LOCUS       V038900                14080 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038900
VERSION     V038900
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..8106
                     /label="ANKRD31(NM_001372053)"
                     /note="ANKRD31(NM_001372053)"
                     /gene="ANKRD31"
     CDS             8113..8178
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        8222..8433
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8446..8714
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      8743..8752
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             8773..9435
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             9505..9558
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             9559..10158
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    10159..10747
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10821..11054
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    11126..11247
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      11287..11422
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11455..11471)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11479..11495
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11503..11533)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11548..11569
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11857..12445)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12616..13476)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13477..13581)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     14055..14071
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aggaaggcgt ccaggcccca
     2341 gactgggaca gtgatgaaac tgtgatagag ggttcagtga cggagagcga cctggaagaa
     2401 aaggagctgc cctggagaag gttgctgttt gatcaagacg catctcttaa atctgagttc
     2461 agtctgcatc ctgatacaag aggaatgtgc aaaggcatgc cttctcctga gattcaactt
     2521 ggatttaagc tcagagagga tctgcaagag caaatgaata aaaataagat gatgcctgtt
     2581 cttagcgagg atacaatatt acagtctcaa gatgaaacag aacgaaatca agctctgtta
     2641 cagactagaa aaaactgttc aatgttcatt gggtcgtttc gccagtctgg actttcattg
     2701 aaccaccaaa atattgaagg gccagaggct gaaagtcctg aagttttgcc acacatagaa
     2761 aaggaactaa gtgaaggcag agattctcct gaagttagcc ttctctcagg cactgctatt
     2821 acagtatctg atacagttgc tgtaaaggag acatcattag tagagccaga gaagatttta
     2881 gcagcaccaa atacattttt tgagcctaga aaggaagtca caatgaccat gacttctgaa
     2941 gaaactaagg atgaagaaag ctctcttgaa acctttgtgt ctgccttaga aagtttactt
     3001 acatcaccag aaagcaccca ggaggaaaga ttgtttgaat tagtaagtga ttttgatcgt
     3061 aaagaattga tgaacccatt gagtgactct cttagttcca tatcaatacc tttgaattcc
     3121 tggtcggcat gtcatagaga tttactagaa gatgcaaaag atgatgcatt gccagctgag
     3181 ttattggaag ccctgaacac attgtcagaa gccaaggtgg aaaccatctg tcacagaaaa
     3241 gagggaggta gcagtctcat tgccagaaat gaatgtttag aagtggagtt caatacgtct
     3301 cagaccaatg aagattgtac acaaatagca gagactctgc aagacccaaa tccatctgga
     3361 ttgcaaactt tggctcatca aaatatcact tcttgtgagc cactaagtaa taagagaaat
     3421 tcaaattcag tgacaaatag ctctgatcag gaaactgcgt gtgtgttgag gagatcatcc
     3481 agactagaaa aactgaaagt aagcagagat gcaaagtact cagatcacat gtataagatg
     3541 ccagaaaaga ttttaccaaa aatccttggc tgtgaggacc taacaaataa taactcttca
     3601 gctcagaatt tcagaatgca ggatccggct ttaatgattg atggtaaaga gaagaatatg
     3661 cattcagcaa ggttcaagaa tggaaaacag atcaggaaaa atgaacaatt ttcaggaaaa
     3721 aaagaaaaaa tgaaggtgaa caaaatatct cttcatagca ttaaccggag aaacattttt
     3781 ggagagaact tagtatataa ggctgctcta cacgatgatg ctgatcttgt tcatcattgt
     3841 ataaaaaaag gtggaaatgt taatcagcca agttatgctg gttggacagc acttcatgaa
     3901 gctagtgtag gaggatttta tcggacagca agtgaactat taaaaggtgg agcagatgta
     3961 aatatcaaag gattatacca gattactccc ctacatgatg cagtgatgaa tggacattat
     4021 aaggtggcag agttacttct tctgaatgga gctgatccat tatttagaaa tgatgatgga
     4081 aaatgtgctt tggatgaggc caaggattta tgtatgaagc gtctccttga gagatatatc
     4141 cctaaacatc aaaaatgcct tacatcagct caaaggagta gcattgaccc actagacata
     4201 gaggatgtgt accaacacaa gaaaccaaag ttcagttcta aaagtcacat ttggcatgtt
     4261 tataatgaaa attccaacag acagaagctg gaacatgtaa aagtcaataa aggaagcaaa
     4321 gcgagtttat ttataaataa agaagatgta tatgaatatt accaaaaaga tcccaaaaac
     4381 acaaaatttg gtaaatccaa gcataaacaa tcaactcttg accagatata ctctacagga
     4441 ctcagaaagg gcaatctcca taacgtcaaa gatcccaaca caaacgtacc aaaaggtata
     4501 ggaagaagaa aaacacaaca taaaaggacc caagtagatg atgtagactg taatccaaga
     4561 aagatactag ctgtctctcc ttccaggaga ataaacagat tggttaccta tcagcagcat
     4621 attccagaaa ctcataatga ccttccagaa gaattgtgtg aaccttccag cttaactctg
     4681 tcaagcctga gaaatggatt agatagcagt actgaggctt gttcagtttc caaagagaaa
     4741 cacatccaga acctggattt atcagatagt caagaagttc aatgcttgga attagaatct
     4801 gttgaccaaa ctgaagctgt ttctttccca gggcttttat tacataaaga aatcaagtta
     4861 ccagttgtta ctacagataa gcagcctcac actctccagg aacaacacca tgtactttat
     4921 aaatctcatg aaaacagtaa cttggtccca aaagatgaga gatttaacaa atgggaaaat
     4981 tctttcctat cctttgtaaa ggaaaattct gataatgatg atgatgatga ttgctctacc
