9181 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     9241 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     9301 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     9361 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     9421 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     9481 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
     9541 gctg
//
