LOCUS       V029408                10510 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029408
VERSION     V029408
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4536
                     /label="Acap1(NM_153788)"
                     /note="Acap1(NM_153788)"
                     /gene="Acap1"
     CDS             4543..4608
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4652..4863
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4876..5144
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5173..5182
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5203..5865
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5935..5988
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5989..6588
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6589..7177
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7251..7484
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7556..7677
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7717..7852
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7885..7901)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7909..7925
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7933..7963)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7978..7999
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8287..8875)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9046..9906)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9907..10011)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10485..10501
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga cggtcaagct agattttgaa
     2341 gaatgtctca aggactcacc ccgcttccga gcctcgattg aactggtgga aactgaagtg
     2401 tcagaattgg agacccgcct ggaaaagctg ctaaagctgg gttcctgcct cctggagagc
     2461 ggacagcatt accttgctgc cggtcgtgcc ttcgttgttg gcatctgtga cctggctcgc
     2521 ctgggtccac cagagcccat gatggcggag tgtctggaaa aattcaccgt gagtctgaac
     2581 cacaaactgg acagccatgc tgagcttctt gatgctactc aacatacact gcagcagcaa
     2641 atccagactc tggtcaagga aggtctccgg ggcttccgag aggctcgcag ggatttctgg
     2701 cgaggggctg agaacctgga ggctgctctt acccacaatg cagaggtccc caggcgccgt
     2761 gtccaagaag cagaggaggc tggaactgct ttgaggaccg ctcgagctgg gtaccggagt
     2821 cgggcattag attatgccct gcaggtcaat gtgattgagg ataagaggaa gtttgacatc
     2881 atggaattcg tactgcgttt ggttgaggcc caggccactt atttccagca gggccatgaa
     2941 gagctaaaca ggctggctca gtatcggaag gaattgggta cccagttgca caacctggtc
     3001 ttgaactcag ctcgacagaa gagagacatg gaacagaggc atgtgttact gaagcagaag
     3061 gagctaggtg gcgaggagcc agagccaagc ctaaaggagg ggcctagtgg cttggtaatg
     3121 gaaggacatc ttttcaaacg tgccagcaat gcatttaaga cctggagcag acgctggttc
     3181 accattcaga acaaccagct ggtttaccag aagaaatata aggaccctgt aaccgtggtg
     3241 gtagacgacc ttcgtctctg tacagtgaag ctctgtccag actcagaaag gcgtttctgt
     3301 ttcgaagtag tgtccaccag caagtcctgt ttcctccagg ctgactctga gcgtctcctg
     3361 cagctgtggg tcagtgctgt ccagagcagc attgcctcag ccttcagcca ggctcacctg
     3421 gagaacagcc ctcgggggcc aggccaggtc tcaggatacc atgccccggg ctctgctgca
     3481 actctggcct gtggcggggc agccagagga agggagtctg gaggagttgg tcaggtggcg
     3541 gcccaggtac aaagcgtgga tggaaatgcg caatgctgcg actgcaggga gccagcccca
     3601 gagtgggcca gcatcaacct tggcgtcact ctctgcattc agtgttctgg catccacagg
     3661 agcctcggag ttcatttctc caaagtccgc tctctgaccc ttgactcctg ggaaccagaa
     3721 ctggtgaagc ttatgtgcga gctggggaat gtcatcatca accagatcta tgaggcccgg
     3781 gtggaggcca tggcggtgaa gaagccaggg cccagctgct cccggcagga aaaggaagcc
     3841 tggattcatg ccaagtatgt ggagaaaaag ttcctgacca aacttcctga aatccgtgga
     3901 cgaagaggtg gcaggggacc cccaagagga catcctcctg tgcccccaaa gcctcccatc
     3961 aggccacact cgggcattgt cagatccaaa tcagagtgcc cgtctgatga catgggaagc
     4021 ctgcaccctg gggccctgct gtttcaagct gctggacatc ccccatctct tcctaccatg
     4081 gctgatgccc tggcccatgg agctgatgtc aactgggtca atgtgggcca ggggaatgcc
     4141 acacctctga tccgggccac agctgctaac tctcttctgg cctgcgagtt tctcctccaa
     4201 aatggggcga atgtgaacca agcagacagt gccggccggg gtcctctcca ccatgccacc
     4261 attcttggtc acacagggct cgcttgcctg ttcctgaaac ggggtgctga tctgggggct
     4321 cgagacacag aaggcaggga ccctctgact atcgcaatgg aaaccactaa tgctgacatc
     4381 gtaaccctgc tacgattggc aaagatgagg gaggctgaag cggcccaggg ccaggcagga
     4441 gacgagacgt atctcgacat attccgagac ttctccctca tggcgtcgga cgacccagag
     4501 aaactgagcc gtcgcagtca cgacctccat actctgggat ccgactacaa agaccatgac
     4561 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gcggccgcga
     4621 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4681 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     4741 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4801 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4861 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     4921 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     4981 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     5041 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     5101 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     5161 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     5221 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     5281 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     5341 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     5401 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     5461 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     5521 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     5581 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     5641 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     5701 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     5761 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     5821 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     5881 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     5941 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     6001 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     6061 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6121 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6181 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6241 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6301 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6361 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6421 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6481 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6541 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     6601 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6661 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6721 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6781 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6841 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6901 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6961 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7021 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7081 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7141 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
     7201 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7261 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga
     7321 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7381 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7441 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7501 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7561 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7621 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7681 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7741 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7801 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
     7861 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     7921 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7981 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8041 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8101 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8161 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8221 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8281 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8341 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8401 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8461 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8521 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8581 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8641 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8701 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     8761 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8821 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8881 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8941 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9001 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9061 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9121 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9181 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9241 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9301 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9361 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9421 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9481 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9541 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9601 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9661 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9721 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9781 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9841 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9901 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9961 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10021 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10081 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    10141 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    10201 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    10261 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    10321 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    10381 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    10441 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    10501 tgccaagctg
//
