LOCUS       V027533                10600 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027533
VERSION     V027533
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4626
                     /label="APP(NM_000484)"
                     /note="APP(NM_000484)"
                     /gene="APP"
     CDS             4633..4698
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4742..4953
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4966..5234
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5263..5272
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5293..5955
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6025..6078
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6079..6678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6679..7267
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7341..7574
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7646..7767
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7807..7942
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7975..7991)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7999..8015
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8023..8053)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8068..8089
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8377..8965)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9136..9996)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9997..10101)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10575..10591
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc tgcccggttt ggcactgctc
     2341 ctgctggccg cctggacggc tcgggcgctg gaggtaccca ctgatggtaa tgctggcctg
     2401 ctggctgaac cccagattgc catgttctgt ggcagactga acatgcacat gaatgtccag
     2461 aatgggaagt gggattcaga tccatcaggg accaaaacct gcattgatac caaggaaggc
     2521 atcctgcagt attgccaaga agtctaccct gaactgcaga tcaccaatgt ggtagaagcc
     2581 aaccaaccag tgaccatcca gaactggtgc aagcggggcc gcaagcagtg caagacccat
     2641 ccccactttg tgattcccta ccgctgctta gttggtgagt ttgtaagtga tgcccttctc
     2701 gttcctgaca agtgcaaatt cttacaccag gagaggatgg atgtttgcga aactcatctt
     2761 cactggcaca ccgtcgccaa agagacatgc agtgagaaga gtaccaactt gcatgactac
     2821 ggcatgttgc tgccctgcgg aattgacaag ttccgagggg tagagtttgt gtgttgccca
     2881 ctggctgaag aaagtgacaa tgtggattct gctgatgcgg aggaggatga ctcggatgtc
     2941 tggtggggcg gagcagacac agactatgca gatgggagtg aagacaaagt agtagaagta
     3001 gcagaggagg aagaagtggc tgaggtggaa gaagaagaag ccgatgatga cgaggacgat
     3061 gaggatggtg atgaggtaga ggaagaggct gaggaaccct acgaagaagc cacagagaga
     3121 accaccagca ttgccaccac caccaccacc accacagagt ctgtggaaga ggtggttcga
     3181 gaggtgtgct ctgaacaagc cgagacgggg ccgtgccgag caatgatctc ccgctggtac
     3241 tttgatgtga ctgaagggaa gtgtgcccca ttcttttacg gcggatgtgg cggcaaccgg
     3301 aacaactttg acacagaaga gtactgcatg gccgtgtgtg gcagcgccat gtcccaaagt
     3361 ttactcaaga ctacccagga acctcttgcc cgagatcctg ttaaacttcc tacaacagca
     3421 gccagtaccc ctgatgccgt tgacaagtat ctcgagacac ctggggatga gaatgaacat
     3481 gcccatttcc agaaagccaa agagaggctt gaggccaagc accgagagag aatgtcccag
     3541 gtcatgagag aatgggaaga ggcagaacgt caagcaaaga acttgcctaa agctgataag
     3601 aaggcagtta tccagcattt ccaggagaaa gtggaatctt tggaacagga agcagccaac
     3661 gagagacagc agctggtgga gacacacatg gccagagtgg aagccatgct caatgaccgc
     3721 cgccgcctgg ccctggagaa ctacatcacc gctctgcagg ctgttcctcc tcggcctcgt
     3781 cacgtgttca atatgctaaa gaagtatgtc cgcgcagaac agaaggacag acagcacacc
     3841 ctaaagcatt tcgagcatgt gcgcatggtg gatcccaaga aagccgctca gatccggtcc
     3901 caggttatga cacacctccg tgtgatttat gagcgcatga atcagtctct ctccctgctc
     3961 tacaacgtgc ctgcagtggc cgaggagatt caggatgaag ttgatgagct gcttcagaaa
     4021 gagcaaaact attcagatga cgtcttggcc aacatgatta gtgaaccaag gatcagttac
     4081 ggaaacgatg ctctcatgcc atctttgacc gaaacgaaaa ccaccgtgga gctccttccc
     4141 gtgaatggag agttcagcct ggacgatctc cagccgtggc attcttttgg ggctgactct
     4201 gtgccagcca acacagaaaa cgaagttgag cctgttgatg cccgccctgc tgccgaccga
     4261 ggactgacca ctcgaccagg ttctgggttg acaaatatca agacggagga gatctctgaa
     4321 gtgaagatgg atgcagaatt ccgacatgac tcaggatatg aagttcatca tcaaaaattg
     4381 gtgttctttg cagaagatgt gggttcaaac aaaggtgcaa tcattggact catggtgggc
     4441 ggtgttgtca tagcgacagt gatcgtcatc accttggtga tgctgaagaa gaaacagtac
     4501 acatccattc atcatggtgt ggtggaggtt gacgccgctg tcaccccaga ggagcgccac
     4561 ctgtccaaga tgcagcagaa cggctacgaa aatccaacct acaagttctt tgagcagatg
     4621 cagaacggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     4681 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     4741 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     4801 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     4861 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     4921 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     4981 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     5041 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     5101 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     5161 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     5221 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     5281 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     5341 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     5401 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     5461 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     5521 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     5581 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     5641 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     5701 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     5761 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     5821 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     5881 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     5941 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     6001 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     6061 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6121 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6181 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6241 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6301 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6361 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6421 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6481 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6541 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6601 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6661 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6721 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6781 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6841 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6901 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6961 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7021 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7081 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7141 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7201 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7261 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7321 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     7381 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7441 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7501 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7561 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     7621 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     7681 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7741 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     7801 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7861 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7921 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     7981 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8041 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8101 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8161 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8221 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8281 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8341 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8401 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8461 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8521 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8581 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8641 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8701 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8761 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8821 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8881 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8941 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9001 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9061 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9121 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9181 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9241 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9301 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9361 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9421 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9481 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9541 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9601 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9661 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9721 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9781 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9841 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9901 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9961 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10021 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10081 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10141 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    10201 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    10261 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    10321 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    10381 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    10441 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    10501 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    10561 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
