LOCUS       V034029                12513 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034029
VERSION     V034029
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1969
                     /label="TRIM49B(NM_001206626)"
                     /note="TRIM49B(NM_001206626)"
                     /gene="TRIM49B"
     CDS             1970..1996
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2042..2383
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2509..3008
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3029..3736
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3737..3793
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3794..4393
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4404..4992
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5055..5288
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5313..5331)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6120..6224
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6225..7085
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7256..7844
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8090..8419
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9553..9618
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9748..9768
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10193..10327
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10496..11129
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11176..11301
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11793..12026
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12211..12255
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctggtacc gcagcagtga
      601 actccaagcc accatgaatt ctggaatctt acaggtcttt cagagggaac tcatctgccc
      661 catctgcatg aactacttca tagacccggt caccatagac tgtgggcaca gcttttgcag
      721 gccttgtttc tacctcaact ggaaagacag cccatttctt gtccagtgct ctgaatgcac
      781 aaagtcaaca gggcagataa acctcaaaac caacattcat ttcaagaaga tggcttctct
      841 tgctagaaaa gtcagtctct ggctattcct gagctctgag gagcaaatgt gtggcactca
      901 cagggagaca aagaagatgt tctgtgaagt ggacaggagc ctgctctgtt tgctgtgctc
      961 cagctctcag gagcaccggg atcacagaca ctgtcccatt gagtcggctg ctgaggaaca
     1021 ccaggagaag cttttacaga aaatgcagtc tttgtgggaa aaagcttgtg aaaatcacag
     1081 aaacctgaat gtggaaacca ccagaaccag atgctggaag gattatgtga atttaaggct
     1141 agaagcaatt agagctgagt atcagaagat gcctgcattt caccatgaag aagaaaaaca
     1201 taatttggag atgctgaaaa agaaggggaa agatattttt catcgacttc atttaagtaa
     1261 agccaaaatg gctcatagga gggagatttt aagaggaatg tatgaggagc tgaacgaaat
     1321 gtgccataaa ccagatgtgg agctacttca ggcttttgga gacatattac acaggagtga
     1381 gtccgtgctg ctgcacatgc cccagcctct gaatccagag ctcagtgcag ggcccatcac
     1441 tggactgagg gacaggctca accaattccg agtgcatatt actctgcatc atgaagaagc
     1501 caacagtgat atctttctat gtgaaatttt gagaagcatg tgtattggat gtgaccatca
     1561 agatgtaccc tatttcactg caacacctag aagttttctt gcatggggtg ctcagacttt
     1621 cacctcgggc aaatattact gggaggtcca tgtaggggac tcctggaatt gggcttttgg
     1681 tgtctgtaat atgtattgga aagagaagaa tcagaatgag aagatagatg gagaggatgg
     1741 actctttctt cttgggtgtg ttaagaatga cattcaacgc agtctcttta ccacctcccc
     1801 acttctgctg caatatatcc caagacctac cagccgagta ggattattcc tggattgtga
     1861 ggctaagact gtgagctttg ttgatgttaa tcaaagctcc ctaatataca ccatccctaa
     1921 ttgctctttc tcacctcctc tcaggcctat cttttgctgt attcacttct acccctacga
     1981 cgtgcctgac tacgccggcg gcggtggttc aggcggtggc ggttccggtg gtgggggatc
     2041 agtccaactg gtggagtctg gtggcgcttt ggtgcagcca ggtggctctc tgcgtttgtc
     2101 ctgtgccgct tctggcttcc cagtgaaccg ctattccatg cgctggtatc gccaggctcc
     2161 aggcaaagag cgtgagtggg tagccggtat gtccagcgcg ggtgatcgta gctcctatga
     2221 agactccgtg aagggccgtt tcaccatcag ccgtgacgat gcccgtaaca cggtgtatct
     2281 gcaaatgaac agcttgaaac ctgaagatac ggccgtgtat tactgtaatg tgaacgtggg
     2341 cttcgagtat tggggccaag gcacccaggt caccgtctcc agctaagatc cttgacttgc
     2401 ggccgcaact cccacctgca acatgcgtga ctgactgagg ccgcgactct agagtcgacc
     2461 tgcatctagg cgccggaatt agatctctcg aggttaacga attctaccgg gtaggggagg
     2521 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     2581 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     2641 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     2701 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     2761 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     2821 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     2881 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     2941 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     3001 gcctttcgac ctgcagccca agcttaccat ggtgagcaag ggcgaggagg ataacatggc
     3061 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     3121 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     3181 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     3241 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     3301 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     3361 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     3421 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     3481 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     3541 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     3601 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     3661 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     3721 ggacgagctg tacaaggcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga
     3781 gaatcctgga ccgatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     3841 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     3901 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     3961 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     4021 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     4081 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     4141 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     4201 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     4261 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     4321 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     4381 gcccggtgcc tgatcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     4441 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4501 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4561 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4621 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4681 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4741 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     4801 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4861 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4921 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4981 ccgcctcccc gcatcgatac cgtcgaccaa ggcagctgta gatcttagcc actttttaaa
     5041 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     5101 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5161 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5221 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5281 ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt
     5341 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     5401 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     5461 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     5521 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     5581 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     5641 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     5701 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     5761 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     5821 cataagggag agcgtcgaat ggtgcactct cagtacaatc tagctctgat gccgcatagt
     5881 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5941 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     6001 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     6061 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     6121 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6181 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6241 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6301 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6361 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6421 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6481 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6541 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6601 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6661 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6721 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6781 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6841 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6901 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6961 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7021 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7081 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7141 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7201 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7261 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7321 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7381 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     7441 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7501 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7561 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7621 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7681 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7741 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7801 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7861 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7921 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7981 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8041 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
     8101 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8161 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8221 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     8281 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8341 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8401 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
     8461 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
     8521 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
     8581 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
     8641 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
     8701 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
     8761 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     8821 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     8881 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     8941 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     9001 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     9061 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     9121 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     9181 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     9241 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     9301 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
     9361 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     9421 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
     9481 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
     9541 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
     9601 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
     9661 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
     9721 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
     9781 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
     9841 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
     9901 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
     9961 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
    10021 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
    10081 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
    10141 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    10201 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10261 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10321 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    10381 tattaccact gccaattacc tagtggtttc atttactcta aacctgtgat tcctctgaat
    10441 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa
    10501 gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa
    10561 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
    10621 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
    10681 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
    10741 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
    10801 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
    10861 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
    10921 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
    10981 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    11041 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    11101 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
    11161 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
    11221 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
    11281 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
    11341 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    11401 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
    11461 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    11521 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    11581 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
    11641 gccggtgatc ttcagacctg gacgatatat atgagggaca attggagaag tgaattatat
    11701 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
    11761 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
    11821 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
    11881 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
    11941 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
    12001 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
    12061 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
    12121 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
    12181 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
    12241 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
    12301 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
    12361 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
    12421 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
    12481 tccattcgat tagtgaacgg atctcgacgg tcg
//
