LOCUS V042792 9964 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V042792 VERSION V042792 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4818 /label="ATG9A(NM_024085)" /note="ATG9A(NM_024085)" /gene="ATG9A" CDS 4825..4890 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4934..5145 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 5158..5426 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 5453..6052 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6059..6647 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6721..6954 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7026..7147 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7166..7301 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7339..7355) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7363..7379 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7387..7417) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7432..7453 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7741..8329) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8500..9360) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9361..9465) /gene="bla" /label="AmpR promoter" primer_bind 9939..9955 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggcgcag tttgacactg aataccagcg cctagaggcc 2341 tcctatagtg attcaccccc aggggaggag gacctgttgg tgcacgtcgc cgaggggagc 2401 aagtcacctt ggcaccatat tgaaaacctt gacctcttct tctctcgagt ttataatctg 2461 caccagaaga atggcttcac atgtatgctc atcggggaga tctttgagct catgcagttc 2521 ctctttgtgg ttgccttcac taccttcctg gtcagctgcg tggactatga catcctattt 2581 gccaacaaga tggtgaacca cagtcttcac cctactgaac ccgtcaaggt cactctgcca 2641 gacgcctttt tgcctgctca agtctgtagt gccaggattc aggaaaatgg ctcccttatc 2701 accatcctgg tcattgctgg tgtcttctgg atccaccggc ttatcaagtt catctataac 2761 atttgctgct actgggagat ccactccttc tacctgcacg ctctgcgcat ccctatgtct 2821 gcccttccgt attgcacgtg gcaagaagtg caggcccgga tcgtgcagac gcagaaggag 2881 caccagatct gcatccacaa acgtgagctg acagaactgg acatctacca ccgcatcctc 2941 cgtttccaga actacatggt ggcactggtt aacaaatccc tcctgcctct gcgcttccgc 3001 ctgcctggcc tcggggaagc tgtcttcttc acccgtggtc tcaagtacaa ctttgagctg 3061 atcctcttct ggggacctgg ctctctgttt ctcaatgaat ggagcctcaa ggccgagtac 3121 aaacgtgggg ggcaacggct agagctggcc cagcgcctca gcaaccgcat cctgtggatt 3181 ggcatcgcta acttcctgct gtgccccctc atcctcatat ggcaaatcct ctatgccttc 3241 ttcagctatg ctgaggtgct gaagcgggag ccgggggccc tgggagcacg ctgctggtca 3301 ctctatggcc gctgctacct ccgccacttc aacgagctgg agcacgagct gcagtcccgc 3361 ctcaaccgtg gctacaagcc cgcctccaag tacatgaatt gcttcttgtc acctcttttg 3421 acactgctgg ccaagaatgg agccttcttc gctggctcca tcctggctgt gcttattgcc 3481 ctcaccattt atgacgaaga tgtgttggct gtggaacatg tgctgaccac cgtcacactc 3541 ctgggggtca ccgtgaccgt gtgcaggtcc tttatcccgg accagcacat ggtgttctgc 3601 cctgagcagc tgctccgcgt gatcctcgct cacatccact acatgcctga ccactggcag 3661 ggtaatgccc accgctcgca gacccgggac gagtttgccc agctcttcca gtacaaggca 3721 gtgttcattt tggaagagtt gctgagcccc attgtcacac ccctcatcct catcttctgc 3781 ctgcgcccac gggccctgga gattatagac ttcttccgaa acttcaccgt ggaggtcgtt 3841 ggtgtgggag atacctgctc ctttgctcag atggatgttc gccagcatgg tcatccccag 3901 tggctatctg ctgggcagac agaggcctca gtgtaccagc aagctgagga tggaaagaca 3961 gagttgtcac tcatgcactt tgccatcacc aaccctggct ggcagccacc acgtgagagc 4021 acagccttcc taggcttcct caaggagcag gttcagcggg atggagcagc tgctagcctc 4081 gcccaagggg gtctgctccc tgaaaatgcc ctctttacgt ctatccagtc cttacaatct 4141 gagtctgagc ccctgagcct tatcgcaaat gtggtagctg gctcatcctg ccggggccct 4201 ccactgccca gagacctgca gggctccagg cacagggctg aagtcgcctc tgccctgcgc 4261 tccttctccc cgctgcaacc cgggcaggcg cccacaggcc gggctcacag caccatgaca 4321 ggctctgggg tggatgccag gacagccagc tccgggagca gcgtgtggga aggacagctg 4381 cagagcctgg tgctgtcaga atatgcatcc acagagatga gcctgcatgc cctctatatg 4441 caccagctcc acaagcagca ggcccaggct gaacctgagc ggcatgtatg gcaccgccgg 4501 gagagtgatg agagtggaga aagcgcccct gatgaagggg gagagggcgc ccgggccccc 4561 cagtctatcc ctcgctctgc tagctatccc tgtgcagcac cccggcctgg agctcctgag 4621 accactgccc tgcatggggg cttccagagg cgctacggtg gcatcacaga tcctggcaca 4681 gtgcccaggg ttccctctca tttctctcgg ctgcctcttg gagggtgggc agaagatggg 4741 cagtcggcat caaggcaccc tgagcccgtg cccgaagagg gctcggagga tgagctaccc 4801 cctcaggtgc acaaggtagg atccgactac aaagaccatg acggtgatta taaagatcat 4861 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc 4921 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 4981 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 5041 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 5101 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 5161 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 5221 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 5281 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 5341 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 5401 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga 5461 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct 5521 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat 5581 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 5641 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 5701 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 5761 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 5821 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 5881 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 5941 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 6001 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa 6061 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 6121 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 6181 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 6241 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 6301 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 6361 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 6421 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 6481 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 6541 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 6601 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa 6661 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 6721 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 6781 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6841 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 6901 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 6961 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 7021 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 7081 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 7141 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 7201 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 7261 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 7321 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 7381 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 7441 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 7501 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 7561 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 7621 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 7681 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 7741 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 7801 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 7861 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 7921 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 7981 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 8041 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 8101 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 8161 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 8221 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 8281 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 8341 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 8401 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 8461 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 8521 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 8581 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 8641 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 8701 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 8761 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 8821 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 8881 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 8941 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 9001 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 9061 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 9121 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 9181 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 9241 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 9301 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 9361 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 9421 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 9481 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 9541 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 9601 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 9661 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 9721 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 9781 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 9841 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 9901 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 9961 gctg //