LOCUS       V033218                13293 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033218
VERSION     V033218
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2749
                     /label="ZBTB39(NM_014830)"
                     /note="ZBTB39(NM_014830)"
                     /gene="ZBTB39"
     CDS             2750..2776
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2822..3163
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3289..3788
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3809..4516
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4517..4573
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4574..5173
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5184..5772
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5835..6068
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6093..6111)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6900..7004
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7005..7865
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8036..8624
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8870..9199
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10333..10398
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10528..10548
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10973..11107
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11276..11909
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11956..12081
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12573..12806
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12991..13035
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctttctac tcggcagtga
      601 actccaagcc accatgggca tgaggatcaa actgcaaagc accaaccacc ccaacaacct
      661 gctgaaggaa ctcaacaagt gccggctctc agagaccatg tgcgacgtca ccattgtggt
      721 ggggagccgc tccttcccgg cccacaaggc tgtgctggcc tgtgcagctg gctacttcca
      781 gaacctcttc ctgaatactg ggcttgatgc tgccaggacc tatgtggtgg acttcatcac
      841 ccctgccaac tttgagaagg ttctgagctt tgtctacact tcagaactct tcacagacct
      901 gatcaatgtt ggggtcatct acgaggtagc tgagcgtctg ggtatggagg acctcctcca
      961 ggcctgtcac tctacctttc ctgatctgga gagcactgcc agggccaagc ccctgaccag
     1021 caccagtgag agccactctg gtaccctgag ttgtccttcg gcagaacctg cccatcccct
     1081 tggagaactc cgaggtggtg gggactacct tggtgctgat agaaactatg tgttgcccag
     1141 tgatgctgga gggagctata aagaggaaga gaagaatgtt gccagtgacg ctaaccatag
     1201 cctgcatctg ccgcaaccgc ccccaccacc gccaaagaca gaagaccatg acacccctgc
     1261 tcccttcacg tccattccta gcatgatgac ccagccactc ctaggcactg tcagcacggg
     1321 catccagacc agcacgagct cctgccagcc atacaaagtt caaagcaatg gagacttcag
     1381 taaaaacagc ttcctcaccc ctgacaatgc agtagacatt accactggga ccaactcctg
     1441 tctgagcaat agtgagcact ccaaagatcc tggctttggg cagatggatg agctccagct
     1501 cgaggacctg ggggatgatg acttgcagtt tgaagaccct gctgaggata taggcacaac
     1561 tgaggaggtg attgagctga gtgatgacag tgaggatgag ttggcttttg gagagaatga
     1621 caatcgggag aataaggcca tgccctgcca ggtgtgcaag aaagttctag agcccaacat
     1681 tcaactgatc cggcagcatg ctcgggacca tgtggacctg ctgacgggca actgcaaggt
     1741 ctgcgagacc cacttccagg accgaaactc ccgggtaact catgtcctgt cccacattgg
     1801 tattttcctt ttctcctgcg acatgtgtga aactaagttc tttacccagt ggcagctgac
     1861 ccttcaccga cgggatggaa tatttgagaa caacatcatt gtccacccca acgatcccct
     1921 gccagggaag ctgggtctct tttcaggggc agcctcccca gagctgaaat gcgctgcctg
     1981 tgggaaagta ttggccaaag atttccatgt ggtccggggc cacatccttg accatctaaa
     2041 cttgaagggc caggcctgca gtgtctgcga ccagcgtcac cttaacctct gcagcctcat
     2101 gtggcacacg ctgtcccatc tcggcatctc agtcttctcc tgttctgtct gtgcgaacag
     2161 ctttgtggac tggcatcttc tagagaagca catggctgtg caccaaagtc tggaagacgc
     2221 cctcttccac tgccgcttgt gcagccagag cttcaagtca gaggctgcct atcgctacca
     2281 cgtcagccag cacaaatgca acagtggcct tgatgcacgg cctggttttg ggctgcagca
     2341 cccagctctc cagaagcgga agctgccagc agaggagttt ctgggtgaag agctggcgct
     2401 gcagggccaa cctgggaaca gcaagtatag ctgcaaggtc tgtggcaaaa gatttgccca
     2461 cacaagcgaa ttcaactacc accggcggat ccacacgggg gagaagccat accaatgtaa
     2521 ggtgtgccac aagttctttc gaggccgctc gaccatcaag tgccacctaa agacacactc
     2581 gggggccctc atgtaccgct gcacagtctg tgggcactac agttccaccc ttaacctcat
     2641 gagcaaacat gttggtgtgc acaaaggcag cctcccccct gacttcacca tcgagcagac
     2701 cttcatgtac atcatccatt ccaaagaggc ggataagaac ccggacagtt acccctacga
     2761 cgtgcctgac tacgccggcg gcggtggttc aggcggtggc ggttccggtg gtgggggatc
     2821 agtccaactg gtggagtctg gtggcgcttt ggtgcagcca ggtggctctc tgcgtttgtc
     2881 ctgtgccgct tctggcttcc cagtgaaccg ctattccatg cgctggtatc gccaggctcc
     2941 aggcaaagag cgtgagtggg tagccggtat gtccagcgcg ggtgatcgta gctcctatga
     3001 agactccgtg aagggccgtt tcaccatcag ccgtgacgat gcccgtaaca cggtgtatct
     3061 gcaaatgaac agcttgaaac ctgaagatac ggccgtgtat tactgtaatg tgaacgtggg
     3121 cttcgagtat tggggccaag gcacccaggt caccgtctcc agctaagatc cttgacttgc
     3181 ggccgcaact cccacctgca acatgcgtga ctgactgagg ccgcgactct agagtcgacc
     3241 tgcatctagg cgccggaatt agatctctcg aggttaacga attctaccgg gtaggggagg
     3301 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     3361 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     3421 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     3481 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     3541 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     3601 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     3661 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     3721 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     3781 gcctttcgac ctgcagccca agcttaccat ggtgagcaag ggcgaggagg ataacatggc
     3841 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     3901 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     3961 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     4021 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     4081 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     4141 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     4201 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     4261 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     4321 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     4381 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     4441 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     4501 ggacgagctg tacaaggcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga
     4561 gaatcctgga ccgatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     4621 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     4681 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     4741 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     4801 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     4861 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     4921 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     4981 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     5041 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     5101 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     5161 gcccggtgcc tgatcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     5221 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     5281 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     5341 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     5401 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     5461 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     5521 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     5581 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     5641 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     5701 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     5761 ccgcctcccc gcatcgatac cgtcgaccaa ggcagctgta gatcttagcc actttttaaa
