LOCUS       V040240                15073 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040240
VERSION     V040240
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..9099
                     /label="ABCA1(NM_005502)"
                     /note="ABCA1(NM_005502)"
                     /gene="ABCA1"
     CDS             9106..9171
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        9215..9426
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             9439..9707
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      9736..9745
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             9766..10428
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             10498..10551
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             10552..11151
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    11152..11740
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11814..12047
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    12119..12240
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      12280..12415
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(12448..12464)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12472..12488
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12496..12526)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12541..12562
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12850..13438)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13609..14469)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14470..14574)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     15048..15064
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cttgttggcc tcagctgagg
     2341 ttgctgctgt ggaagaacct cactttcaga agaagacaaa catgtcagct gctgctggaa
     2401 gtggcctggc ctctatttat cttcctgatc ctgatctctg ttcggctgag ctacccaccc
     2461 tatgaacaac atgaatgcca ttttccaaat aaagccatgc cctctgcagg aacacttcct
     2521 tgggttcagg ggattatctg taatgccaac aacccctgtt tccgttaccc gactcctggg
     2581 gaggctcccg gagttgttgg aaactttaac aaatccattg tggctcgcct gttctcagat
     2641 gctcggaggc ttcttttata cagccagaaa gacaccagca tgaaggacat gcgcaaagtt
     2701 ctgagaacat tacagcagat caagaaatcc agctcaaact tgaagcttca agatttcctg
     2761 gtggacaatg aaaccttctc tgggttcctg tatcacaacc tctctctccc aaagtctact
     2821 gtggacaaga tgctgagggc tgatgtcatt ctccacaagg tatttttgca aggctaccag
     2881 ttacatttga caagtctgtg caatggatca aaatcagaag agatgattca acttggtgac
     2941 caagaagttt ctgagctttg tggcctacca agggagaaac tggctgcagc agagcgagta
     3001 cttcgttcca acatggacat cctgaagcca atcctgagaa cactaaactc tacatctccc
     3061 ttcccgagca aggagctggc tgaagccaca aaaacattgc tgcatagtct tgggactctg
     3121 gcccaggagc tgttcagcat gagaagctgg agtgacatgc gacaggaggt gatgtttctg
     3181 accaatgtga acagctccag ctcctccacc caaatctacc aggctgtgtc tcgtattgtc
     3241 tgcgggcatc ccgagggagg ggggctgaag atcaagtctc tcaactggta tgaggacaac
     3301 aactacaaag ccctctttgg aggcaatggc actgaggaag atgctgaaac cttctatgac
     3361 aactctacaa ctccttactg caatgatttg atgaagaatt tggagtctag tcctctttcc
     3421 cgcattatct ggaaagctct gaagccgctg ctcgttggga agatcctgta tacacctgac
     3481 actccagcca caaggcaggt catggctgag gtgaacaaga ccttccagga actggctgtg
     3541 ttccatgatc tggaaggcat gtgggaggaa ctcagcccca agatctggac cttcatggag
     3601 aacagccaag aaatggacct tgtccggatg ctgttggaca gcagggacaa tgaccacttt
     3661 tgggaacagc agttggatgg cttagattgg acagcccaag acatcgtggc gtttttggcc
     3721 aagcacccag aggatgtcca gtccagtaat ggttctgtgt acacctggag agaagctttc
     3781 aacgagacta accaggcaat ccggaccata tctcgcttca tggagtgtgt caacctgaac
     3841 aagctagaac ccatagcaac agaagtctgg ctcatcaaca agtccatgga gctgctggat
     3901 gagaggaagt tctgggctgg tattgtgttc actggaatta ctccaggcag cattgagctg
     3961 ccccatcatg tcaagtacaa gatccgaatg gacattgaca atgtggagag gacaaataaa
     4021 atcaaggatg ggtactggga ccctggtcct cgagctgacc cctttgagga catgcggtac
     4081 gtctgggggg gcttcgccta cttgcaggat gtggtggagc aggcaatcat cagggtgctg
     4141 acgggcaccg agaagaaaac tggtgtctat atgcaacaga tgccctatcc ctgttacgtt
     4201 gatgacatct ttctgcgggt gatgagccgg tcaatgcccc tcttcatgac gctggcctgg
     4261 atttactcag tggctgtgat catcaagggc atcgtgtatg agaaggaggc acggctgaaa
     4321 gagaccatgc ggatcatggg cctggacaac agcatcctct ggtttagctg gttcattagt
     4381 agcctcattc ctcttcttgt gagcgctggc ctgctagtgg tcatcctgaa gttaggaaac
     4441 ctgctgccct acagtgatcc cagcgtggtg tttgtcttcc tgtccgtgtt tgctgtggtg
     4501 acaatcctgc agtgcttcct gattagcaca ctcttctcca gagccaacct ggcagcagcc
     4561 tgtgggggca tcatctactt cacgctgtac ctgccctacg tcctgtgtgt ggcatggcag
     4621 gactacgtgg gcttcacact caagatcttc gctagcctgc tgtctcctgt ggcttttggg
     4681 tttggctgtg agtactttgc cctttttgag gagcagggca ttggagtgca gtgggacaac
     4741 ctgtttgaga gtcctgtgga ggaagatggc ttcaatctca ccacttcggt ctccatgatg
     4801 ctgtttgaca ccttcctcta tggggtgatg acctggtaca ttgaggctgt ctttccaggc
     4861 cagtacggaa ttcccaggcc ctggtatttt ccttgcacca agtcctactg gtttggcgag
     4921 gaaagtgatg agaagagcca ccctggttcc aaccagaaga gaatatcaga aatctgcatg
     4981 gaggaggaac ccacccactt gaagctgggc gtgtccattc agaacctggt aaaagtctac
     5041 cgagatggga tgaaggtggc tgtcgatggc ctggcactga atttttatga gggccagatc
     5101 acctccttcc tgggccacaa tggagcgggg aagacgacca ccatgtcaat cctgaccggg
     5161 ttgttccccc cgacctcggg caccgcctac atcctgggaa aagacattcg ctctgagatg
     5221 agcaccatcc ggcagaacct gggggtctgt ccccagcata acgtgctgtt tgacatgctg
     5281 actgtcgaag aacacatctg gttctatgcc cgcttgaaag ggctctctga gaagcacgtg
