LOCUS       V026306                10317 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026306
VERSION     V026306
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..4947
                     /label="USP51(NM_201286)"
                     /note="USP51(NM_201286)"
                     /gene="USP51"
     CDS             4954..4983
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     misc_feature    5020..5606
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             5626..6225
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6239..6827
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7034..7667
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7795..7811)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7819..7835
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7843..7873)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7888..7909
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8197..8785)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8956..9816)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9817..9921)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9969..10103
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggcc
     2821 caggttcgag aaacttcttt gccctccggc tctggggtcc gctggatctc cggaggtggg
     2881 ggaggagcct ctcctgagga ggcggttgag aaggcgggga aaatggagga ggcggcggcg
     2941 ggggctacga aggcgtcttc gagacgtgaa gccgaggaga tgaagctgga gccattacaa
     3001 gagcgtgagc ccgcgccgga ggagaacttg acgtggagca gcagcggcgg cgacgagaag
     3061 gtgctccctt caatccccct tcgctgtcac agcagctcct cgcccgtttg cccgcgccgc
     3121 aagccccgcc ctcggcccca gccccgggcc cgctcccgca gccagcctgg gctctcggcc
     3181 ccacccccgc ctccagcccg gcccccgccc ccgccgccac ccccgccccc acccgcaccg
     3241 cggcccaggg cctggcgtgg atcccggcgc agatcccggc ctgggtccag gcctcagaca
     3301 cggagaagct gctctggtga cctagacggg tcgggggatc ctggcggctt aggggactgg
     3361 ttgctggagg tcgagtttgg tcagggtccc acaggctgct ctcatgtgga gagctttaaa
     3421 gtaggtaaga actggcagaa gaacctgagg ttgatctacc agcgtttcgt ttggagtggg
     3481 accccagaga ctaggaaacg taaagcaaag tcatgcatct gtcacgtatg tagtacccat
     3541 atgaacagac tccactcttg tctctcctgt gtcttttttg gctgcttcac tgagaaacat
     3601 attcacaaac atgcagaaac aaagcagcac catttagctg tagaccttta tcatggggtc
     3661 atatattgct tcatgtgtaa ggattatgta tatgacaaag acatagaaca gattgccaaa
     3721 gaaacaaaag aaaaaatttt gagattatta acttccacct caacagatgt ttctcatcaa
     3781 cagtttatga catcagggtt tgaagacaag caatcaacct gtgagacaaa ggaacaggag
     3841 ccaaaattgg tgaaacccaa gaaaaagaga agaaaaaagt cagtctatac tgtaggcctg
     3901 agagggctaa tcaatcttgg gaacacttgt tttatgaatt gtattgtcca ggcacttacc
     3961 catattcctc tactgaaaga tttcttcctc tctgacaagc acaaatgtat aatgacaagc
     4021 cccagcttgt gtctggtctg tgaaatgtct tcgctttttc atgctatgta ctctgggagc
     4081 cgaactcctc acattcccta taagttactg catctgatat ggatccatgc agaacattta
     4141 gcagggtaca ggcagcagga tgcccatgag ttccttattg caatattaga cgtgctacat
     4201 agacacagca aagatgatag tggtgggcag gaggccaata accccaactg ctgtaactgc
     4261 atcatagacc aaatctttac aggtggcctg caatcagatg tcacatgtca agcctgccat
     4321 agtgtttcta ccaccataga cccatgctgg gacatcagtt tggacttgcc tggctcttgt
     4381 gccacattcg attcccagaa cccagagagg gctgacagca cagtgagcag ggatgaccac
     4441 ataccaggaa tcccctcact tacagactgt ctacagtggt ttacaaggcc agagcaccta
     4501 ggaagcagtg ccaaaatcaa atgcaatagt tgccaaagct accaggagtc tactaaacag
     4561 ctcacaatga aaaaattacc cattgtggct tgttttcatc tcaagcggtt tgagcatgta
     4621 ggcaaacaga ggcgaaagat taataccttt atctcctttc ccttggagct ggacatgact
     4681 ccgtttttgg cctctactaa agagagcaga atgaaagaag gccagccacc aacagattgt
     4741 gtgcccaatg agaataagta ttccttgttt gcagtgatta atcaccatgg aactttggaa
     4801 agtggccact ataccagctt catccggcaa caaaaggacc agtggttcag ctgtgatgat
     4861 gccatcatca ccaaggctac cattgaggac ttactctaca gtgaagggta tttactgttc
     4921 tatcacaaac agggtctaga gaaagacctc gaggaacaaa aactcatctc agaagaggat
     4981 ctgtgatcga gactagttct agagcggccg cggatcccgc ccctctccct cccccccccc
     5041 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     5101 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     5161 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     5221 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     5281 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     5341 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     5401 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     5461 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     5521 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     5581 aacacgatga taagcttgcc acaacccaca aggagacgac cttccatgac cgagtacaag
     5641 cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc
     5701 gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg
     5761 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5821 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5881 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5941 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6001 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6061 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6121 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6181 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctagacgcg tctggaacaa
     6241 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6301 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6361 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6421 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6481 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6541 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6601 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     6661 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6721 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6781 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc
     6841 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     6901 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     6961 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7021 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     7081 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     7141 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     7201 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     7261 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     7321 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     7381 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     7441 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     7501 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7561 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7621 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7681 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     7741 ttgacattgc tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7801 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7861 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7921 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7981 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8041 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8101 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8161 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8221 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8281 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8341 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8401 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8461 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8521 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8581 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8641 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8701 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8761 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8821 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8881 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8941 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9001 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9061 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9121 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9181 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9241 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9301 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9361 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9421 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9481 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9541 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9601 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9661 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9721 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9781 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9841 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9901 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     9961 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    10021 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    10081 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    10141 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
    10201 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    10261 acttagtagt ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