     5821 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     5881 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5941 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6001 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6061 ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt
     6121 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     6181 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     6241 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     6301 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     6361 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     6421 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     6481 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     6541 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     6601 cataagggag agcgtcgaat ggtgcactct cagtacaatc tagctctgat gccgcatagt
     6661 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     6721 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     6781 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     6841 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     6901 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6961 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7021 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7081 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7141 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7201 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7261 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7321 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7381 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7441 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7501 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7561 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7621 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7681 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7741 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7801 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7861 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7921 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7981 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8041 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8101 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8161 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     8221 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8281 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8341 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8401 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8461 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8521 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8581 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8641 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8701 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8761 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8821 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
     8881 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8941 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9001 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     9061 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9121 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9181 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
     9241 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
     9301 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
     9361 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
     9421 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
     9481 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
     9541 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     9601 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     9661 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     9721 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     9781 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     9841 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     9901 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     9961 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
    10021 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
    10081 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
    10141 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
    10201 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
    10261 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
    10321 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
    10381 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
    10441 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
    10501 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
    10561 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
    10621 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
    10681 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
    10741 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
    10801 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
    10861 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
    10921 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    10981 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    11041 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    11101 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    11161 tattaccact gccaattacc tagtggtttc atttactcta aacctgtgat tcctctgaat
    11221 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa
    11281 gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa
    11341 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
    11401 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
    11461 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
    11521 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
    11581 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
    11641 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
    11701 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
    11761 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    11821 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    11881 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
    11941 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
    12001 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
    12061 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
    12121 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    12181 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
    12241 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    12301 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    12361 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
    12421 gccggtgatc ttcagacctg gacgatatat atgagggaca attggagaag tgaattatat
    12481 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
    12541 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
    12601 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
    12661 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
    12721 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
    12781 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
    12841 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
    12901 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
    12961 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
    13021 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
    13081 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
    13141 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
    13201 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
    13261 tccattcgat tagtgaacgg atctcgacgg tcg
//
