LOCUS       V039385                10698 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039385
VERSION     V039385
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4717
                     /label="AP5Z1(NM_014855)"
                     /note="AP5Z1(NM_014855)"
                     /gene="AP5Z1"
     CDS             4724..4813
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        4857..5068
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5081..5349
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5378..5387
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5408..6070
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6140..6193
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6194..6793
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6794..7381
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7455..7688
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7760..7881
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7900..8035
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8073..8089)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8097..8113
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8121..8151)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8166..8187
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8475..9063)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9234..10094)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10095..10199)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10673..10689
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgt tctcggcagg agcggagagt ttgctccacc aggccaggga
     2341 gatccaggac gaggagctga agaagttctg ttcccggatc tgtaaactgc tgcaggcgga
     2401 ggacttgggg ccggacaccc tcgactccct gcagaggctc ttcctcatca tctcagccac
     2461 gaagtacagc cggaggctgg agaagacatg cgtagacctg ctgcaggcca ccctcggcct
     2521 gcctgcatgc cccgagcagc tccaggtgct ttgcgccgcc atcctgcgag agatgtcccc
     2581 ctctgacagc ctcagcctgg cctgggacca cacgcagaac agccggcagc tgagcctggt
     2641 ggcctccgtt ctcttggccc agggtgacag aaacgaggag gtcagagccg tgggccaggg
     2701 cgtgctacga gcgctggaga gccggcagcc tgagggaccc agcctcagac acctcctccc
     2761 cgtcatggcc aaggtcgtgg tcctcagccc gggcaccctc caggaggacc aggccaccct
     2821 gctcagcaag cggctggtcg actggctgcg ctacgccagc ctccagcaag ggctcccaca
     2881 ctccggcggc ttcttctcca cgcccagggc ccggcagccg ggccccgtca ccgaggtgga
     2941 cggggcggta gccacagact tcttcacggt gctctccagc ggccaccgct tcacagacga
     3001 ccagtggctg aacgtgcagg ccttctctat gctgcgggcg tggctgctgc acagcggccc
     3061 cgagggcccg ggcaccctgg acacagatga caggtcagag caggagggct ccactctgtc
     3121 ggtgatctcc gccacctcct ctgccggccg cctgctgccg ccccgggagc ggcttcggga
     3181 ggtggccttc gagtactgcc agcgcctcat tgagcaaagt aaccgacgag ccctgaggaa
     3241 gggggactcc gacctgcaga aagcttgcct ggtggaggcc gtgctggtgc tggacgtgct
     3301 gtgccggcag gacccgtcct tcctgtaccg aagtctctcc tgcctgaagg ccctgcacgg
     3361 gcgggtgcgc ggggacccgg cctctgtgcg ggtgctgctg cccctcgccc acttcttcct
     3421 gagccacggg gaagcggctg cagtggactc ggaagccgtc taccagcacc tgttcaccag
     3481 gatcccggtg gagcagttcc acagccccat gctggccttt gaattcatcc agttctgcag
     3541 ggacaacctc cacctgttca gcgggcacct cagcaccctc agattgagct tccccaacct
     3601 ctttaagttc ctggcctgga acagcccacc cctcacctcc gagtttgtgg cgctcctccc
     3661 ggccctggtg gacgctggca cagccctgga gatgctgcac gcgctgctgg acctgccctg
     3721 cttgacggcg gtgctggacc tgcagctcag gtcagcaccg gctgcatccg agaggccact
     3781 ctgggacacc tctctcaggg cccccagctg cctggaggcc ttccgggacc cgcagttcca
     3841 gggtcttttc caatacctgc tgcgccccaa ggccagtggc gccactgaga ggttggcgcc
     3901 actccaccag ctgctgcagc ccatggccgg ctgtgcccgc gtggcccagt gtgcccaggc
     3961 cgtgcccacg ctgctgcagg cattcttctc agcagtgacc caggtggctg acgggtccct
     4021 gatcaaccag ctggcgctgc tgctcctggg caggagcgac tcgctctacc cggccccagg
     4081 gtacgctgcc ggtgtgcaca gtgtgctgag ttctcagttc ctggccctgt gtacgctgaa
     4141 accctccctg gtggtggagc tggcaagaga cctgctggag ttcctgggca gcgtgaatgg
     4201 tctctgcagc agggcgagcc tcgtcaccag cgtggtgtgg gccatcggcg agtacctgtc
     4261 ggtgacctac gatcggaggt gcaccgtgga gcagatcaac aagttcttcg aagccctgga
     4321 ggctctgcta ttcgaggtca cccagtgccg cccctctgct gccctgccca ggtgtccccc
     4381 ccaggtggtc accgtgctga tgaccacgct gacgaagctg gcctcccgga gccaagatct
     4441 gatccccagg gcctctttat tgctgtcaaa gatgaggacc ctggctcaca gtccagccac
     4501 cagctccacg cacagcgagg agggcgcgga agccatccgt acccgggcca cagagctgct
     4561 gaccctgctg aagatgccta gcgtggccca gtttgtgctc acacccagca cggaggtgtg
     4621 cagcccccgc tatcaccgcg atgccaacac ggccctgccc ctggccctgc gcacggtcag
     4681 ccggctggtg gagagggagg ccggcctcat gccaggggga tccgaacaaa aactaatatc
     4741 agaggaagat ttggaacaga aactaatcag tgaagaagat ttagagcaaa agttaatttc
     4801 cgaggaggac ttatagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     4861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     4921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     4981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     5041 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     5101 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     5161 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     5221 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     5281 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     5341 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag
     5401 cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt
     5461 cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa
     5521 gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg
     5581 cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa
     5641 caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt
     5701 gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac
     5761 cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt
     5821 ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag
     5881 cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc
     5941 catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga
     6001 gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat
     6061 cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc
     6121 cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga
     6181 gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     6241 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     6301 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     6361 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     6421 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     6481 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     6541 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     6601 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     6661 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6721 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     6781 gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6841 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6901 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6961 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7021 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7081 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7141 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     7201 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7261 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7321 tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg
     7381 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7441 agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc
     7501 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7561 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7621 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7681 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7741 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7801 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7861 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7921 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7981 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     8041 ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8101 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8161 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8221 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8281 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8341 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8401 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8461 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8521 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8581 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8641 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8701 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8761 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8821 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8881 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     8941 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9001 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9061 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9121 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9181 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9241 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9301 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9361 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9421 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9481 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9541 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9601 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9661 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9721 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9781 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9841 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9901 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9961 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10021 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10081 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10141 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10201 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
    10261 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
    10321 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
    10381 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
    10441 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
    10501 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
    10561 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
    10621 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
    10681 acggccagtg ccaagctg
//