     5341 aaggcggaga tggagcagat ggccctggat gttggtttgc catcaagcaa gctgaaaagc
     5401 aaaacaagcc agctgtcagg tggaatgcag agaaagctat ctgtggcctt ggcctttgtc
     5461 gggggatcta aggttgtcat tctggatgaa cccacagctg gtgtggaccc ttactcccgc
     5521 aggggaatat gggagctgct gctgaaatac cgacaaggcc gcaccattat tctctctaca
     5581 caccacatgg atgaagcgga cgtcctgggg gacaggattg ccatcatctc ccatgggaag
     5641 ctgtgctgtg tgggctcctc cctgtttctg aagaaccagc tgggaacagg ctactacctg
     5701 accttggtca agaaagatgt ggaatcctcc ctcagttcct gcagaaacag tagtagcact
     5761 gtgtcatacc tgaaaaagga ggacagtgtt tctcagagca gttctgatgc tggcctgggc
     5821 agcgaccatg agagtgacac gctgaccatc gatgtctctg ctatctccaa cctcatcagg
     5881 aagcatgtgt ctgaagcccg gctggtggaa gacatagggc atgagctgac ctatgtgctg
     5941 ccatatgaag ctgctaagga gggagccttt gtggaactct ttcatgagat tgatgaccgg
     6001 ctctcagacc tgggcatttc tagttatggc atctcagaga cgaccctgga agaaatattc
     6061 ctcaaggtgg ccgaagagag tggggtggat gctgagacct cagatggtac cttgccagca
     6121 agacgaaaca ggcgggcctt cggggacaag cagagctgtc ttcgcccgtt cactgaagat
     6181 gatgctgctg atccaaatga ttctgacata gacccagaat ccagagagac agacttgctc
     6241 agtgggatgg atggcaaagg gtcctaccag gtgaaaggct ggaaacttac acagcaacag
     6301 tttgtggccc ttttgtggaa gagactgcta attgccagac ggagtcggaa aggatttttt
     6361 gctcagattg tcttgccagc tgtgtttgtc tgcattgccc ttgtgttcag cctgatcgtg
     6421 ccaccctttg gcaagtaccc cagcctggaa cttcagccct ggatgtacaa cgaacagtac
     6481 acatttgtca gcaatgatgc tcctgaggac acgggaaccc tggaactctt aaacgccctc
     6541 accaaagacc ctggcttcgg gacccgctgt atggaaggaa acccaatccc agacacgccc
     6601 tgccaggcag gggaggaaga gtggaccact gccccagttc cccagaccat catggacctc
     6661 ttccagaatg ggaactggac aatgcagaac ccttcacctg catgccagtg tagcagcgac
     6721 aaaatcaaga agatgctgcc tgtgtgtccc ccaggggcag gggggctgcc tcctccacaa
     6781 agaaaacaaa acactgcaga tatccttcag gacctgacag gaagaaacat ttcggattat
     6841 ctggtgaaga cgtatgtgca gatcatagcc aaaagcttaa agaacaagat ctgggtgaat
     6901 gagtttaggt atggcggctt ttccctgggt gtcagtaata ctcaagcact tcctccgagt
     6961 caagaagtta atgatgccat caaacaaatg aagaaacacc taaagctggc caaggacagt
     7021 tctgcagatc gatttctcaa cagcttggga agatttatga caggactgga caccaaaaat
     7081 aatgtcaagg tgtggttcaa taacaagggc tggcatgcaa tcagctcttt cctgaatgtc
     7141 atcaacaatg ccattctccg ggccaacctg caaaagggag agaaccctag ccattatgga
     7201 attactgctt tcaatcatcc cctgaatctc accaagcagc agctctcaga ggtggctctg
     7261 atgaccacat cagtggatgt ccttgtgtcc atctgtgtca tctttgcaat gtccttcgtc
     7321 ccagccagct ttgtcgtatt cctgatccag gagcgggtca gcaaagcaaa acacctgcag
     7381 ttcatcagtg gagtgaagcc tgtcatctac tggctctcta attttgtctg ggatatgtgc
     7441 aattacgttg tccctgccac actggtcatt atcatcttca tctgcttcca gcagaagtcc
     7501 tatgtgtcct ccaccaatct gcctgtgcta gcccttctac ttttgctgta tgggtggtca
     7561 atcacacctc tcatgtaccc agcctccttt gtgttcaaga tccccagcac agcctatgtg
     7621 gtgctcacca gcgtgaacct cttcattggc attaatggca gcgtggccac ctttgtgctg
     7681 gagctgttca ccgacaataa gctgaataat atcaatgata tcctgaagtc cgtgttcttg
     7741 atcttcccac atttttgcct gggacgaggg ctcatcgaca tggtgaaaaa ccaggcaatg
     7801 gctgatgccc tggaaaggtt tggggagaat cgctttgtgt caccattatc ttgggacttg
     7861 gtgggacgaa acctcttcgc catggccgtg gaaggggtgg tgttcttcct cattactgtt
     7921 ctgatccagt acagattctt catcaggccc agacctgtaa atgcaaagct atctcctctg
     7981 aatgatgaag atgaagatgt gaggcgggaa agacagagaa ttcttgatgg tggaggccag
     8041 aatgacatct tagaaatcaa ggagttgacg aagatatata gaaggaagcg gaagcctgct
     8101 gttgacagga tttgcgtggg cattcctcct ggtgagtgct ttgggctcct gggagttaat
     8161 ggggctggaa aatcatcaac tttcaagatg ttaacaggag ataccactgt taccagagga
     8221 gatgctttcc ttaacaaaaa tagtatctta tcaaacatcc atgaagtaca tcagaacatg
     8281 ggctactgcc ctcagtttga tgccatcaca gagctgttga ctgggagaga acacgtggag
     8341 ttctttgccc ttttgagagg agtcccagag aaagaagttg gcaaggttgg tgagtgggcg
     8401 attcggaaac tgggcctcgt gaagtatgga gaaaaatatg ctggtaacta tagtggaggc
     8461 aacaaacgca agctctctac agccatggct ttgatcggcg ggcctcctgt ggtgtttctg
     8521 gatgaaccca ccacaggcat ggatcccaaa gcccggcggt tcttgtggaa ttgtgcccta
     8581 agtgttgtca aggaggggag atcagtagtg cttacatctc atagtatgga agaatgtgaa
     8641 gctctttgca ctaggatggc aatcatggtc aatggaaggt tcaggtgcct tggcagtgtc
     8701 cagcatctaa aaaataggtt tggagatggt tatacaatag ttgtacgaat agcagggtcc
     8761 aacccggacc tgaagcctgt ccaggatttc tttggacttg catttcctgg aagtgttcta
     8821 aaagagaaac accggaacat gctacaatac cagcttccat cttcattatc ttctctggcc
     8881 aggatattca gcatcctctc ccagagcaaa aagcgactcc acatagaaga ctactctgtt
     8941 tctcagacaa cacttgacca agtatttgtg aactttgcca aggaccaaag tgatgatgac
     9001 cacttaaaag acctctcatt acacaaaaac cagacagtag tggacgttgc agttctcaca
     9061 tcttttctac aggatgagaa agtgaaagaa agctatgtag gatccgacta caaagaccat
     9121 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     9181 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     9241 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     9301 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     9361 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     9421 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     9481 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     9541 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     9601 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     9661 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     9721 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc
     9781 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     9841 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     9901 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     9961 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
    10021 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
    10081 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
    10141 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
    10201 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
    10261 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
    10321 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
    10381 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
    10441 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag
    10501 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag
    10561 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
    10621 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
    10681 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
    10741 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
    10801 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
    10861 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
    10921 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
    10981 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
    11041 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
    11101 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct
    11161 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
    11221 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
    11281 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
    11341 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
    11401 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
    11461 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
    11521 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
    11581 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
    11641 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
    11701 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
    11761 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
    11821 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
    11881 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    11941 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    12001 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
    12061 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
    12121 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    12181 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    12241 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
    12301 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    12361 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
    12421 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
    12481 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    12541 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    12601 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    12661 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    12721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    12781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    12841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    12901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    12961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    13021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    13081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    13141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    13201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    13261 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    13321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    13381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    13441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    13501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    13561 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    13621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    13681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    13741 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    13801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    13861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    13921 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    13981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    14041 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    14101 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    14161 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    14221 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    14281 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    14341 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    14401 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    14461 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    14521 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    14581 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    14641 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    14701 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    14761 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    14821 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    14881 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    14941 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    15001 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    15061 cagtgccaag ctg
//
