LOCUS       V022913                12698 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022913
VERSION     V022913
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2809..2818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2818..2847
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2854..7374
                     /label="GEMIN5(NM_015465)"
                     /note="GEMIN5(NM_015465)"
                     /gene="GEMIN5"
     misc_feature    7401..7987
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             8007..8606
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8620..9208
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9415..10048
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(10176..10192)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10200..10216
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10224..10254)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10269..10290
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10578..11166)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11337..12197)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12198..12302)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    12350..12484
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggaa
     2821 caaaaactca tctcagaaga ggatctgctc gagatggggc aggagccgcg gacgctgccg
     2881 ccctccccca actggtactg cgcccgctgc agcgatgccg tgcccggggg cctctttggc
     2941 ttcgccgcgc ggacctccgt cttccttgtc cgcgtgggcc cgggcgcagg cgagagtcca
     3001 gggacacccc cgtttcgagt cataggagag ttggtgggac acaccgaaag ggtctctggc
     3061 ttcacatttt ctcatcaccc tggtcagtac aacctctgtg ccaccagctc cgacgatggg
     3121 actgtgaaaa tatgggatgt agagacaaaa acagttgtga cagaacatgc actccatcag
     3181 catacgatat caacattaca ttggtctcct cgagtaaagg acttaatagt atctggggat
     3241 gaaaaaggag tagttttctg ttactggttt aacagaaatg acagccagca cctctttata
     3301 gaacccagga caattttctg tcttacttgt tcacctcatc atgaagattt agtagccatt
     3361 ggctacaagg atggcatagt ggtgataatt gacatcagta agaaaggaga agttattcat
     3421 aggcttcgag gccatgatga tgaaatccac tccatagcct ggtgtcccct gcctggtgaa
     3481 gattgtttat ctataaacca agaggaaact tcagaagctg aaattaccaa cgggaatgct
     3541 gtagcacaag ctccagtaac aaaaggttgc tacttagcca ctggaagcaa agatcaaacc
     3601 attcgaatct ggagctgttc tagaggccga ggggtgatga ttttgaaatt gccctttctg
     3661 aagagaagag gagggggtat agacccaact gttaaagagc gcctttggtt gacactccat
     3721 tggcccagca atcaaccaac acagctggta tctagctgtt ttggaggtga actgttgcaa
     3781 tgggatctca ctcaatcttg gagacggaaa tacaccctct tcagtgcctc atcagaaggg
     3841 caaaatcatt caagaattgt gtttaattta tgtcctttac aaacagagga tgacaaacag
     3901 ctattacttt ctacatcaat ggatagagat gtaaaatgtt gggacatagc caccttggag
     3961 tgcagctgga cccttccttc ccttggtggg tttgcataca gcctggcttt ctcttctgtg
     4021 gacataggct ctttggccat aggtgttggg gatggcatga tccgtgtatg gaatacactc
     4081 tccataaaga acaactatga tgtgaaaaat ttttggcaag gcgtgaagtc caaggttaca
     4141 gcgctgtgct ggcacccaac caaggaaggt tgcttagctt ttggaactga tgatggaaaa
     4201 gtgggattgt atgacaccta ctccaacaag cctccacaga tttctagcac atatcataag
     4261 aagactgtat atactttagc ctgggggcca ccagtacccc ccatgtcact tggaggagaa
     4321 ggagacagac cttcccttgc tttatacagc tgtggaggag aagggattgt cttacagcat
     4381 aatccctgga agcttagtgg agaagccttt gacatcaaca aactcatcag ggacaccaat
     4441 tcaatcaaat acaaattgcc tgtacacaca gagataagtt ggaaagcaga tggcaaaatc
     4501 atggctcttg gcaatgaaga tggatcaata gaaatatttc agattcccaa cctgaaactg
     4561 atctgtacta tccaacagca tcacaagctt gtgaatacca ttagctggca tcatgagcat
     4621 ggcagccagc cagaattgag ctatctgatg gcctctggct ccaacaatgc agtcatttac
     4681 gtgcacaacc tgaagactgt catagagagc agccctgagt ctccagtgac cattacagag
     4741 ccctaccgga ccctctcagg gcatacggcc aagattacca gtgtggcgtg gagcccacat
     4801 catgatggaa ggctggtatc tgcttcctat gatggtacag cccaggtgtg ggatgctctc
     4861 cgggaagagc ccctgtgcaa tttccgagga catcgaggtc gactgctttg tgtggcatgg
     4921 tctcctttgg atccagactg catctattca ggggcagatg acttttgtgt gcacaagtgg
     4981 ctcacttcca tgcaagatca ttcccggcct cctcaaggca aaaaaagtat tgaattggag
     5041 aaaaaacggc tctctcaacc taaggcaaag cccaaaaaga agaaaaagcc caccttgaga
     5101 actcctgtaa agctggaatc gattgatgga aatgaagaag aaagcatgaa ggagaactca
     5161 ggacctgttg agaatggtgt gtcagaccaa gaaggggagg agcaagcacg ggagccggaa
     5221 ttaccctgtg gccttgctcc agcggtttct agagaaccag ttatctgcac tccagtttcc
     5281 tcaggctttg aaaagtcaaa agtcaccatt aataacaaag tcattttact gaaaaaggag
     5341 ccaccaaaag agaagccaga aaccttaatc aagaagagaa aagctcgttc cttgcttccc
     5401 ctgagtacaa gcctggacca cagatccaaa gaggagcttc atcaggactg tttggtacta
     5461 gcaactgcaa agcactccag agagctgaat gaagatgtgt ctgctgatgt tgaggaaaga
     5521 tttcatctgg ggcttttcac agacagggct accctgtata gaatgattga tattgaagga
     5581 aaaggtcact tagaaaatgg ccaccctgag ttatttcacc agcttatgct ttggaaagga
     5641 gatctcaaag gtgttctcca gactgcagca gaaagagggg agctgacaga caaccttgtg
     5701 gctatggcac cagcagctgg ctaccatgtg tggctatggg ctgtggaagc ttttgccaaa
     5761 cagctgtgtt ttcaggatca gtatgtcaag gctgcttctc acctactttc catccacaaa
     5821 gtgtatgaag cggtggagct gctcaagtca aaccattttt acagggaagc tattgcgatt
     5881 gccaaggccc ggctgcgccc ggaggaccca gtcctgaagg acttgtacct cagctgggga
     5941 accgtcctag aaagagatgg ccactatgct gtagctgcca aatgctattt aggggccact
     6001 tgtgcttatg atgcagccaa agttttggcc aaaaaggggg atgcggcatc acttagaacg
     6061 gctgcagagt tggctgccat cgtaggagag gatgagttgt ctgcttccct ggctctcaga
     6121 tgtgcccaag agctgcttct ggccaacaac tgggtgggag cccaggaagc cctgcagctg
     6181 catgaaagtc tacagggtca gagattggtg ttttgccttc tggagctact gtccaggcat
     6241 ctggaggaaa agcagctttc agagggcaaa agctcctcct cttaccacac ttggaacacg
     6301 ggcaccgaag ggcctttcgt ggagagggtg actgcagtgt ggaagagcat cttcagcctt
     6361 gacacccctg agcagtatca ggaagccttt cagaagctgc agaacatcaa gtacccatct
     6421 gctacaaata acacacctgc caaacagctc ctgcttcaca tttgccatga cttgaccctg
     6481 gcagtgctga gccaacagat ggcctcctgg gacgaggctg tgcaggcgct ccttcgggcg
     6541 gtggtccgga gctatgactc agggagcttc accatcatgc aggaagtgta ctcagccttt
     6601 ctccctgacg gctgtgacca cctaagagac aagttggggg accatcaatc ccctgccaca
     6661 ccagctttca aaagtttgga ggcctttttt ctttatgggc gtctgtatga attctggtgg
     6721 tctctctcca gaccttgccc aaattccagt gtctgggtaa gggctggtca cagaacactc
     6781 tctgttgagc caagccagca gttagacact gccagcactg aagaaacgga ccctgaaact
