LOCUS       V023945                10253 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023945
VERSION     V023945
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4254
                     /label="ARHGAP25(NM_001007231)"
                     /note="ARHGAP25(NM_001007231)"
                     /gene="ARHGAP25"
     CDS             4261..4287
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4291..4317
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4321..4347
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4412..4623
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4636..4904
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4933..4942
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4963..5625
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5695..5748
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5749..6348
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6349..6936
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7010..7243
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7315..7436
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7455..7590
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7628..7644)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7652..7668
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7676..7706)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7721..7742
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8030..8618)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8789..9649)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9650..9754)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10228..10244
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt ccctaaaatt gccaaggaac
     2341 tgggatttca acctgaaagt ggaggctgcg aaaatagctc ggtcaaggag tgtgatgact
     2401 ggcgagcaga tggctgcctt ccatccatcg tccaccccca acccgctgga gaggcccatc
     2461 aagatgggct ggctgaagaa gcagaggtcc atcgtgaaga actggcagca gaggtacttt
     2521 gtgctgaggg cgcagcagct ctactactac aaggatgaag aggacacgaa gccccagggc
     2581 tgcatgtatc taccaggatg tacaatcaag gagatcgcca caaacccaga agaagctggg
     2641 aagtttgtct ttgaaatcat tccagcctca tgggaccaga atcgcatggg acaggactcc
     2701 tatgtcctca tggccagctc tcaggcggag atggaggagt gggttaaatt cctcaggaga
     2761 gttgctggca caccctgtgg agcagtgttt ggccagcgct tggatgagac tgtggcctat
     2821 gaacagaaat tcggccccca tctggtgccc atcctggtgg agaaatgtgc agagttcatc
     2881 ctggagcacg gccggaatga agagggcatc ttccgtctgc ctgggcagga caacctggtg
     2941 aagcagctga gagacgcttt tgatgctggg gagcggccct cctttgacag agacacagat
     3001 gtgcacactg tggcttccct gttaaagctc tacctccgag acctcccaga gcccgtggtt
     3061 ccctggagcc agtacgaagg gttcctgctc tgtgggcagc tcacgaatgc ggatgaggca
     3121 aaggctcagc aggagttgat gaagcagctc tccatccttc ctcgtgacaa ctatagtctc
     3181 ctgagctaca tctgcaggtt cctacatgaa atacagctga actgtgctgt taacaagatg
     3241 agtgtggaca acctggctac tgtgattggt gtgaatctca tcaggtcgaa ggtcgaagac
     3301 cctgccgtga tcatgagagg gactcctcag atccaaagag tgatgactat gatgatcaga
     3361 gaccatgaag tcctcttccc caagtccaag gatatacccc tgtcaccccc tgcccagaaa
     3421 aatgacccca agaaagctcc agtggcccga agctctgtag gctgggatgc cactgaagac
     3481 ctccgaattt ctaggacaga cagcttcagt agcatgacaa gcgactctga tacaaccagc
     3541 cccaccggac agcagccgag cgatgcgttt ccggaggaca gcagcaaagt acccagggaa
     3601 aagccaggag actggaaaat gcaatctcgt aaaaggactc aaacactccc taaccggaaa
     3661 tgtttcttga catcagcttt tcagggtgcc aacagcagca aaatggagat ctttaaaaat
     3721 gaattctggt cgccttcctc agaggctaag gcaggggaag ggcacaggag aacgatgtct
     3781 caagacttgc gccaactttc tgactcccaa cggacttcca cctacgataa cgtcccttcc
     3841 ctgccagggt cccctgggga ggaagccagt gcactctctt cccaagcctg tgactccaag
     3901 ggagatactc ttgccagtcc aaactctgaa actgggcctg gaaaaaagaa ctctggagaa
     3961 gaggaaattg attctttgca gaggatggtc caagagctac gaaaggaaat agaaacacag
     4021 aagcaaatgt atgaggaaca gattaaaaac cttgagaagg aaaattatga cgtttgggct
     4081 aaagtggtga ggctcaatga agaactggag aaggaaaaga agaagtctgc agccctagag
     4141 atcagcctcc gcaacatgga gcgctcccgg gaggatgttg agaagaggaa caaggccttg
     4201 gaagaagaag tcaaggaatt tgtcaaatcc atgaaggaac ccaagaccga ggctctcgag
     4261 tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga ttatgctagc
     4321 tatccttatg atgtcccaga ctacgcatag ggatccctgc gatatccagc gcggccgcga
     4381 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4441 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     4501 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4561 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4621 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     4681 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     4741 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     4801 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     4861 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     4921 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     4981 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     5041 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     5101 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     5161 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     5221 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     5281 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     5341 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     5401 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     5461 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     5521 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     5581 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     5641 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     5701 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     5761 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     5821 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     5881 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     5941 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6001 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6061 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6121 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6181 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6241 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6301 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     6361 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6421 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6481 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6541 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6601 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6661 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6721 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     6781 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6841 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct
     6901 cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact
     6961 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7021 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac
     7081 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7141 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7201 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7261 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     7321 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7381 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7441 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7501 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7561 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt
     7621 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7681 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7741 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7801 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7861 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7921 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7981 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8041 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8101 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8161 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8221 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8281 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8341 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8401 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8461 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8521 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8581 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8641 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8701 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8761 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8821 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8881 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8941 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9001 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9061 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9121 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9181 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9241 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9301 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9361 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9421 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9481 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9541 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9601 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9661 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9721 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9781 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     9841 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     9901 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     9961 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
    10021 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    10081 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    10141 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    10201 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
