LOCUS       V041060                 9401 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041060
VERSION     V041060
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4233
                     /label="AR(NM_001348061)"
                     /note="AR(NM_001348061)"
                     /gene="AR"
     CDS             4240..4266
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4270..4296
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4300..4326
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4371..4582
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4595..4863
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4890..5489
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5496..6084
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6158..6391
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6463..6584
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6603..6738
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(6776..6792)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6800..6816
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6824..6854)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6869..6890
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7178..7766)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7937..8797)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8798..8902)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9376..9392
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggaagtg cagttagggc tgggaagggt ctaccctcgg
     2341 ccgccgtcca agacctaccg aggagctttc cagaatctgt tccagagcgt gcgcgaagtg
     2401 atccagaacc cgggccccag gcacccagag gccgcgagcg cagcacctcc cggcgccagt
     2461 ttgctgctgc tgcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag
     2521 cagcagcagc agcagcagca agagactagc cccaggcagc agcagcagca gcagggtgag
     2581 gatggttctc cccaagccca tcgtagaggc cccacaggct acctggtcct ggatgaggaa
     2641 cagcaacctt cacagccgca gtcggccctg gagtgccacc ccgagagagg ttgcgtccca
     2701 gagcctggag ccgccgtggc cgccagcaag gggctgccgc agcagctgcc agcacctccg
     2761 gacgaggatg actcagctgc cccatccacg ttgtccctgc tgggccccac tttccccggc
     2821 ttaagcagct gctccgctga ccttaaagac atcctgagcg aggccagcac catgcaactc
     2881 cttcagcaac agcagcagga agcagtatcc gaaggcagca gcagcgggag agcgagggag
     2941 gcctcggggg ctcccacttc ctccaaggac aattacttag ggggcacttc gaccatttct
     3001 gacaacgcca aggagttgtg taaggcagtg tcggtgtcca tgggcctggg tgtggaggcg
     3061 ttggagcatc tgagtccagg ggaacagctt cggggggatt gcatgtacgc cccacttttg
     3121 ggagttccac ccgctgtgcg tcccactcct tgtgccccat tggccgaatg caaaggttct
     3181 ctgctagacg acagcgcagg caagagcact gaagatactg ctgagtattc ccctttcaag
     3241 ggaggttaca ccaaagggct agaaggcgag agcctaggct gctctggcag cgctgcagca
     3301 gggagctccg ggacacttga actgccgtct accctgtctc tctacaagtc cggagcactg
     3361 gacgaggcag ctgcgtacca gagtcgcgac tactacaact ttccactggc tctggccgga
     3421 ccgccgcccc ctccgccgcc tccccatccc cacgctcgca tcaagctgga gaacccgctg
     3481 gactacggca gcgcctgggc ggctgcggcg gcgcagtgcc gctatgggga cctggcgagc
     3541 ctgcatggcg cgggtgcagc gggacccggt tctgggtcac cctcagccgc cgcttcctca
     3601 tcctggcaca ctctcttcac agccgaagaa ggccagttgt atggaccgtg tggtggtggt
     3661 gggggtggtg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc
     3721 gaggcgggag ctgtagcccc ctacggctac actcggcccc ctcaggggct ggcgggccag
     3781 gaaagcgact tcaccgcacc tgatgtgtgg taccctggcg gcatggtgag cagagtgccc
     3841 tatcccagtc ccacttgtgt caaaagcgaa atgggcccct ggatggatag ctactccgga
     3901 ccttacgggg acatgcgttt ggagactgcc agggaccatg ttttgcccat tgactattac
     3961 tttccacccc agaagacctg cctgatctgt ggagatgaag cttctgggtg tcactatgga
     4021 gctctcacat gtggaagctg caaggtcttc ttcaaaagag ccgctgaagg gaaacagaag
     4081 tacctgtgcg ccagcagaaa tgattgcact attgataaat tccgaaggaa aaattgtcca
     4141 tcttgtcgtc ttcggaaatg ttatgaagca gggatgactc tgggagaaaa attccgggtt
     4201 ggcaattgca agcatctcaa aatgaccaga cccggatcct acccatacga cgtacctgat
     4261 tatgcaactt atccttatga tgtacctgat tatgctagct atccttatga tgtcccagac
     4321 tacgcatagg cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg
     4381 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta
     4441 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     4501 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     4561 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
     4621 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     4681 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     4741 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     4801 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
     4861 atccaagctg tgaccggcgc ctacgctaga tgaccgagta caagcccacg gtgcgcctcg
     4921 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     4981 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     5041 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     5101 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     5161 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     5221 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     5281 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     5341 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     5401 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     5461 ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca acctctggat tacaaaattt
     5521 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5581 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5641 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5701 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5761 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5821 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5881 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     5941 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6001 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6061 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     6121 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     6181 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     6241 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6301 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6361 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     6421 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     6481 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6541 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     6601 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6661 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     6721 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
     6781 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6841 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6901 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6961 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7021 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7081 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7141 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7201 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7261 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7321 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7381 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7441 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7501 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7561 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7621 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7681 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7741 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7801 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7861 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7921 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7981 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8041 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8101 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8161 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8221 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8281 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8341 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8401 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8461 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8521 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8581 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8641 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8701 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8761 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8821 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8881 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     8941 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     9001 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     9061 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     9121 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     9181 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     9241 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     9301 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     9361 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