     6841 tctcagccag agccaaacag gccttcagaa ctagacttga gactcacaga agaaggtgag
     6901 cgaatgctga gtacttttaa ggagctcttt tcagaaaagc atgccagtct ccaaaactca
     6961 cagagaactg ttgctgaagt ccaagagacc ttggcagaaa tgatccgaca acaccaaaag
     7021 agtcaactct gtaaatccac agcaaatggt cctgataaga atgaaccgga agtagaagca
     7081 gagcagcccc tctgcagttc tcagagccag tgtaaagaag aaaaaaatga gccactttct
     7141 ctgcctgagt taaccaaaag gcttaccgag gcaaatcaga gaatggcgaa atttcctgag
     7201 agcattaagg cctggccctt cccagatgtg ctggagtgct gcctcgtcct gcttctcatc
     7261 aggtcccact ttcctggctg tctggcccag gaaatgcagc agcaggccca agagctcctt
     7321 cagaaatacg gcaacacgaa aacttacaga agacactgcc agaccttctg tatgtagttc
     7381 tagagcggcc gcggatcccg cccctctccc tccccccccc ctaacgttac tggccgaagc
     7441 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     7501 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     7561 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     7621 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     7681 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     7741 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     7801 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     7861 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     7921 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc
     7981 cacaacccac aaggagacga ccttccatga ccgagtacaa gcccacggtg cgcctcgcca
     8041 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     8101 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     8161 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     8221 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     8281 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     8341 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     8401 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     8461 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     8521 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     8581 gcatgacccg caagcccggt gcctagacgc gtctggaaca atcaacctct ggattacaaa
     8641 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     8701 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     8761 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     8821 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     8881 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     8941 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     9001 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     9061 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     9121 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     9181 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     9241 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     9301 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     9361 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     9421 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     9481 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     9541 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     9601 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     9661 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     9721 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     9781 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     9841 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     9901 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     9961 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10021 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
    10081 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgttta
    10141 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    10201 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    10261 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    10321 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    10381 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10441 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10501 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10561 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10621 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    10681 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10741 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    10801 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    10861 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10921 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10981 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    11041 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    11101 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    11161 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    11221 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    11281 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    11341 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    11401 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    11461 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    11521 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    11581 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    11641 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    11701 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    11761 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    11821 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    11881 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    11941 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    12001 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    12061 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    12121 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    12181 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    12241 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    12301 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat
    12361 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    12421 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    12481 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    12541 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    12601 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga
    12661 aattgtattt gttaaatatg tactacaaac ttagtagt
//
