LOCUS V046090 10555 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046090 VERSION V046090 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" regulatory 2809..2818 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 2818..2847 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2848..5226 /label="Ufl1(NM_026194)" /note="Ufl1(NM_026194)" /gene="Ufl1" misc_feature 5258..5844 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES2" CDS 5864..6463 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6477..7065 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7272..7905 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(8033..8049) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8057..8073 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8081..8111) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8126..8147 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8435..9023) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9194..10054) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10055..10159) /gene="bla" /label="AmpR promoter" polyA_signal 10207..10341 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggag 2821 cagaagctga tctcagagga ggacctgatg gcggacgcct gggaggagat ccggcggctg 2881 gcggctgact tccagcgggc gcagtttgca gagtccacgc aaaggttgtc agagcggaac 2941 tgcattgaga ttgtgaataa attgatctct cagaaacagc tggaagtagt ccatacactt 3001 gacggcaagg aatatatcac tccagcccaa atcagtaaag aaatgagaga cgagctacat 3061 gtccgaggtg gtcgagtaaa cattgttgac ttacaacagg ttattaatgt ggacctgact 3121 catatcgaga gtagagtcag tgatattatt aaatcagaga agcacgtgca gatggtactg 3181 ggacaactga ttgatgagaa ctatctggat cagttatcag aagaggtgaa tgataaattg 3241 caagaaagtg gtcaagtaac tgtctcagaa ctgtgtaaag cctatgacct tcctggggat 3301 tttctgacac aggcattaac tcagcgactt ggccggatta tcaatggcca tctggacctt 3361 gataacagag gagtgatttt cactgaggcc tttgtagctc gccataaagc acgtatccgc 3421 ggactcttca gtgccattac acggcctaca ccagtgaact ctttggtttc aaaatatgga 3481 tttcaggaac agctccttta ctctgtgctc gaggacctcg tcagcactgg ccgcttacga 3541 ggcaccgtgg tcggcgggcg acaggataaa gcagtctttg tccctgacat ctactccagg 3601 acacagagta cttgggtgga ctcctttttc aggcagaatg gctatctaga atttgacgct 3661 ttatccagac ttggaatccc agatgctgtg aactatataa agaaaaggta taagaatacc 3721 caactcttgt ttttgaaggc aacttgtgtt ggccaaggac tcgtagacca ggtggaagca 3781 tccgtagagg aagccatcag ctcagggacc tgggttgaca tttcgcctct gctacccagt 3841 tccttgtcag ttgaagatgc tgctatgctg ctccagcagg tgatgagacc attcggcaag 3901 ctcgcttcag ctatagtctt tagtgacacg gttgtggtga gtgaaaagtt cataactgac 3961 tgcacaggac tgttcagcga acggatgcac caaaaagctg agaaggaaat gaaaaataat 4021 cctgtgcatt taatcactga agaagatctg aaacaaattt ccattttaga aagtgttaat 4081 acaagtaaaa aggataaaaa agatgaacgc aggaagaaag caacagaggg ctctggaagt 4141 gtaagaggag gaggaggggg caatgccaga gaatataaaa ttaagaaaac caagaagaag 4201 ggaagaaaag atgaggacag tgatgatgag tcccagtcgt cgcatggtgg aaagaagaag 4261 ccggacatca cattcatgtt ccaggatgaa attgaagatt gcttaaggaa acacattcag 4321 gatgcccctg aggagtttat ttcagagctt gcagaatact taataaagcc tcttaataaa 4381 atgtatcttg aggtagtacg ttcagtcttc atgtcttcaa catctgcttc tggaactggc 4441 agaaaacgca caatcaagga cttacaagaa gaggtttcaa acctatacaa taatatcaga 4501 ttatttgaaa agggaatgaa atattttgca gatgacacac agactgctct taccaagcac 4561 ttgctgaaga cagtgtgtac cgatatcact aacctcatgt tcaacttcct agcctcagat 4621 ttcctcatgg cagtggagga gcctgcagcc attacaagtg atataagaaa aaaaatttta 4681 agcaaattga cagaagaaac caaagtagct ctcacaaaac tccataactc tctcaatgaa 4741 aaaagcatag aagacttcct gtcttgcctg gattctgcca cagaagcgtg tgacattatg 4801 gtgaaaaagg gagacaagaa aagggaaaga caaatcctct tccagcatcg acaggctctg 4861 tgtgagcagc tgaaggtcac agaagaccct gctctgattt tacacctcac agcagtcctg 4921 ttatttcagc tctcaaccca cagcatgctc cacgcacctg gaaggtgtgt cccacagatc 4981 atcgcgtttc ttcacagcaa aatcccagag gatcaacata ctctcttggt aaaatatcaa 5041 ggtttggttg tgaaacagtt agtcagtcaa aataagaaga ctgggcaggg agaggatccg 5101 tcaagcgatg aattagacaa ggaacaacat gatgtcacaa acgctactcg gaaagagctt 5161 caggaactgt cattatccat taaggacctt gttctcaagt ccaggaaatc atctgtcaca 5221 gaggagtaaa ctagttctag agcggccgcg gatcccgccc ctctccctcc ccccccccta 5281 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt 5341 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga 5401 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg 5461 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt 5521 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat 5581 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg 5641 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg 5701 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt 5761 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa 5821 cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg agtacaagcc 5881 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc 5941 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt 6001 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 6061 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 6121 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 6181 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 6241 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 6301 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 6361 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 6421 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tagacgcgtc tggaacaatc 6481 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 6541 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 6601 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 6661 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 6721 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 6781 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 6841 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 6901 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 6961 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 7021 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg 7081 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc 7141 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca 7201 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 7261 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg 7321 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt 7381 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt 7441 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat 7501 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca 7561 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa 7621 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg 7681 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag 7741 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 7801 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 7861 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 7921 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt 7981 gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 8041 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 8101 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 8161 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 8221 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 8281 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 8341 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 8401 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 8461 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 8521 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 8581 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 8641 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 8701 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 8761 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 8821 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 8881 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 8941 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 9001 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 9061 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 9121 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 9181 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 9241 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 9301 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 9361 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 9421 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 9481 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 9541 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 9601 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 9661 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 9721 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 9781 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 9841 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 9901 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 9961 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 10021 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 10081 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 10141 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 10201 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 10261 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 10321 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt 10381 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt 10441 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac 10501 ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt //