LOCUS       V033303                14361 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033303
VERSION     V033303
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..3817
                     /label="UBE3B(NM_130466)"
                     /note="UBE3B(NM_130466)"
                     /gene="UBE3B"
     CDS             3818..3844
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3890..4231
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        4357..4856
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4877..5584
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5585..5641
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5642..6241
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6252..6840
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6903..7136
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(7161..7179)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7968..8072
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             8073..8933
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      9104..9692
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9938..10267
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          11401..11466
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             11596..11616
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    12041..12175
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             12344..12977
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    13024..13149
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    13641..13874
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             14059..14103
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttagtct gcagcagtga
      601 actccaagcc accatgttca ccctgtctca gacctcgaga gcatggttca tcgatagagc
      661 ccgtcaggca cgagaagaaa ggcttgtgca gaaggaacgg gagcgggcag ctgttgtgat
      721 ccaggcccat gtccggagtt ttctctgtcg gagtcgactg cagagagata tcaggagaga
      781 gattgatgac ttttttaaag cagatgaccc tgagtccact aaaagaagtg cactttgtat
      841 tttcaagatt gccaggaaac tgctgttcct attcagaatc aaagaggata atgagagatt
      901 tgagaagttg tgtcgcagca tcctgagcag catggatgct gagaatgagc ctaaggtgtg
      961 gtatgtgtcc ctggcttgtt ctaaggacct caccctcctt tggattcaac agatcaagaa
     1021 cattttgtgg tactgctgtg attttctcaa gcagctcaag cctgaaatcc tgcaggactc
     1081 ccgactcatc accctgtacc tcacgatgct tgtcaccttc acagacactt caacgtggaa
     1141 aattcttcgg ggaaaaggtg aaagtcttcg accagcgatg aaccacattt gtgcaaatat
     1201 aatgggacat ctcaaccagc atggatttta ttctgtgctg cagatattgt taacccgtgg
     1261 cctggcaaga ccccgtcctt gtctatccaa aggcacttta acagcagctt tttctctagc
     1321 gttacgccct gtgattgctg cacagttctc agacaatctg attcggccgt tcctcatcca
     1381 catcatgtct gtgcctgctc tggtgactca tctcagcaca gtgacccctg agcgcctcac
     1441 tgttttagaa tcccatgaca tgcttcgtaa attcatcata tttttaagag accaagatcg
     1501 atgccgtgat gtatgtgaaa gtttagaagg atgccatacg ctttgtctaa tgggcaacct
     1561 cctacacttg ggctccctca gccccagagt gttagaggag gagacagatg ggttcgtgag
     1621 tttgctcacc cagacgctgt gctactgtcg gaagtatgtg tctcagaaga agtccaacct
     1681 gacccactgg catcctgtcc ttggctggtt ctcccaatct gtggattatg gccttaacga
     1741 gtcaatgcac ttgatcacca aacagctgca gttcttgtgg ggggtgcctc tgatccggat
     1801 cttcttctgt gacatcctga gcaagaagct actggagagc caggagccag cccacgcaca
     1861 gccagcatcc cctcagaatg tgctcccagt gaagagtctc ctaaagcgtg cttttcaaaa
     1921 gtcggcatca gtccggaata ttctcaggcc tgtcgggggt aaacgggtcg actctgcaga
     1981 agtccagaag gtttgcaaca tctgtgtcct ctaccagacc tcgctgacaa ctctcacaca
     2041 gattcggctg cagatactca caggtctcac ttaccttgat gacctgcttc ccaaactgtg
     2101 ggcatttatc tgtgagctcg ggccccacgg agggttaaag ctcttcttgg aatgcctgaa
     2161 caatgacact gaagagtcca agcaactctt ggccatgctg atgctgttct gtgactgttc
     2221 gcggcacctc atcacaatcc ttgatgacat tgaagtttat gaagaacaga tttcattcaa
     2281 actggaagag ctggtcacta tctcctcttt cctgaattct tttgtgttta agatgatctg
     2341 ggatggaatt gtagagaacg ccaagggtga gaccttggag ctgttccagt ctgtccacgg
     2401 gtggcttatg gtgctgtacg agcgggactg ccggcggcgc ttcacccccg aggaccactg
     2461 gctgcgaaag gatctcaaac ctagcgtgct cttccaagaa ctcgacaggg acagaaaacg
     2521 ggcacagttg atcctgcagt acatcccaca tgtcatccct cacaaaaaca gagttctact
     2581 gtttcgaacc atggttacca aggagaagga gaaactgggg ctggtggaaa ccagctctgc
     2641 ctccccgcat gtcactcaca tcaccatccg ccggtccagg atgctggagg acggctacga
     2701 gcagcttagg cagctctccc agcacgccat gaagggggtc atccgtgtga agtttgtcaa
     2761 tgacctcggg gtggacgaag cagggattga tcaagacggt gtttttaagg agttcttgga
     2821 agagatcatc aagagagttt ttgacccagc actcaatctg ttcaagacaa ccagtgggga
     2881 tgagaggctg tacccctcac ccacatccta catccatgag aattacctgc agctcttcga
     2941 gtttgtgggg aagatgctgg ggaaggctgt gtatgaggga attgtggtgg acgtgccatt
     3001 tgcatccttc ttcctgagcc aactgcttgg gcaccaccac agcgtcttct atagctcggt
     3061 ggatgaactg ccttctctgg actccgagtt ctataaaaac ctcacctcca tcaagcgcta
     3121 tgatggggac atcactgacc tgggcctgac gctgtcttac gacgaggacg tcatgggtca
     3181 gcttgtttgc catgaactga ttcctggagg gaagaccatt cctgttacaa atgaaaataa
     3241 aattagctac atccatctga tggcacattt tcgaatgcac actcaaataa aaaaccaaac
     3301 agctgccctc attagcggat tccgttccat tatcaaaccc gagtggatcc gaatgttctc
     3361 aactcctgaa ctgcagcgtc tcatctctgg cgacaatgct gagattgatc tggaagattt
     3421 aaagaagcac acagtctact acggtggttt ccatggaagt cacagagtca tcatctggct
     3481 ctgggatatt ctggcctccg acttcacacc ggatgagaga gctatgtttc tgaagttcgt
     3541 gaccagctgc tccagacccc cgctcctggg attcgcctac ctcaagcctc cattctccat
     3601 ccgctgcgtg gaggtgtcgg acgatcagga caccggggac actctgggca gcgtcctccg
     3661 gggcttcttc accatccgca agcgggagcc aggcggccgc ctgcccacct cctccacctg
     3721 cttcaacctg ctcaagctgc ccaactacag caagaagagc gtcctccgcg agaagctgcg
     3781 ctacgccatc agcatgaaca cgggctttga actctcctac ccctacgacg tgcctgacta
     3841 cgccggcggc ggtggttcag gcggtggcgg ttccggtggt gggggatcag tccaactggt
     3901 ggagtctggt ggcgctttgg tgcagccagg tggctctctg cgtttgtcct gtgccgcttc
     3961 tggcttccca gtgaaccgct attccatgcg ctggtatcgc caggctccag gcaaagagcg
     4021 tgagtgggta gccggtatgt ccagcgcggg tgatcgtagc tcctatgaag actccgtgaa
     4081 gggccgtttc accatcagcc gtgacgatgc ccgtaacacg gtgtatctgc aaatgaacag
     4141 cttgaaacct gaagatacgg ccgtgtatta ctgtaatgtg aacgtgggct tcgagtattg
     4201 gggccaaggc acccaggtca ccgtctccag ctaagatcct tgacttgcgg ccgcaactcc
     4261 cacctgcaac atgcgtgact gactgaggcc gcgactctag agtcgacctg catctaggcg
     4321 ccggaattag atctctcgag gttaacgaat tctaccgggt aggggaggcg cttttcccaa
     4381 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     4441 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     4501 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     4561 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     4621 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     4681 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     4741 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     4801 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     4861 gcagcccaag cttaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     4921 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     4981 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     5041 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     5101 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     5161 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     5221 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     5281 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     5341 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     5401 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     5461 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     5521 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     5581 caaggcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc
