LOCUS       V025548                11850 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025548
VERSION     V025548
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..6480
                     /label="VCPIP1(NM_025054)"
                     /note="VCPIP1(NM_025054)"
                     /gene="VCPIP1"
     CDS             6487..6516
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     misc_feature    6553..7139
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             7159..7758
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7772..8360
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8567..9200
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9328..9344)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9352..9368
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9376..9406)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9421..9442
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9730..10318)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10489..11349)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11350..11454)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11502..11636
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgtct
     2821 cagccgccgc cgccgccgcc tccgttgccg ccgccacctc ctccccctga ggctccacag
     2881 actccgtcgt ccttggcgtc ggcggctgct tcgggggggc ttttgaagcg gagagaccgg
     2941 agaatccttt ccgggagctg cccggatccg aagtgtcagg cgcgtctatt tttcccggcc
     3001 tccggttctg tcagcatcga gtgtaccgag tgcggccagc ggcacgagca gcaacagctg
     3061 ctgggggttg aggaggtgac cgacccggac gtagtgctac acaacctgct gcggaacgcg
     3121 ctgctcgggg ttacgggggc acccaagaag aacacggaac tggtaaaggt gatgggcctt
     3181 tccaactatc actgcaaatt gttgtcgccc atattagctc gctatggaat ggacaaacag
     3241 acaggccggg ccaagcttct ccgggacatg aaccagggcg aactgttcga ttgcgcctta
     3301 ctgggtgacc gcgccttcct catagaacca gagcatgtta acactgtggg ctatggcaag
     3361 gaccgctccg gaagcctcct gtatttgcat gacactctgg aggacattaa gcgggccaat
     3421 aaaagccagg aatgtctcat tccagtgcat gtggacgggg atggacactg cttggtgcat
     3481 gctgtgtctc gggctctagt aggccgagag ctcttctggc atgccttaag agagaatctt
     3541 aaacagcact ttcagcagca cctggcccga tatcaagctc tgttccatga cttcattgat
     3601 gctgctgagt gggaggacat tatcaatgag tgtgaccctc tgtttgtacc acctgagggt
     3661 gttcccttgg gcctgaggaa tatccacata tttggtcttg ccaatgtgct acatcgtcct
     3721 attattctgt tagattccct cagtggcatg agaagctctg gtgattattc agccaccttt
     3781 ctacctgggc tcatccctgc agagaagtgc actgggaaag atggtcattt gaacaaacca
     3841 atctgtattg catggagcag ctccggtaga aaccattata tccccttggt aggcataaaa
     3901 ggggctgctt tgcccaaact gcctatgaat ttgcttccta aagcatgggg tgtgcctcag
     3961 gaccttatta aaaagtacat aaaacttgaa gaggatggtg gttgtgttat tggaggtgac
     4021 agaagtttgc aagataaata cttacttagg cttgttgctg ctatggaaga agtctttatg
     4081 gacaaacatg gtatccatcc tagtttggtt gctgatgtcc atcagtattt ctacagaagg
     4141 actggagtga taggagttca gcctgaggaa gtcacagcag ctgctaaaaa agcagtaatg
     4201 gataatcgcc ttcacaaatg tttgctctgt ggtgcccttt ctgaacttca tgttcctcca
     4261 gagtggttgg ctcctggagg gaaattgtat aacctggcaa aaagtactca tggacagctg
     4321 aggactgaca aaaattacag ctttcccttg aacaatttgg tttgctcata tgattcagtg
     4381 aaagatgttc tggtaccaga ctatggaatg agtaacctaa cagcttgtaa ttggtgccat
     4441 ggcacatctg tgcgaaaggt cagaggagat gggtctattg tgtatttgga tggagacaga
     4501 actaattcta ggtccactgg tggcaaatgt ggttgtggat tcaaacactt ttgggatggt
     4561 aaggagtatg acaatctacc agaagctttc cctattactt tagaatgggg tggaagagtg
     4621 gtcagagaaa cagtatattg gttccagtat gaaagtgatt catctttgaa tagtaatgtt
     4681 tacgatgttg caatgaaact tgttaccaag cactttccag gtgaatttgg gagtgaaatc
     4741 ctagttcaga aagttgtcca cactatattg catcagactg ccaaaaagaa tcccgatgat
     4801 tatactcctg taaatataga tggtgctcac gcccaaagag ttggagatgt tcaaggacaa
     4861 gaatcagagt ctcagctccc aactaaaatt attcttactg gacagaaaac aaaaactttg
     4921 cacaaggagg agttaaacat gagtaaaact gaaagaacta ttcaacagaa tattacggaa
     4981 caggcttctg taatgcagaa acggaaaaca gagaagttaa aacaagaaca aaaagggcaa
     5041 cccaggactg tttctcccag taccattcgt gatggtccat cctctgcacc tgctacacct
     5101 accaaggctc cctattcacc gacaacttct aaggagaaga agatccgaat cacaactaat
     5161 gatggacgac agtccatggt tacccttaag tcttcaacaa ccttttttga acttcaggaa
     5221 agtatagcca gagaattcaa cattcctcca tatttacagt gtattcgata cgggtttcct
     5281 cctaaagagt taatgccacc acaggcagga atggaaaagg aaccagttcc tttacagcat
     5341 ggcgacagaa ttacaataga aattctaaaa agtaaagctg aaggtggtca gtctgctgca
     5401 gcacactcag cccacactgt gaaacaagaa gatattgctg ttactggtaa actgtcatct
     5461 aaggaacttc aggagcaagc tgaaaaagaa atgtactcct tgtgtctttt agcaacatta
     5521 atgggagaag atgtatggtc ttatgcaaag ggacttcctc acatgtttca gcagggtggt
     5581 gtattctaca gtattatgaa gaaaaccatg ggtatggctg atggcaagca ttgtactttt
     5641 ccacatctgc ctggcaaaac ctttgtctat aatgcttctg aagatagact ggaattgtgt
     5701 gtggatgctg caggacattt ccccattggt cctgatgttg aagatttagt taaagaggct
     5761 gtaagtcagg ttcgagcaga ggctactaca agaagtaggg aatcaagtcc ctcacatggg
     5821 ctattaaaac taggtagtgg tggagtagtg aaaaagaaat ctgagcaact tcataacgta
     5881 actgcctttc agggaaaagg gcattcttta ggaactgcat ctggtaaccc acaccttgat
     5941 ccaagagcta gggaaacttc agttgtaaga aagcataata cagggacaga ctttagtaat
     6001 agttccacta aaacagagcc ttctgtattc acagcttctt ctagtaatag tgagcttatt
     6061 cgaatagctc ctggagtagt aacaatgaga gacggcaggc agcttgatcc tgatttggtt
     6121 gaggcccagc gaaaaaaatt gcaggaaatg gtttcttcta ttcaggcttc aatggacagg
     6181 caccttcggg atcaaagtac agagcagtca ccatctgatc ttcctcaaag gaaaacagaa
     6241 gttgtgagtt cttctgcaaa gtctgggagt cttcagactg gtttgcctga atcttttcct
