LOCUS       V026253                 9774 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026253
VERSION     V026253
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..4404
                     /label="USP17L24(NM_001242327)"
                     /note="USP17L24(NM_001242327)"
                     /gene="USP17L24"
     CDS             4411..4440
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     misc_feature    4477..5063
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             5083..5682
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5696..6284
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6491..7124
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7252..7268)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7276..7292
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7300..7330)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7345..7366
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7654..8242)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8413..9273)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9274..9378)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9426..9560
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggag
     2821 gacgactcac tctacttgag aggtgagtgg cagttcaacc acttttcaaa actcacatct
     2881 tctcggcccg atgcagcttt tgctgaaatc cagcggactt ctctccctga gaagtcacca
     2941 ctctcatgtg agacccgtgt cgacctctgt gatgatttgg ctcctgtggc aagacagctt
     3001 gctcccaggg agaagcttcc tctgagtagc aggagacctg ctgcggtggg ggctgggctc
     3061 cagaatatgg gaaatacctg ctacgtgaac gcttccttgc agtgcctgac atacacaccg
     3121 ccccttgcca actacatgct gtcccgggag cactctcaaa cgtgtcatcg tcacaagggc
     3181 tgcatgctct gtactatgca agctcacatc acacgggccc tccacaatcc tggccacgtc
     3241 atccagccct cacaggcatt ggctgctggc ttccatagag gcaagcagga agatgcccat
     3301 gaatttctca tgttcactgt ggatgccatg aaaaaggcat gccttcccgg gcacaagcag
     3361 gtagatcatc actctaagga caccaccctc atccaccaaa tatttggagg ctactggaga
     3421 tctcaaatca agtgtctcca ctgccacggc atttcagaca cttttgaccc ttacctggac
     3481 atcgccctgg atatccaggc agctcagagt gtccagcaag ctttggaaca gttggtgaag
     3541 cccgaagaac tcaatggaga gaatgcctat cattgtggtg tttgtctcca gagggcgccg
     3601 gcctccaaga cgttaacttt acacacctct gccaaggtcc tcatccttgt attgaagaga
     3661 ttctccgatg tcacaggcaa caagattgcc aagaatgtgc aatatcctga gtgccttgac
     3721 atgcagccat acatgtctca gccgaacaca ggacctctcg tctatgtcct ctatgctgtg
     3781 ctggtccacg ctgggtggag ttgtcacaac ggacattact tctcttatgt caaagctcaa
     3841 gaaggccagt ggtataaaat ggatgatgcc gaggtcaccg cctctagcat cacttctgtc
     3901 ctgagtcaac aggcctacgt cctcttttac atccagaaga gtgaatggga aagacacagt
     3961 gagagtgtgt caagaggcag ggaaccaaga gcccttggcg cagaagacac cgacaggcga
     4021 gcaacgcaag gagagctcaa gagagaccac ccctgcctcc aggcccccga gttggacgag
     4081 cacttggtgg aaagagccac tcaggaaagc accttagacc actggaaatt ccttcaagag
     4141 caaaacaaaa cgaagcctga gttcaacgtc agaaaagtcg aaggtaccct gcctcccgac
     4201 gtacttgtga ttcatcaatc aaaatacaag tgtgggatga agaaccatca tcctgaacag
     4261 caaagctccc tgctaaacct ctcttcgtcg accccgacac atcaggagtc catgaacact
     4321 ggcacactcg cttccctgcg agggagggcc aggagatcca aagggaagaa caaacacagc
     4381 aagagggctc tgcttgtgtg ccagctcgag gaacaaaaac tcatctcaga agaggatctg
     4441 tgatcgagac tagttctaga gcggccgcgg atcccgcccc tctccctccc ccccccctaa
     4501 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     4561 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     4621 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     4681 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     4741 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     4801 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     4861 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     4921 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     4981 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     5041 acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga gtacaagccc
     5101 acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg
     5161 ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc
     5221 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     5281 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     5341 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     5401 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     5461 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     5521 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     5581 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     5641 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct agacgcgtct ggaacaatca
     5701 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5761 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5821 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5881 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5941 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6001 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6061 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     6121 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6181 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6241 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc
     6301 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     6361 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     6421 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6481 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     6541 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     6601 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     6661 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     6721 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     6781 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     6841 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     6901 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     6961 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7021 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7081 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7141 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg
     7201 acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     7261 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7321 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7381 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7441 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7501 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7561 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7621 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7681 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7741 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7801 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7861 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7921 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7981 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8041 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     8101 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8161 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8221 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8281 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8341 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8401 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8461 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8521 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8581 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8641 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8701 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8761 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8821 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8881 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8941 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9001 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9061 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9121 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9181 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9241 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9301 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9361 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     9421 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9481 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9541 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     9601 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     9661 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     9721 tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
