LOCUS       V039983                10129 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039983
VERSION     V039983
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4959
                     /label="AGBL5(NM_021831)"
                     /note="AGBL5(NM_021831)"
                     /gene="AGBL5"
     CDS             4966..5055
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        5099..5310
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5323..5591
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5618..6217
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6224..6812
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6886..7119
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7191..7312
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7331..7466
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7504..7520)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7528..7544
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7552..7582)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7597..7618
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7906..8494)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8665..9525)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9526..9630)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10104..10120
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggagctg cgctgtgggg gattgctgtt cagttctcgc
     2341 tttgattcag ggaatctagc ccacgtggag aaggtggaat ctttgtccag tgatggggaa
     2401 ggggtaggag gtggggcgtc agccctgacc agtggcattg cctcttcccc tgactatgaa
     2461 ttcaacgtgt ggacccgacc agactgtgct gaaacggaat ttgagaatgg gaacaggtca
     2521 tggttctact tcagcgtccg gggaggaatg ccaggaaaac tcatcaagat caacattatg
     2581 aacatgaaca agcagagcaa gctgtattcc cagggcatgg ccccctttgt gcgcacactg
     2641 cccacccggc cacgctggga acgcattcga gaccggccca cctttgagat gacagagacg
     2701 cagtttgtgt tatcctttgt tcatcgtttc gtggagggcc gtggggccac caccttcttc
     2761 gccttctgct accccttctc ctacagtgac tgccaggaac tgctaaacca gctagaccag
     2821 cgctttccgg agaaccaccc tacccatagc agccccctgg ataccatcta ttaccatcgg
     2881 gagctccttt gctattctct ggatggactt cgtgtagatc tgctgacgat cacttcctgc
     2941 catgggcttc gagaagatcg agagccccgt ctagagcagc tatttcctga taccagcacc
     3001 cctcgaccat tccgtttcgc aggcaagagg atattcttct taagcagtag agtacaccca
     3061 ggggagactc catctagctt tgtcttcaat ggctttctgg acttcatcct ccgacctgat
     3121 gatccccggg cccaaaccct ccgtcgcctc ttcgtcttta agctgattcc catgttgaac
     3181 cccgatggtg tggtccgggg acactaccgc acagactcac gtggagtgaa tctgaaccgt
     3241 cagtacctga agcctgatgc cgtcctgcac ccggccatct atggggccaa agctgtgctt
     3301 ctctaccacc atgtgcactc tcgtctgaac tcccagagtt cctctgagca ccagcccagt
     3361 tcctgtctcc ctcctgatgc tcctgtttct gacctggaga aagccaacaa tctccaaaat
     3421 gaagctcagt gtgggcactc agctgacagg cataacgctg aagcctggaa acaaacagag
     3481 ccagcagaac agaagctcaa cagtgtgtgg attatgccac aacagtctgc ggggcttgaa
     3541 gagtcagccc ctgataccat cccccccaaa gagagtggcg ttgcttacta tgtggacctg
     3601 catggacatg cttccaaaag gggctgcttc atgtacggaa acagctttag tgatgagagc
     3661 acccaggtgg aaaacatgct atatccaaag ctcatctcct tgaattcagc ccacttcgac
     3721 ttccagggct gcaatttctc agagaagaat atgtatgccc gagaccgtag agatggccag
     3781 tctaaagagg gaagcggccg tgttgcaatc tacaaagcct cagggataat ccacagctac
     3841 acacttgaat gcaactacaa cactggacgc tcagtaaaca gcatccctgc tgcctgccat
     3901 gacaatgggc gtgccagccc ccctcccccg ccggctttcc cctccagata cactgtggaa
     3961 ctatttgagc aggtgggacg agctatggcc attgcagccc tggacatggc ggaatgtaat
     4021 ccgtggcccc gaattgtact gtcagagcac agcagcctta ctaatctacg ggcctggatg
     4081 ctgaaacatg tacgcaacag ccgaggccta agcagcactc tgaatgtggg tgtcaacaag
     4141 aagaggggcc ttcgaactcc acccaaaagt cacaatgggt tgcctgtctc ctgctccgaa
     4201 aacaccttga gtcgggcacg aagttttagc accggcacaa gtgccggtgg tagcagcagc
     4261 agccaacaaa attctccaca gatgaagaat tcccccagct ttccttttca tggcagtcgg
     4321 cctgcagggc tgccaggcct gggctctagt acccaaaagg tcacccaccg ggtgctgggc
     4381 cccgtcagag agccccgaag ccaggacagg agacggcagc agcagcccct gaaccatcgt
     4441 cctgcaggca gcctcgctcc atccccagct cctactagtt ctggcccagc ctcctcacac
     4501 aagctgggct cctgtctact gcctgattca ttcaacatac cagggagcag ttgctcactc
     4561 ttgtcctctg gagacaaacc agaggctgtc atggtaatcg ggaaaggtct gctagggact
     4621 ggagctcgga tgccctgcat caagactcga ttgcaggcta ggcccaggtt gggccggggc
     4681 tcaccgccga ctcgcagagg gatgaaaggc tcttcaggcc ccacatcccc taccccccgg
     4741 accagggaga gcagtgagct ggagctggga tcctgctctg ctacaccagg gctgcctcag
     4801 gccaggcccc cacggccccg ctctgcccct gccttttctc ctatatcctg tagtctatct
     4861 gactccccat cctggaattg ttacagcagg ggtcccttgg gccaacctga ggtttgtttt
     4921 gtccctaaat ctcccccact gactgtttct ccccgggtcg gatccgaaca aaaactaata
     4981 tcagaggaag atttggaaca gaaactaatc agtgaagaag atttagagca aaagttaatt
     5041 tccgaggagg acttatagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg
     5101 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     5161 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     5221 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     5281 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     5341 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     5401 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     5461 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     5521 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     5581 cgttacagat ccaagctgtg accggcgcct acgctagatg accgagtaca agcccacggt
     5641 gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc
     5701 cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga
     5761 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     5821 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     5881 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     5941 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6001 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6061 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     6121 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6181 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtc gacaatcaac ctctggatta
     6241 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6301 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6361 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6421 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6481 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6541 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6601 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     6661 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6721 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     6781 gagtcggatc tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca
     6841 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6901 actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6961 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7021 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7081 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     7141 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     7201 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7261 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7321 ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7381 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7441 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc
     7501 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7561 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7621 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7681 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7741 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7801 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7861 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7921 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7981 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8041 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8101 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8161 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8221 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8281 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8341 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8401 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8461 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8521 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8581 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8641 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8701 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8761 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8821 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8881 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8941 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9001 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9061 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9121 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9181 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9241 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9301 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9361 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9421 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9481 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9541 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9601 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9661 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     9721 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     9781 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     9841 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     9901 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     9961 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    10021 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    10081 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
