LOCUS       V046842                11923 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046842
VERSION     V046842
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2830..4323
                     /codon_start=1
                     /note="binds to CIB1 upon exposure to blue light (Kennedy
                     et al., 2010)"
                     /product="N-terminal photolyase homology region of
                     Arabidopsis thaliana cryptochrome 2"
                     /transl_table=1
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
                     /label="CRY2PHR"
     CDS             4348..5055
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5086..6606
                     /label="SLC7A5(NM_003486)"
                     /note="SLC7A5(NM_003486)"
                     /gene="SLC7A5"
     misc_feature    6626..7212
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             7232..7831
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7845..8433
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8640..9273
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9401..9417)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9425..9441
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9449..9479)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9494..9515
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9803..10391)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10562..11422)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11423..11527)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11575..11709
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcta gtccagtgtg
     2821 gtgctcgaga tgaagatgga caaaaagact atagtttggt ttagaagaga cctaaggatt
     2881 gaggataatc ctgcattagc agcagctgct cacgaaggat ctgtttttcc tgtcttcatt
     2941 tggtgtcctg aagaagaagg acagttttat cctggaagag cttcaagatg gtggatgaaa
     3001 caatcacttg ctcacttatc tcaatccttg aaggctcttg gatctgacct cactttaatc
     3061 aaaacccaca acacgatttc agcgatcttg gattgtatcc gcgttaccgg tgctacaaaa
     3121 gtcgtcttta accacctcta tgatcctgtt tcgttagttc gggaccatac cgtaaaggag
     3181 aagctggtgg aacgtgggat ctctgtgcaa agctacaatg gagatctatt gtatgaaccg
     3241 tgggagatat actgcgaaaa gggcaaacct tttacgagtt tcaattctta ctggaagaaa
     3301 tgcttagata tgtcgattga atccgttatg cttcctcctc cttggcggtt gatgccaata
     3361 actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag aactagggct ggagaatgag
     3421 gccgagaaac cgagcaatgc gttgttaact agagcttggt ctccaggatg gagcaatgct
     3481 gataagttac taaatgagtt catcgagaag cagttgatag attatgcaaa gaacagcaag
     3541 aaagttgttg ggaattctac ttcactactt tctccgtatc tccatttcgg ggaaataagc
     3601 gtcagacacg ttttccagtg tgcccggatg aaacaaatta tatgggcaag agataagaac
     3661 agtgaaggag aagaaagtgc agatcttttt cttaggggaa tcggtttaag agagtattct
     3721 cggtatatat gtttcaactt cccgtttact cacgagcaat cgttgttgag tcatcttcgg
     3781 tttttccctt gggatgctga tgttgataag ttcaaggcct ggagacaagg caggaccggt
     3841 tatccgttgg tggatgccgg aatgagagag ctttgggcta ccggatggat gcataacaga
     3901 ataagagtga ttgtttcaag ctttgctgtg aagtttcttc tccttccatg gaaatgggga
     3961 atgaagtatt tctgggatac acttttggat gctgatttgg aatgtgacat ccttggctgg
     4021 cagtatatct ctgggagtat ccccgatggc cacgagcttg atcgcttgga caatcccgcg
     4081 ttacaaggcg ccaaatatga cccagaaggt gagtacataa ggcaatggct tcccgagctt
     4141 gcgagattgc caactgaatg gatccatcat ccatgggacg ctcctttaac cgtactcaaa
     4201 gcttctggtg tggaactcgg aacaaactat gcgaaaccca ttgtagacat cgacacagct
     4261 cgtgagctac tagctaaagc tatttcaaga acccgtgaag cacagatcat gatcggagca
     4321 gcagcccggg atccaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
     4381 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     4441 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     4501 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     4561 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     4621 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     4681 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     4741 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     4801 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     4861 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     4921 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     4981 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     5041 gacgagctgt acaagggtgg cggttcgggc ggtggttcgt ctagaatggc gggtgcgggc
     5101 ccgaagcggc gcgcgctagc ggcgccggcg gccgaggaga aggaagaggc gcgggagaag
     5161 atgctggccg ccaagagcgc ggacggctcg gcgccggcag gcgagggcga gggcgtgacc
     5221 ctgcagcgga acatcacgct gctcaacggc gtggccatca tcgtggggac cattatcggc
     5281 tcgggcatct tcgtgacgcc cacgggcgtg ctcaaggagg caggctcgcc ggggctggcg
     5341 ctggtggtgt gggccgcgtg cggcgtcttc tccatcgtgg gcgcgctctg ctacgcggag
     5401 ctcggcacca ccatctccaa atcgggcggc gactacgcct acatgctgga ggtctacggc
     5461 tcgctgcccg ccttcctcaa gctctggatc gagctgctca tcatccggcc ttcatcgcag
     5521 tacatcgtgg ccctggtctt cgccacctac ctgctcaagc cgctcttccc cacctgcccg
     5581 gtgcccgagg aggcagccaa gctcgtggcc tgcctctgcg tgctgctgct cacggccgtg
     5641 aactgctaca gcgtgaaggc cgccacccgg gtccaggatg cctttgccgc cgccaagctc
     5701 ctggccctgg ccctgatcat cctgctgggc ttcgtccaga tcgggaaggg tgatgtgtcc
     5761 aatctagatc ccaacttctc atttgaaggc accaaactgg atgtggggaa cattgtgctg
     5821 gcattataca gcggcctctt tgcctatgga ggatggaatt acttgaattt cgtcacagag
     5881 gaaatgatca acccctacag aaacctgccc ctggccatca tcatctccct gcccatcgtg
     5941 acgctggtgt acgtgctgac caacctggcc tacttcacca ccctgtccac cgagcagatg
     6001 ctgtcgtccg aggccgtggc cgtggacttc gggaactatc acctgggcgt catgtcctgg
     6061 atcatccccg tcttcgtggg cctgtcctgc ttcggctccg tcaatgggtc cctgttcaca
     6121 tcctccaggc tcttcttcgt ggggtcccgg gaaggccacc tgccctccat cctctccatg
     6181 atccacccac agctcctcac ccccgtgccg tccctcgtgt tcacgtgtgt gatgacgctg
     6241 ctctacgcct tctccaagga catcttctcc gtcatcaact tcttcagctt cttcaactgg
     6301 ctctgcgtgg ccctggccat catcggcatg atctggctgc gccacagaaa gcctgagctt
