LOCUS       V023587                11028 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023587
VERSION     V023587
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..5640
                     /label="USP33(NM_015017)"
                     /note="USP33(NM_015017)"
                     /gene="USP33"
     CDS             5647..5712
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    5731..6317
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             6337..6936
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6950..7538
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7745..8378
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8506..8522)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8530..8546
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8554..8584)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8599..8620
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8908..9496)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9667..10527)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10528..10632)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10680..10814
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgaca
     2821 ggatcaaatt cacacataac gatattaacc ttaaaggtgt tacctcattt tgaaagtctt
     2881 gggaaacagg aaaaaattcc taacaaaatg tcagcttttc gaaatcattg tccacatttg
     2941 gattcagttg gtgaaataac aaaagaagat ttgatacaaa aatcccttgg tacttgtcag
     3001 gattgtaaag tccaaggacc aaatctttgg gcatgtctgg agaatagatg ttcatatgtt
     3061 ggctgtggtg aatcacaagt agatcacagc accatacatt ctcaggagac aaagcattat
     3121 ctaactgtga accttaccac tcttcgagta tggtgttatg cttgcagcaa agaagtattt
     3181 ttggatagga aattaggaac tcagccttca ttgcctcatg taagacaacc tcaccaaata
     3241 caagaaaaca gtgtccagga ttttaaaata cccagtaata caacattaaa aactcctctg
     3301 gttgccgtat ttgatgatct ggatatagaa gcggatgaag aagatgaact tagggccaga
     3361 ggtcttacag gtttgaaaaa tattggaaat acttgttaca tgaatgcagc tttgcaggct
     3421 ctttctaatt gcccaccttt gacacagttt tttcttgatt gtggaggact agctcgaaca
     3481 gataagaaac ctgccatttg taaaagttat ctcaaactaa tgacagagct gtggcataaa
     3541 agcaggccag gatctgttgt gcctactact ctgtttcaag gaattaaaac tgtaaatcca
     3601 acatttcggg ggtattctca gcaggatgct caagaattcc ttcgatgttt aatggatttg
     3661 cttcatgaag aattgaaaga gcaagtcatg gaagtagaag aagatccgca aaccataacc
     3721 actgaggaga caatggaaga agacaagagc cagtcggatg tagattttca gtcttgtgaa
     3781 tcttgtagca acagtgatag agcagaaaat gaaaatggct ctagatgctt ttctgaagat
     3841 aataatgaaa caacaatgtt aattcaggat gatgaaaaca attcagaaat gtcaaaggat
     3901 tggcaaaaag agaagatgtg caataagatt aataaagtaa attctgaagg cgaatttgat
     3961 aaagatagag actctatatc tgaaacagtc gacttaaaca accaggaaac tgtcaaagtg
     4021 caaatacaca gcagagcttc agaatatatc actgatgtcc attcgaatga cctgtctaca
     4081 ccacagatcc ttccatcaaa tgaaggtgtt aatccacgtt tatcggcaag ccctcctaaa
     4141 tcaggcaatt tgtggccagg attggcacca ccacacaaaa aagctcagtc tgcatctcca
     4201 aagagaaaaa aacagcacaa gaaatacaga agtgttattt cagacatatt tgatggaaca
     4261 atcattagtt cagtgcagtg tctgacttgt gacagggtgt ctgtaaccct cgagaccttt
     4321 caagatctgt ccttgccaat tcctggcaag gaagaccttg ctaagctgca ttcatcaagt
     4381 catccaactt ctatagtcaa agcaggatca tgtggcgaag catatgctcc acaagggtgg
     4441 atagcttttt tcatggaata tgtgaagagg tttgttgtct catgtgtccc tagctggttt
     4501 tggggtccag tagtaacctt gcaagattgt cttgctgcct tctttgccag agatgaacta
     4561 aaaggtgaca atatgtacag ttgtgaaaaa tgcaaaaagt tgagaaatgg agtgaagttt
     4621 tgtaaagtac aaaactttcc tgagattttg tgcatccacc ttaaaagatt cagacatgaa
     4681 ctaatgtttt ccaccaaaat cagtacccat gtttcatttc cgctagaagg cttggatctt
     4741 cagccatttc ttgctaagga tagtccagct caaattgtga catatgatct tctgtcagtc
     4801 atttgccatc atggaactgc aagtagtgga cactatatag cctactgccg aaacaatcta
     4861 aataatctct ggtatgaatt tgatgatcag agtgtcactg aagtttcaga atctactgta
     4921 caaaatgcag aagcttacgt tcttttctat aggaagagca gcgaagaggc acaaaaagag
     4981 aggagaagga tatcaaattt attgaacata atggaaccaa gcctccttca gttttatatt
     5041 tctcgacagt ggcttaataa atttaagacc tttgccgaac ctggccctat ttcaaataat
     5101 gactttcttt gtattcatgg aggtgttcct ccaagaaaag ctggttatat tgaagacctg
     5161 gttttgatgc tgcctcagaa catttgggat aacctatata gcaggtatgg tggaggacca
     5221 gctgtcaacc atctgtacat ttgtcatact tgccaaattg aggcggagaa aattgaaaaa
     5281 agaagaaaaa ctgaattgga aatttttatt cggcttaaca gagcgttcca aaaagaggac
     5341 tctccagcta ctttttattg catcagtatg cagtggttta gagaatggga aagttttgtg
     5401 aagggtaaag atggagatcc tccaggtcct attgacaata ctaagattgc agtcactaaa
     5461 tgtggtaatg tgatgcttag gcaaggagca gattctggcc agatttctga agaaacatgg
     5521 aattttctgc agtctattta tggtggaggg cctgaagtta tcctgcgacc tccggttgtt
     5581 catgttgatc cagatatact tcaagcagaa gaaaaaattg aagtagaaac tcggtctttg
     5641 ctcgaggact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     5701 gacgatgaca agtagcggcc gcggatcccg cccctctccc tccccccccc ctaacgttac
     5761 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     5821 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     5881 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     5941 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     6001 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     6061 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     6121 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     6181 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     6241 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     6301 ataagcttgc cacaacccac aaggagacga ccttccatga ccgagtacaa gcccacggtg
     6361 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc
     6421 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag
     6481 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     6541 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     6601 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     6661 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     6721 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6781 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6841 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     6901 cgcacctggt gcatgacccg caagcccggt gcctagacgc gtctggaaca atcaacctct
     6961 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7021 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7081 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7141 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7201 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7261 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7321 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     7381 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7441 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7501 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga
     7561 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     7621 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     7681 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg
     7741 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     7801 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     7861 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     7921 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     7981 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     8041 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     8101 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     8161 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     8221 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8281 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8341 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8401 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg
     8461 ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     8521 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8581 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8641 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8701 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8761 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8821 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8881 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8941 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9001 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9061 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9121 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9181 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9241 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9301 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     9361 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9421 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9481 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9541 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9601 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9661 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9721 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9781 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9841 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9901 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9961 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    10021 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10081 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10141 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10201 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10261 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10321 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10381 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10441 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10501 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10561 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10621 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca
    10681 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10741 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10801 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    10861 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    10921 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    10981 tttttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