     5641 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     5701 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     5761 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     5821 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     5881 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     5941 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6001 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6061 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6121 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6181 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6241 atcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6301 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6361 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6421 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6481 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6541 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6601 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6661 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6721 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6781 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6841 atcgataccg tcgaccaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6901 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6961 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7021 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7081 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     7141 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     7201 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     7261 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     7321 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     7381 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     7441 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     7501 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     7561 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     7621 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     7681 cgtcgaatgg tgcactctca gtacaatcta gctctgatgc cgcatagtta agccagcccc
     7741 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     7801 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     7861 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     7921 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7981 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8041 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     8101 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8161 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8221 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8281 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     8341 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8401 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     8461 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     8521 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     8581 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8641 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8701 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8761 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8821 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8881 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8941 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9001 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9061 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9121 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9181 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9241 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9301 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9361 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9421 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9481 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9541 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9601 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9661 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9721 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9781 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9841 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     9901 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     9961 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    10021 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    10081 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    10141 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    10201 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
    10261 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
    10321 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
    10381 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
    10441 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
    10501 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
    10561 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
    10621 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
    10681 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
    10741 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
    10801 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
    10861 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
    10921 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
    10981 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
    11041 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
    11101 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
    11161 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
    11221 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
    11281 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
    11341 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
    11401 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
    11461 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
    11521 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
    11581 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
    11641 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
    11701 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
    11761 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
    11821 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
    11881 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
    11941 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    12001 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    12061 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    12121 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    12181 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    12241 caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    12301 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
    12361 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    12421 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
    12481 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
    12541 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
    12601 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
    12661 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
    12721 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
    12781 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    12841 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    12901 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    12961 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
    13021 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
    13081 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
    13141 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
    13201 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
    13261 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
    13321 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
    13381 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    13441 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggtgatctt
    13501 cagacctgga cgatatatat gagggacaat tggagaagtg aattatataa atataaagta
    13561 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
    13621 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
    13681 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
    13741 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
    13801 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
    13861 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
    13921 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
    13981 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    14041 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    14101 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    14161 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    14221 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
    14281 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
    14341 gtgaacggat ctcgacggtc g
//