     6361 gagcggccca tcaaggtgaa cctggccctg cctgtgttct tcatcctggc ctgcctcttc
     6421 ctgatcgccg tctccttctg gaagacaccc gtggagtgtg gcatcggctt caccatcatc
     6481 ctcagcgggc tgcccgtcta cttcttcggg gtctggtgga aaaacaagcc caagtggctc
     6541 ctccagggca tcttctccac gaccgtcctg tgtcagaagc tcatgcaggt ggtcccccag
     6601 gagacatagg cggccgcgga tcccgcccct ctccctcccc cccccctaac gttactggcc
     6661 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     6721 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     6781 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     6841 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     6901 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     6961 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     7021 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     7081 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     7141 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag
     7201 cttgccacaa cccacaagga gacgaccttc catgaccgag tacaagccca cggtgcgcct
     7261 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
     7321 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
     7381 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     7441 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     7501 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     7561 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     7621 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     7681 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     7741 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     7801 ctggtgcatg acccgcaagc ccggtgccta gacgcgtctg gaacaatcaa cctctggatt
     7861 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     7921 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     7981 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     8041 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     8101 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     8161 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     8221 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     8281 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     8341 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     8401 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct
     8461 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     8521 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     8581 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact
     8641 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     8701 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     8761 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     8821 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     8881 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     8941 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     9001 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     9061 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     9121 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     9181 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     9241 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     9301 agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc
     9361 gtttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     9421 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     9481 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     9541 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     9601 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9661 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9721 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9781 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9841 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9901 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9961 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    10021 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    10081 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    10141 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    10201 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    10261 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    10321 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    10381 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    10441 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    10501 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    10561 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    10621 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    10681 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    10741 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10801 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10861 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10921 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10981 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    11041 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    11101 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    11161 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    11221 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    11281 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    11341 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    11401 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    11461 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    11521 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg
    11581 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    11641 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    11701 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    11761 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    11821 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttttt
    11881 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
