LOCUS       V044085                 9971 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044085
VERSION     V044085
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4065
                     /label="ALCAM(NM_001627)"
                     /note="ALCAM(NM_001627)"
                     /gene="ALCAM"
     promoter        4130..4341
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4354..4622
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4651..4660
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4681..5343
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5413..5466
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5467..6066
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6067..6654
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6728..6961
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7033..7154
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7173..7308
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7346..7362)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7370..7386
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7394..7424)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7439..7460
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7748..8336)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8507..9367)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9368..9472)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9946..9962
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aatccaaggg ggccagttcc
     2341 tgccgtctgc tcttctgcct cttgatctcc gccaccgtct tcaggccagg ccttggatgg
     2401 tatactgtaa attcagcata tggagatacc attatcatac cttgccgact tgacgtacct
     2461 cagaatctca tgtttggcaa atggaaatat gaaaagcccg atggctcccc agtatttatt
     2521 gccttcagat cctctacaaa gaaaagtgtg cagtacgacg atgtaccaga atacaaagac
     2581 agattgaacc tctcagaaaa ctacactttg tctatcagta atgcaaggat cagtgatgaa
     2641 aagagatttg tgtgcatgct agtaactgag gacaacgtgt ttgaggcacc tacaatagtc
     2701 aaggtgttca agcaaccatc taaacctgaa attgtaagca aagcactgtt tctcgaaaca
     2761 gagcagctaa aaaagttggg tgactgcatt tcagaagaca gttatccaga tggcaatatc
     2821 acatggtaca ggaatggaaa agtgctacat ccccttgaag gagcggtggt cataattttt
     2881 aaaaaggaaa tggacccagt gactcagctc tataccatga cttccaccct ggagtacaag
     2941 acaaccaagg ctgacataca aatgccattc acctgctcgg tgacatatta tggaccatct
     3001 ggccagaaaa caattcattc tgaacaggca gtatttgata tttactatcc tacagagcag
     3061 gtgacaatac aagtgctgcc accaaaaaat gccatcaaag aaggggataa catcactctt
     3121 aaatgcttag ggaatggcaa ccctccccca gaggaatttt tgttttactt accaggacag
     3181 cccgaaggaa taagaagctc aaatacttac acactgacgg atgtgaggcg caatgcaaca
     3241 ggagactaca agtgttccct gatagacaaa aaaagcatga ttgcttcaac agctatcaca
     3301 gttcactatt tggatttgtc cttaaaccca agtggagaag tgactagaca gattggtgat
     3361 gccctacccg tgtcatgcac aatatctgct agcaggaatg caactgtggt atggatgaaa
     3421 gataacatca ggcttcgatc tagcccgtca ttttctagtc ttcattatca ggatgctgga
     3481 aactatgtct gcgaaactgc tctgcaggag gttgaaggac taaagaaaag agagtcattg
     3541 actctcattg tagaaggcaa acctcaaata aaaatgacaa agaaaactga tcccagtgga
     3601 ctatctaaaa caataatctg ccatgtggaa ggttttccaa agccagccat tcaatggaca
     3661 attactggca gtggaagcgt cataaaccaa acagaggaat ctccttatat taatggcagg
     3721 tattatagta aaattatcat ttcccctgaa gagaatgtta cattaacttg cacagcagaa
     3781 aaccaactgg agagaacagt aaactccttg aatgtctctg ctataagtat tccagaacac
     3841 gatgaggcag acgagataag tgatgaaaac agagaaaagg tgaatgacca ggcaaaacta
     3901 attgtgggaa tcgttgttgg tctcctcctt gctgcccttg ttgctggtgt cgtctactgg
     3961 ctgtacatga agaagtcaaa gactgcatca aaacatgtaa acaaggacct cggtaatatg
     4021 gaagaaaaca aaaagttaga agaaaacaat cacaaaactg aagcctaagg atccctgcga
     4081 tatccagcgc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     4141 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     4201 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     4261 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     4321 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     4381 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     4441 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     4501 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     4561 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     4621 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     4681 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     4741 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     4801 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     4861 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     4921 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     4981 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     5041 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     5101 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     5161 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     5221 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     5281 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     5341 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     5401 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     5461 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     5521 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     5581 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5641 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     5701 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     5761 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     5821 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     5881 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     5941 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6001 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6061 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6121 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6181 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6241 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6301 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6361 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6421 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     6481 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6541 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6601 cgtctccgcc ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgcctggta
     6661 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6721 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact
     6781 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6841 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6901 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6961 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     7021 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7081 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7141 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg
     7201 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7261 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca
     7321 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7381 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7441 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7501 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7561 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7621 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7681 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7741 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7801 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7861 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7921 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7981 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8041 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8101 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8161 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8221 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8281 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8341 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8401 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8461 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8521 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8581 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8641 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8701 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8761 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8821 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8881 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8941 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9001 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9061 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9121 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9181 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9241 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9301 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9361 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9421 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9481 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     9541 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     9601 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     9661 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     9721 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     9781 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     9841 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     9901 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     9961 gtgccaagct g
//