     6301 ttaactggtg gtactgaaaa tttgaataca gaaacaactg atggctgtgt agcagatgca
     6361 ctgggagcag cctttgccac aaggtcaaaa gcacaaaggg gaaattccgt ggaggagctt
     6421 gaagagatgg atagtcaaga tgctgagatg actaacacaa ctgagccaat ggatcactct
     6481 ctcgaggaac aaaaactcat ctcagaagag gatctgtgat cgagactagt tctagagcgg
     6541 ccgcggatcc cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     6601 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     6661 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     6721 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     6781 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     6841 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     6901 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     6961 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     7021 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     7081 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc
     7141 acaaggagac gaccttccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     7201 gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     7261 cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7321 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7381 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7441 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7501 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7561 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7621 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7681 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7741 cgcaagcccg gtgcctagac gcgtctggaa caatcaacct ctggattaca aaatttgtga
     7801 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     7861 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     7921 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7981 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     8041 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     8101 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     8161 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     8221 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     8281 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     8341 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     8401 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     8461 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     8521 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     8581 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     8641 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     8701 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     8761 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     8821 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     8881 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     8941 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     9001 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9061 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9121 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9181 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     9241 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac
     9301 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9361 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9421 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9541 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9601 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9661 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9721 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9781 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9841 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9901 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9961 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10021 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10081 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10141 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    10201 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10261 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10321 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10381 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10441 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10501 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10561 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10621 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10681 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10741 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10801 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10861 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10921 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10981 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11041 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11101 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11161 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11221 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11281 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11341 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11461 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt
    11521 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    11581 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    11641 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    11701 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11761 aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat
    11821 ttgttaaata tgtactacaa acttagtagt
//
