LOCUS       V030086                13527 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030086
VERSION     V030086
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2983
                     /label="TNFAIP3(NM_001270508)"
                     /note="TNFAIP3(NM_001270508)"
                     /gene="TNFAIP3"
     CDS             2984..3010
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3056..3397
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3523..4022
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4043..4750
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4751..4807
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4808..5407
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5418..6006
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6069..6302
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6327..6345)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7134..7238
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7239..8099
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8270..8858
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        9104..9433
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10567..10632
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10762..10782
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11207..11341
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11510..12143
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12190..12315
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12807..13040
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13225..13269
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctgacgtt attgcagtga
      601 actccaagcc accatggctg aacaagtcct tcctcaggct ttgtatttga gcaatatgcg
      661 gaaagctgtg aagatacggg agagaactcc agaagacatt tttaaaccta ctaatgggat
      721 cattcatcat tttaaaacca tgcaccgata cacactggaa atgttcagaa cttgccagtt
      781 ttgtcctcag tttcgggaga tcatccacaa agccctcatc gacagaaaca tccaggccac
      841 cctggaaagc cagaagaaac tcaactggtg tcgagaagtc cggaagcttg tggcgctgaa
      901 aacgaacggt gacggcaatt gcctcatgca tgccacttct cagtacatgt ggggcgttca
      961 ggacacagac ttggtactga ggaaggcgct gttcagcacg ctcaaggaaa cagacacacg
     1021 caactttaaa ttccgctggc aactggagtc tctcaaatct caggaatttg ttgaaacggg
     1081 gctttgctat gatactcgga actggaatga tgaatgggac aatcttatca aaatggcttc
     1141 cacagacaca cccatggccc gaagtggact tcagtacaac tcactggaag aaatacacat
     1201 atttgtcctt tgcaacatcc tcagaaggcc aatcattgtc atttcagaca aaatgctaag
     1261 aagtttggaa tcaggttcca atttcgcccc tttgaaagtg ggtggaattt acttgcctct
     1321 ccactggcct gcccaggaat gctacagata ccccattgtt ctcggctatg acagccatca
     1381 ttttgtaccc ttggtgaccc tgaaggacag tgggcctgaa atccgagctg ttccacttgt
     1441 taacagagac cggggaagat ttgaagactt aaaagttcac tttttgacag atcctgaaaa
     1501 tgagatgaag gagaagctct taaaagagta cttaatggtg atagaaatcc ccgtccaagg
     1561 ctgggaccat ggcacaactc atctcatcaa tgccgcaaag ttggatgaag ctaacttacc
     1621 aaaagaaatc aatctggtag atgattactt tgaacttgtt cagcatgagt acaagaaatg
     1681 gcaggaaaac agcgagcagg ggaggagaga ggggcacgcc cagaatccca tggaaccttc
     1741 cgtgccccag ctttctctca tggatgtaaa atgtgaaacg cccaactgcc ccttcttcat
     1801 gtctgtgaac acccagcctt tatgccatga gtgctcagag aggcggcaaa agaatcaaaa
     1861 caaactccca aagctgaact ccaagccggg ccctgagggg ctccctggca tggcgctcgg
     1921 ggcctctcgg ggagaagcct atgagccctt ggcgtggaac cctgaggagt ccactggggg
     1981 gcctcattcg gccccaccga cagcacccag cccttttctg ttcagtgaga ccactgccat
     2041 gaagtgcagg agccccggct gccccttcac actgaatgtg cagcacaacg gattttgtga
     2101 acgttgccac aacgcccggc aacttcacgc cagccacgcc ccagaccaca caaggcactt
     2161 ggatcccggg aagtgccaag cctgcctcca ggatgttacc aggacattta atgggatctg
     2221 cagtacttgc ttcaaaagga ctacagcaga ggcctcctcc agcctcagca ccagcctccc
     2281 tccttcctgt caccagcgtt ccaagtcaga tccctcgcgg ctcgtccgga gcccctcccc
     2341 gcattcttgc cacagagctg gaaacgacgc ccctgctggc tgcctgtctc aagctgcacg
     2401 gactcctggg gacaggacgg ggacgagcaa gtgcagaaaa gccggctgcg tgtattttgg
     2461 gactccagaa aacaagggct tttgcacact gtgtttcatc gagtacagag aaaacaaaca
     2521 ttttgctgct gcctcaggga aagtcagtcc cacagcgtcc aggttccaga acaccattcc
     2581 gtgcctgggg agggaatgcg gcacccttgg aagcaccatg tttgaaggat actgccagaa
     2641 gtgtttcatt gaagctcaga atcagagatt tcatgaggcc aaaaggacag aagagcaact
     2701 gagatcgagc cagcgcagag atgtgcctcg aaccacacaa agcacctcaa ggcccaagtg
     2761 cgcccgggcc tcctgcaaga acatcctggc ctgccgcagc gaggagctct gcatggagtg
     2821 tcagcatccc aaccagagga tgggccctgg ggcccaccgg ggtgagcctg cccccgaaga
     2881 cccccccaag cagcgttgcc gggcccccgc ctgtgatcat tttggcaatg ccaagtgcaa
     2941 cggctactgc aacgaatgct ttcagttcaa gcagatgtat ggctacccct acgacgtgcc
     3001 tgactacgcc ggcggcggtg gttcaggcgg tggcggttcc ggtggtgggg gatcagtcca
     3061 actggtggag tctggtggcg ctttggtgca gccaggtggc tctctgcgtt tgtcctgtgc
     3121 cgcttctggc ttcccagtga accgctattc catgcgctgg tatcgccagg ctccaggcaa
     3181 agagcgtgag tgggtagccg gtatgtccag cgcgggtgat cgtagctcct atgaagactc
     3241 cgtgaagggc cgtttcacca tcagccgtga cgatgcccgt aacacggtgt atctgcaaat
     3301 gaacagcttg aaacctgaag atacggccgt gtattactgt aatgtgaacg tgggcttcga
     3361 gtattggggc caaggcaccc aggtcaccgt ctccagctaa gatccttgac ttgcggccgc
     3421 aactcccacc tgcaacatgc gtgactgact gaggccgcga ctctagagtc gacctgcatc
     3481 taggcgccgg aattagatct ctcgaggtta acgaattcta ccgggtaggg gaggcgcttt
     3541 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     3601 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     3661 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     3721 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     3781 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     3841 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     3901 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     3961 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     4021 cgacctgcag cccaagctta ccatggtgag caagggcgag gaggataaca tggccatcat
     4081 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     4141 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     4201 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     4261 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     4321 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     4381 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     4441 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     4501 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     4561 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     4621 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     4681 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     4741 gctgtacaag gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc
     4801 tggaccgatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     4861 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     4921 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     4981 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     5041 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5101 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5161 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     5221 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     5281 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     5341 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     5401 tgcctgatct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5461 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5521 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5581 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5641 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5701 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5761 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     5821 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5881 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5941 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6001 ccccgcatcg ataccgtcga ccaaggcagc tgtagatctt agccactttt taaaagaaaa
