LOCUS V022710 9522 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022710 VERSION V022710 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2815..4176 /label="MYC(NM_002467)" /note="MYC(NM_002467)" /gene="MYC" CDS 4177..4194 /codon_start=1 /product="6xHis affinity tag" /transl_table=1 /translation="HHHHHH" /label="6xHis" misc_feature 4225..4811 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES2" CDS 4831..5430 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5444..6032 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6239..6872 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7000..7016) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7024..7040 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7048..7078) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7093..7114 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7402..7990) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8161..9021) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9022..9126) /gene="bla" /label="AmpR promoter" polyA_signal 9174..9308 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggat 2821 ttttttcggg tagtggaaaa ccagcagcct cccgcgacga tgcccctcaa cgttagcttc 2881 accaacagga actatgacct cgactacgac tcggtgcagc cgtatttcta ctgcgacgag 2941 gaggagaact tctaccagca gcagcagcag agcgagctgc agcccccggc gcccagcgag 3001 gatatctgga agaaattcga gctgctgccc accccgcccc tgtcccctag ccgccgctcc 3061 gggctctgct cgccctccta cgttgcggtc acacccttct cccttcgggg agacaacgac 3121 ggcggtggcg ggagcttctc cacggccgac cagctggaga tggtgaccga gctgctggga 3181 ggagacatgg tgaaccagag tttcatctgc gacccggacg acgagacctt catcaaaaac 3241 atcatcatcc aggactgtat gtggagcggc ttctcggccg ccgccaagct cgtctcagag 3301 aagctggcct cctaccaggc tgcgcgcaaa gacagcggca gcccgaaccc cgcccgcggc 3361 cacagcgtct gctccacctc cagcttgtac ctgcaggatc tgagcgccgc cgcctcagag 3421 tgcatcgacc cctcggtggt cttcccctac cctctcaacg acagcagctc gcccaagtcc 3481 tgcgcctcgc aagactccag cgccttctct ccgtcctcgg attctctgct ctcctcgacg 3541 gagtcctccc cgcagggcag ccccgagccc ctggtgctcc atgaggagac accgcccacc 3601 accagcagcg actctgagga ggaacaagaa gatgaggaag aaatcgatgt tgtttctgtg 3661 gaaaagaggc aggctcctgg caaaaggtca gagtctggat caccttctgc tggaggccac 3721 agcaaacctc ctcacagccc actggtcctc aagaggtgcc acgtctccac acatcagcac 3781 aactacgcag cgcctccctc cactcggaag gactatcctg ctgccaagag ggtcaagttg 3841 gacagtgtca gagtcctgag acagatcagc aacaaccgaa aatgcaccag ccccaggtcc 3901 tcggacaccg aggagaatgt caagaggcga acacacaacg tcttggagcg ccagaggagg 3961 aacgagctaa aacggagctt ttttgccctg cgtgaccaga tcccggagtt ggaaaacaat 4021 gaaaaggccc ccaaggtagt tatccttaaa aaagccacag catacatcct gtccgtccaa 4081 gcagaggagc aaaagctcat ttctgaagag gacttgttgc ggaaacgacg agaacagttg 4141 aaacacaaac ttgaacagct acggaactct tgtgcgcatc atcaccatca ccattaacta 4201 gttctagagc ggccgcggat cccgcccctc tccctccccc ccccctaacg ttactggccg 4261 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc 4321 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag 4381 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt 4441 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa 4501 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc 4561 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg 4621 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat 4681 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa 4741 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc 4801 ttgccacaac ccacaaggag acgaccttcc atgaccgagt acaagcccac ggtgcgcctc 4861 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac 4921 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa 4981 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 5041 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 5101 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5161 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5221 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5281 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 5341 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5401 tggtgcatga cccgcaagcc cggtgcctag acgcgtctgg aacaatcaac ctctggatta 5461 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 5521 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 5581 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 5641 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 5701 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 5761 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 5821 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg 5881 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 5941 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 6001 gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta 6061 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg 6121 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga 6181 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg 6241 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca 6301 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg 6361 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat 6421 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag 6481 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag 6541 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct 6601 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc 6661 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc 6721 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 6781 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 6841 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 6901 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg 6961 tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 7021 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 7081 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 7141 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 7201 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 7261 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 7321 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 7381 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 7441 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 7501 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 7561 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 7621 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 7681 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 7741 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7801 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 7861 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 7921 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 7981 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 8041 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 8101 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 8161 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 8221 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 8281 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 8341 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 8401 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 8461 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 8521 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 8581 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 8641 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 8701 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 8761 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 8821 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 8881 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 8941 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 9001 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 9061 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 9121 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt 9181 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 9241 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 9301 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc 9361 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat 9421 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttttta 9481 aagaaattgt atttgttaaa tatgtactac aaacttagta gt //