     5041 tctgagaagg ctataacatc taaaaaggtg ttgtgttcta caggtggcaa aaaacactat
     5101 aattttaagg agaatttaac aaataaaaaa gaaatgggtt tccaacagtt tttactttct
     5161 gaagatcatc tttcacagga aaatgagtta aaagcagtca gcctaaccac acttccagaa
     5221 caggaagctg ttaatttttc ttattcagat aatgcagtta tttctgaaca tgttgcaaat
     5281 tatgaacaat gcatatttgg accttctttt gatcactcaa atggcaatcc tgagcaaaat
     5341 tccctggctt gtatgagaac acttttgaca catgaggctt caaagttgac taatcatgtg
     5401 gagctattca aaaagccaca ggattatatt cccagagcac caactttttt aatgaatcaa
     5461 acagatacac atattgtgga aaagatggca aagaattgtg acactgagag gaattacatt
     5521 gacagagacc aaaaaataat ttattcaaat gagcctttat ccatagttgc tcattctcaa
     5581 gtaatagaaa caactaaagt tgaaaaaagg agacaaaacc atctagaaag tgagactata
     5641 cacaatatag attctcattc cactgacaat atgagtaaag aattggccaa catctcaaaa
     5701 cttagtcaaa gagaaaagaa ggaaatttct cacaaaccag atgaggaatt aactaataac
     5761 atcagtggag atgaaataac tataagaaat tgtgaggaga taaaagaaaa aacggagtca
     5821 gaaattcaca tgcctactaa tagccaagaa cacaaaacag ttcagaattt cagaaaaaga
     5881 caaagtttct taaaaactac ctgcaaccta ggaatgaaag caggtaggat caacaagagg
     5941 aatgccagag gggaaagcca gcttcattta gctgtcagaa gaggaaatct gcccctagtg
     6001 aaagccctga tagaatctgg agcagatgtg aatctaaatg ataatgcagg ttggacacca
     6061 cttcatgagg catctaatga aggatctatt gatataattg tagagttatt aaaagctggt
     6121 gctaaggtta attgtgaaaa tatagatgga attctgccct tacatgatgc tgttgcaaac
     6181 aatcatttaa aggcagctga gattctacta caaaatggag caaaccctaa tcaaaaagat
     6241 caaaaacaga agagtgcttt ggatgaagca gatgatgaaa aaatgaaaga attactaaga
     6301 tcttatggtg ctattgagac tgttaataga gatgagagtg atgctatagt caatgaaaaa
     6361 attcctgctg tccggtctaa aagacataaa cagtgttttt gtgatgatgg caaaactatt
     6421 gactcatctt ccctttcaca ccaagaaaga tcaagagaaa gcctttctgt gcatcagacc
     6481 ctcagtgcca ttctacaaga tatagaagaa aaacaagaat atttattaga gtttgaaata
     6541 agaaaccctg aagatgcaga acaatacatt gaaaagatgt taaagataaa aaagattatg
     6601 gataatgtgc ttgccaagca gaaagcagaa agggatgatc tggctaaaaa atacagggta
     6661 tccatagagt catttaagca tggagccctg agagaacaat tggccaactt ggctgcaaga
     6721 cagaagagcc ttttagttgt ggcaaaaaaa cagaaaaaaa taagcctgaa aattcaaaac
     6781 tgtaggaatg ttacatcatt gccttgtctt agtttaagga agctcccacc cagatcagaa
     6841 atctctagtg aaaaagacag ccaagagctc actagtctgg aaaatttaga gcatccccaa
     6901 tcaggttcac tttctcctgt ttctggaagc atgcaggaaa cacaattgtc tctggaaact
     6961 tggaactaca gccaaaatac caacatttgt ctaaattcag aggcagtgag aaggggagaa
     7021 ttttctggaa atgatatgaa ttctaaacaa aatggcagtg attgtacctt ggatggtttt
     7081 ccaaaatcca gacattcaga tggcacagag aagaataaat taccatccca acctgttgct
     7141 tttattggtc aaacagaata ttcacagaaa gagaatgatc ttacagaagc tacagacaaa
     7201 gaccatgaat tctatgtttc ttccccagta atcggcaaat taaatatttc agaaactgca
     7261 agtgtccttg ccgaaaatgc tgcccatcca tcaaatatta tttgtgatca ggacctttcc
     7321 aattatgatc ccaaaagagg aaacagaaaa acaagttccc agcaatcacc tacaggggca
     7381 tcagaatctc tggcacatca agggattgct gtcttaggca gtgatacagt gcatcagatg
     7441 aaaccatatc ttaaaaaatc agtttctgtt gttccatgtg cagatgacag tcagatctcc
     7501 tcttcctctg gatctgggca acaagataca ataaaaaagg ctttaaacta cagtacagct
     7561 cctaaaaaga aatgtattca gataaaagat ttgatattac taggaagaat taacccagga
     7621 aataacattc tggaattcaa aacacaggag actacccaca aagccagtat tttattgaat
     7681 ggtaaactta aggtagaaag tggtcagatt tataaaaacc cagtcacctg gctcaaggat
     7741 cttctgggag gcaacagtta tgtgacctgg aattatgctt ggagtaaggt aacgtatctt
     7801 gggaaggagc ttttaaggta tgtttctgag gatgcaccca tacttccaga accaaactca
     7861 gtacctcagc aatatcaacc ttgtcttcca gaagttgctt gtttagatga cccagtacag
     7921 gaaccaaaca aatcaatgtt tgagaaaaca aaatttggac aaggaacttc cagagagtca
     7981 atgcaaagca gcccacgtta tctacaaata aatgaaatac tattaatcag tgatcaagaa
     8041 tttctcccat gccacataat ggatcagcat tggaagtttt gtgtggaatg tgaggaacta
     8101 acacccggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     8161 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     8221 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     8281 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     8341 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     8401 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     8461 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     8521 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     8581 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     8641 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     8701 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     8761 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     8821 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     8881 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     8941 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     9001 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     9061 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     9121 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     9181 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     9241 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     9301 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     9361 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     9421 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     9481 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     9541 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     9601 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     9661 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     9721 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     9781 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     9841 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     9901 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     9961 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
    10021 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
    10081 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
    10141 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
    10201 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
    10261 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
    10321 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
    10381 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
    10441 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
    10501 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
    10561 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
    10621 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
    10681 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
    10741 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
    10801 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
    10861 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    10921 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    10981 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    11041 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
    11101 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
    11161 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    11221 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
    11281 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    11341 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    11401 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
    11461 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    11521 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    11581 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    11641 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    11701 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    11761 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    11821 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    11881 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    11941 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    12001 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    12061 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    12121 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    12181 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    12241 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    12301 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    12361 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    12421 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    12481 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    12541 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    12601 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    12661 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    12721 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    12781 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    12841 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    12901 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    12961 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    13021 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    13081 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    13141 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    13201 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    13261 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    13321 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    13381 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    13441 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    13501 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    13561 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    13621 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    13681 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    13741 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    13801 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    13861 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    13921 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    13981 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    14041 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
