LOCUS V038741 9544 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038741 VERSION V038741 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4398 /label="Acsl1(NM_001302163)" /note="Acsl1(NM_001302163)" /gene="Acsl1" CDS 4405..4470 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4514..4725 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4738..5006 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 5033..5632 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5639..6227 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6301..6534 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6606..6727 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6746..6881 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6919..6935) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6943..6959 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6967..6997) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7012..7033 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7321..7909) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8080..8940) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8941..9045) /gene="bla" /label="AmpR promoter" primer_bind 9519..9535 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggaagtc catgaattgt tccggtattt tcgaatgcca 2341 gagctgattg acattcggca gtacgtgcgc acccttccaa ccaacaccct catggggttt 2401 ggggcttttg cagcgctcac caccttctgg tatgccacca ggcctaaggc cctgaagcca 2461 ccatgtgacc tctccatgca gtcagtggaa atagcgggta ccactgatgg tattcgaaga 2521 tcagcagtcc ttgaagatga caagctcttg gtgtactact acgacgatgt cagaaccatg 2581 tacgatggct tccagagggg gattcaggtg tcaaataatg gtccttgttt aggttctcgg 2641 aagccaaacc agccctatga gtggatttcc tacaaagagg tggcagaact ggctgagtgc 2701 ataggctccg ggctgatcca gaaggggttc aagccttgct ccgagcagtt catcggcctc 2761 ttctctcaaa acagacccga gtgggtgatc gtcgagcaag gatgcttctc ttactcaatg 2821 gtggtcgtcc cgctctatga cacccttgga gctgacgcca tcacctacat agtgaacaaa 2881 gctgaactct ctgtgatttt tgctgacaag ccagaaaaag ccaaactctt attagaaggt 2941 gtagaaaaca agttaacacc atgccttaaa atcatagtca tcatggactc ctacggcagt 3001 gatctggtgg aacgaggcaa gaagtgtggg gtggaaatca tcagcctcaa agctctggag 3061 gaccttggaa gagtgaacag agtgaagccc aagcctccag aacccgaaga tcttgcgata 3121 atttgtttca caagtggaac tacaggcaac cccaaaggag caatgatcac tcaccaaaac 3181 attataaacg actgctcagg ttttataaaa gcaacagaga gtgcattcat cgcttccaca 3241 gatgatgtgc tgatatcttt cttgcctctc gcccatatgt ttgagaccgt tgtagagtgt 3301 gtaatgctgt gtcatggagc taagatagga tttttccaag gagatatcag gctgcttatg 3361 gacgacctca aggtgcttca gcccaccatc ttccctgtgg ttcccaggct gctgaaccgg 3421 atgttcgaca gaatttttgg acaagcaaac acttccttga agcgatggct gttggacttt 3481 gcctccaaaa ggaaagaggc ggagcttcgc agtggcatcg tcagaaacaa cagcctgtgg 3541 gataaactca tcttccacaa gatacagtcg agcctgggtg ggaaagtccg gctgatgatc 3601 acaggagcag ccccggtgtc tgccacagtg ctgacgtttc tgaggacagc gctcggctgc 3661 cagttctatg aaggctacgg acagaccgag tgcactgctg gttgctgcct gagcttgccc 3721 ggagactgga cggcaggcca tgttggagcc cccatgcctt gcaattatgt aaagcttgtg 3781 gatgtggaag aaatgaatta cctggcatcc aagggcgagg gtgaggtgtg tgtgaaaggg 3841 gcaaatgtgt tcaaaggcta cttgaaagac ccagcaagaa cagctgaagc cctggataaa 3901 gatggctggt tacacacggg ggacattgga aaatggctgc caaatggcac cttgaagatt 3961 atcgacagga aaaagcacat atttaaacta gcccaaggag agtacatagc accagaaaag 4021 attgaaaata tctacctgcg gagtgaagcc gtggcccagg tgtttgtcca cggagaaagc 4081 ttgcaggcct ttctcatagc agttgtggta cccgacgttg agagcctacc gtcctgggca 4141 cagaagagag gcttacaagg gtccttcgaa gaactgtgca ggaacaagga tatcaataaa 4201 gctatcctgg acgacttgtt gaaacttggg aaggaagccg gtctgaagcc atttgaacag 4261 gtcaaaggca ttgctgtgca cccggaatta ttttctattg acaacggcct tctgactcca 4321 acactgaagg cgaagaggcc agagctacgg aactatttca ggtcgcagat agatgaactg 4381 tacgccacca tcaagatcgg atccgactac aaagaccatg acggtgatta taaagatcat 4441 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc 4501 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 4561 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 4621 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 4681 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 4741 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 4801 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 4861 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 4921 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 4981 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga 5041 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct 5101 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat 5161 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 5221 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 5281 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 5341 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 5401 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 5461 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 5521 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 5581 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa 5641 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 5701 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 5761 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 5821 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 5881 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 5941 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 6001 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 6061 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 6121 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 6181 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa 6241 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 6301 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 6361 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6421 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 6481 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 6541 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 6601 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 6661 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 6721 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 6781 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 6841 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 6901 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 6961 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 7021 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 7081 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 7141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 7201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 7261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 7321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 7381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 7441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 7501 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 7561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 7621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 7681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 7741 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 7801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7861 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 7921 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 7981 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 8041 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 8101 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 8161 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 8221 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 8281 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 8341 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 8401 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 8461 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 8521 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 8581 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 8641 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 8701 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 8761 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 8821 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 8881 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 8941 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 9001 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 9061 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 9121 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 9181 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 9241 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 9301 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 9361 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 9421 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 9481 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 9541 gctg //