LOCUS V029408 10510 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029408 VERSION V029408 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..4536 /label="Acap1(NM_153788)" /note="Acap1(NM_153788)" /gene="Acap1" CDS 4543..4608 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4652..4863 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4876..5144 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5173..5182 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5203..5865 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 5935..5988 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5989..6588 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6589..7177 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7251..7484 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7556..7677 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7717..7852 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7885..7901) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7909..7925 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7933..7963) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7978..7999 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8287..8875) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9046..9906) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9907..10011) /gene="bla" /label="AmpR promoter" primer_bind 10485..10501 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga cggtcaagct agattttgaa 2341 gaatgtctca aggactcacc ccgcttccga gcctcgattg aactggtgga aactgaagtg 2401 tcagaattgg agacccgcct ggaaaagctg ctaaagctgg gttcctgcct cctggagagc 2461 ggacagcatt accttgctgc cggtcgtgcc ttcgttgttg gcatctgtga cctggctcgc 2521 ctgggtccac cagagcccat gatggcggag tgtctggaaa aattcaccgt gagtctgaac 2581 cacaaactgg acagccatgc tgagcttctt gatgctactc aacatacact gcagcagcaa 2641 atccagactc tggtcaagga aggtctccgg ggcttccgag aggctcgcag ggatttctgg 2701 cgaggggctg agaacctgga ggctgctctt acccacaatg cagaggtccc caggcgccgt 2761 gtccaagaag cagaggaggc tggaactgct ttgaggaccg ctcgagctgg gtaccggagt 2821 cgggcattag attatgccct gcaggtcaat gtgattgagg ataagaggaa gtttgacatc 2881 atggaattcg tactgcgttt ggttgaggcc caggccactt atttccagca gggccatgaa 2941 gagctaaaca ggctggctca gtatcggaag gaattgggta cccagttgca caacctggtc 3001 ttgaactcag ctcgacagaa gagagacatg gaacagaggc atgtgttact gaagcagaag 3061 gagctaggtg gcgaggagcc agagccaagc ctaaaggagg ggcctagtgg cttggtaatg 3121 gaaggacatc ttttcaaacg tgccagcaat gcatttaaga cctggagcag acgctggttc 3181 accattcaga acaaccagct ggtttaccag aagaaatata aggaccctgt aaccgtggtg 3241 gtagacgacc ttcgtctctg tacagtgaag ctctgtccag actcagaaag gcgtttctgt 3301 ttcgaagtag tgtccaccag caagtcctgt ttcctccagg ctgactctga gcgtctcctg 3361 cagctgtggg tcagtgctgt ccagagcagc attgcctcag ccttcagcca ggctcacctg 3421 gagaacagcc ctcgggggcc aggccaggtc tcaggatacc atgccccggg ctctgctgca 3481 actctggcct gtggcggggc agccagagga agggagtctg gaggagttgg tcaggtggcg 3541 gcccaggtac aaagcgtgga tggaaatgcg caatgctgcg actgcaggga gccagcccca 3601 gagtgggcca gcatcaacct tggcgtcact ctctgcattc agtgttctgg catccacagg 3661 agcctcggag ttcatttctc caaagtccgc tctctgaccc ttgactcctg ggaaccagaa 3721 ctggtgaagc ttatgtgcga gctggggaat gtcatcatca accagatcta tgaggcccgg 3781 gtggaggcca tggcggtgaa gaagccaggg cccagctgct cccggcagga aaaggaagcc 3841 tggattcatg ccaagtatgt ggagaaaaag ttcctgacca aacttcctga aatccgtgga 3901 cgaagaggtg gcaggggacc cccaagagga catcctcctg tgcccccaaa gcctcccatc 3961 aggccacact cgggcattgt cagatccaaa tcagagtgcc cgtctgatga catgggaagc 4021 ctgcaccctg gggccctgct gtttcaagct gctggacatc ccccatctct tcctaccatg 4081 gctgatgccc tggcccatgg agctgatgtc aactgggtca atgtgggcca ggggaatgcc 4141 acacctctga tccgggccac agctgctaac tctcttctgg cctgcgagtt tctcctccaa 4201 aatggggcga atgtgaacca agcagacagt gccggccggg gtcctctcca ccatgccacc 4261 attcttggtc acacagggct cgcttgcctg ttcctgaaac ggggtgctga tctgggggct 4321 cgagacacag aaggcaggga ccctctgact atcgcaatgg aaaccactaa tgctgacatc 4381 gtaaccctgc tacgattggc aaagatgagg gaggctgaag cggcccaggg ccaggcagga 4441 gacgagacgt atctcgacat attccgagac ttctccctca tggcgtcgga cgacccagag 4501 aaactgagcc gtcgcagtca cgacctccat actctgggat ccgactacaa agaccatgac 4561 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gcggccgcga 4621 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 4681 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta 4741 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 4801 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 4861 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc 4921 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact 4981 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc 5041 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa 5101 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg 5161 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag 5221 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc 5281 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc 5341 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc 5401 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc 5461 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc 5521 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc 5581 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat 5641 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc 5701 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac 5761 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc 5821 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag 5881 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc 5941 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac 6001 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 6061 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 6121 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 6181 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 6241 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 6301 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 6361 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 6421 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 6481 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 6541 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg 6601 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 6661 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 6721 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 6781 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 6841 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 6901 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 6961 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc 7021 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 7081 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 7141 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac 7201 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 7261 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga 7321 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 7381 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 7441 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 7501 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 7561 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 7621 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 7681 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 7741 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 7801 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt 7861 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct 7921 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 7981 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 8041 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 8101 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 8161 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 8221 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 8281 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 8341 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 8401 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 8461 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 8521 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 8581 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 8641 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 8701 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 8761 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 8821 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 8881 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 8941 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 9001 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 9061 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 9121 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 9181 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 9241 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 9301 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 9361 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 9421 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 9481 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 9541 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 9601 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 9661 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 9721 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 9781 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 9841 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 9901 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 9961 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 10021 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 10081 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 10141 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 10201 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 10261 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 10321 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 10381 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 10441 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 10501 tgccaagctg //