     6061 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     6121 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6181 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6241 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6301 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
     6361 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     6421 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     6481 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     6541 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     6601 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     6661 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     6721 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     6781 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     6841 ggagagcgtc gaatggtgca ctctcagtac aatctagctc tgatgccgca tagttaagcc
     6901 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     6961 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     7021 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     7081 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     7141 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7201 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7261 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7321 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7381 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7441 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7501 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7561 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7621 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7681 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7741 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7801 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7861 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7921 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7981 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8041 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8101 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8161 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8221 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8281 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8341 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8401 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8461 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8521 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8581 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8641 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8701 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8761 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8821 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8881 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8941 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9001 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9061 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     9121 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9181 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9241 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     9301 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     9361 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     9421 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
     9481 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
     9541 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
     9601 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
     9661 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
     9721 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
     9781 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
     9841 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
     9901 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
     9961 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
    10021 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
    10081 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
    10141 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
    10201 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
    10261 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
    10321 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
    10381 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
    10441 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
    10501 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
    10561 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
    10621 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
    10681 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
    10741 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
    10801 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
    10861 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
    10921 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
    10981 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
    11041 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
    11101 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
    11161 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    11221 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    11281 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    11341 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    11401 cactgccaat tacctagtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    11461 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
    11521 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
    11581 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
    11641 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
    11701 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
    11761 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
    11821 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
    11881 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
    11941 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
    12001 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    12061 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    12121 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
    12181 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
    12241 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
    12301 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
    12361 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
    12421 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
    12481 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
    12541 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
    12601 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggt
    12661 gatcttcaga cctggacgat atatatgagg gacaattgga gaagtgaatt atataaatat
    12721 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    12781 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
    12841 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
    12901 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
    12961 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
    13021 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
    13081 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    13141 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    13201 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    13261 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
    13321 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
    13381 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
    13441 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
    13501 cgattagtga acggatctcg acggtcg
//
