LOCUS V018407 9595 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018407 VERSION V018407 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" regulatory 2809..2818 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 2818..2847 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2854..4278 /label="TRIM21(NM_003141)" /note="TRIM21(NM_003141)" /gene="TRIM21" misc_feature 4298..4884 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES2" CDS 4904..5503 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5517..6105 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6312..6945 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7073..7089) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7097..7113 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7121..7151) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7166..7187 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7475..8063) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8234..9094) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9095..9199) /gene="bla" /label="AmpR promoter" polyA_signal 9247..9381 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggaa 2821 caaaaactca tctcagaaga ggatctgctc gagatggctt cagcagcacg cttgacaatg 2881 atgtgggagg aggtcacatg ccctatctgc ctggacccct tcgtggagcc tgtgagcatc 2941 gagtgtggcc acagcttctg ccaggaatgc atctctcagg ttgggaaagg tgggggcagc 3001 gtctgtcctg tgtgccggca gcgctttctg ctcaagaatc tccggcccaa tcgacagcta 3061 gccaacatgg tgaacaacct taaagaaatc agccaggagg ccagagaggg cacacagggg 3121 gaacggtgtg cagtgcatgg agagagactt cacctgttct gtgagaaaga tgggaaggcc 3181 ctttgctggg tatgtgccca gtctcggaaa caccgtgacc acgccatggt ccctcttgag 3241 gaggctgcac aggagtacca ggagaagctc caggtggcat taggggaact gagaagaaag 3301 caggagttgg ctgagaagtt ggaagtggaa attgcaataa agagagcaga ctggaagaaa 3361 acagtggaaa cacagaaatc taggattcac gcagagtttg tgcagcaaaa aaacttcctg 3421 gttgaagaag aacagaggca gctgcaggag ctggagaagg atgagaggga gcagctgaga 3481 atcctggggg agaaagaggc caagctggcc cagcagagcc aggccctaca ggagctcatc 3541 tcagagctag atcgaaggtg ccacagctca gcactggaac tgctgcagga ggtgataatt 3601 gtcctggaaa ggagtgagtc ctggaacctg aaggacctgg atattacctc tccagaactc 3661 aggagtgtgt gccatgtgcc agggctgaag aagatgctga ggacatgtgc agtccacatc 3721 actctggatc cagacacagc caatccgtgg ctgatacttt cagaagatcg gagacaagtg 3781 aggcttggag acacccagca gagcatacct ggaaatgaag agagatttga tagttatcct 3841 atggtcctgg gtgcccagca ctttcactct ggaaaacatt actgggaggt agatgtgaca 3901 ggaaaggagg cctgggacct gggtgtctgc agagactctg tgcgcaggaa ggggcacttt 3961 ttgcttagtt ccaagagtgg cttctggaca atttggttgt ggaacaaaca aaaatatgag 4021 gctggcacct acccccagac tcccctccac cttcaggtgc ctccatgcca agttgggatt 4081 ttcctggact atgaggctgg catggtctcc ttctacaaca tcactgacca tggctccctc 4141 atctactcct tctctgaatg tgcctttaca ggacctctgc ggcccttctt cagtcctggt 4201 ttcaatgatg gaggaaaaaa cacagcccct ctaaccctct gtccactgaa tattggatca 4261 caaggatcca ctgactattg agcggccgcg gatcccgccc ctctccctcc ccccccccta 4321 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt 4381 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga 4441 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg 4501 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt 4561 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat 4621 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg 4681 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg 4741 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt 4801 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa 4861 cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg agtacaagcc 4921 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc 4981 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt 5041 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 5101 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 5161 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 5221 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 5281 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 5341 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 5401 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 5461 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tagacgcgtc tggaacaatc 5521 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 5581 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 5641 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 5701 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 5761 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 5821 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 5881 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 5941 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 6001 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 6061 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg 6121 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc 6181 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca 6241 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 6301 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg 6361 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt 6421 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt 6481 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat 6541 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca 6601 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa 6661 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg 6721 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag 6781 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 6841 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 6901 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 6961 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt 7021 gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 7081 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 7141 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 7201 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 7261 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 7321 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 7381 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 7441 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 7501 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 7561 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 7621 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 7681 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7741 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7801 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7861 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7921 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7981 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 8041 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 8101 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 8161 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 8221 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 8281 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 8341 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 8401 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 8461 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 8521 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 8581 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 8641 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 8701 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8761 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8821 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8881 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8941 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 9001 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 9061 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 9121 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 9181 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 9241 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 9301 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 9361 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt 9421 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt 9481 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac 9541 ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt //