LOCUS       V033290                12105 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033290
VERSION     V033290
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1561
                     /label="TNFAIP1(NM_021137)"
                     /note="TNFAIP1(NM_021137)"
                     /gene="TNFAIP1"
     CDS             1562..1588
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1634..1975
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2101..2600
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             2621..3328
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3329..3385
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3386..3985
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    3996..4584
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             4647..4880
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(4905..4923)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        5712..5816
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5817..6677
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6848..7436
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        7682..8011
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9145..9210
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9340..9360
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    9785..9919
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10088..10721
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    10768..10893
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11385..11618
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             11803..11847
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttgtagc attgcagtga
      601 actccaagcc accatgtcgg gggacacctg cctgtgccca gcctcagggg ccaagcccaa
      661 gctcagtggc ttcaagggag gagggttggg caacaagtat gtccagctca acgtgggcgg
      721 ctctctgtac tacaccactg tgcgggccct gacccgccac gacaccatgc tcaaggccat
      781 gttcagtggg cgcatggagg tgctgaccga caaagaaggc tggatcctca tagaccgttg
      841 tggaaagcac tttggcacca ttttgaatta cctccgagat gacaccatca ccctccctca
      901 gaaccggcaa gaaatcaagg aattgatggc tgaagcaaag tattacctca tccaggggct
      961 ggtgaatatg tgccagagtg ccctgcagga caagaaggac tcctaccagc ctgtgtgcaa
     1021 catccccatc atcacatccc taaaggagga ggagcggctc atcgaatcct ccaccaagcc
     1081 cgtggtgaag ctgctgtaca acagaagcaa caacaagtat tcctacacca gcaactctga
     1141 cgaccacctg ctgaaaaaca tcgagctgtt tgacaagctc tccctgcgct tcaacggccg
     1201 cgtgctcttc atcaaggatg tcattggtga cgagatctgc tgctggtcct tttatggcca
     1261 gggccgtaag ctggcagagg tgtgctgtac ctccatcgtg tatgccacgg agaagaagca
     1321 gaccaaggtg gaattcccag aggcccgaat ctatgaggag acactcaacg tcctactcta
     1381 tgagactccc cgcgtccccg acaactcctt gttggaggcc acaagccgta gccgcagcca
     1441 ggcttccccc agtgaagatg aggagacctt tgaactgcgg gaccgtgtcc gccgcatcca
     1501 cgtcaagcgc tacagcactt acgatgaccg gcagctcggc caccagtcta cccatcgcga
     1561 ctacccctac gacgtgcctg actacgccgg cggcggtggt tcaggcggtg gcggttccgg
     1621 tggtggggga tcagtccaac tggtggagtc tggtggcgct ttggtgcagc caggtggctc
     1681 tctgcgtttg tcctgtgccg cttctggctt cccagtgaac cgctattcca tgcgctggta
     1741 tcgccaggct ccaggcaaag agcgtgagtg ggtagccggt atgtccagcg cgggtgatcg
     1801 tagctcctat gaagactccg tgaagggccg tttcaccatc agccgtgacg atgcccgtaa
     1861 cacggtgtat ctgcaaatga acagcttgaa acctgaagat acggccgtgt attactgtaa
     1921 tgtgaacgtg ggcttcgagt attggggcca aggcacccag gtcaccgtct ccagctaaga
     1981 tccttgactt gcggccgcaa ctcccacctg caacatgcgt gactgactga ggccgcgact
     2041 ctagagtcga cctgcatcta ggcgccggaa ttagatctct cgaggttaac gaattctacc
     2101 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     2161 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     2221 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     2281 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     2341 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     2401 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     2461 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     2521 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     2581 cctcatctcc gggcctttcg acctgcagcc caagcttacc atggtgagca agggcgagga
     2641 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     2701 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     2761 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     2821 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     2881 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     2941 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     3001 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     3061 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     3121 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     3181 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     3241 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     3301 caccggcggc atggacgagc tgtacaaggc aacaaacttc tctctgctga aacaagccgg
     3361 agatgtcgaa gagaatcctg gaccgatgac cgagtacaag cccacggtgc gcctcgccac
     3421 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     3481 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     3541 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     3601 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     3661 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     3721 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     3781 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     3841 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     3901 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     3961 catgacccgc aagcccggtg cctgatctta tcgataatca acctctggat tacaaaattt
     4021 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4081 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4141 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4201 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4261 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4321 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4381 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4441 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4501 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4561 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc aaggcagctg tagatcttag
     4621 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     4681 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     4741 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     4801 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     4861 agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta
     4921 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat
     4981 atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg
     5041 taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc
     5101 ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac
     5161 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     5221 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     5281 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     5341 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     5401 atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctagctctg
     5461 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5521 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5581 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5641 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5701 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5761 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5821 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5881 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5941 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6001 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6061 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6121 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6181 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6241 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6301 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6361 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6421 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6481 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6541 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6601 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6661 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6721 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6781 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6841 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6901 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6961 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     7021 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7081 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7141 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7201 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7261 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7321 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7381 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7441 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7501 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7561 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7621 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
     7681 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7741 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7801 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7861 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7921 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7981 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga
     8041 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
     8101 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
     8161 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
     8221 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
     8281 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc
     8341 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
     8401 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
     8461 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
     8521 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
     8581 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt
     8641 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
     8701 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
     8761 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
     8821 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
     8881 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
     8941 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
     9001 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
     9061 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
     9121 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
     9181 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
     9241 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
     9301 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
     9361 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
     9421 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
     9481 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
     9541 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
     9601 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
     9661 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     9721 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     9781 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9841 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9901 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     9961 caccccatac cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg
    10021 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
    10081 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
    10141 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
    10201 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
    10261 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
    10321 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
    10381 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
    10441 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
    10501 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
    10561 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    10621 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    10681 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    10741 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    10801 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    10861 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    10921 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    10981 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    11041 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    11101 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    11161 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    11221 gcacagcaag cggccggtga tcttcagacc tggacgatat atatgaggga caattggaga
    11281 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    11341 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    11401 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
    11461 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
    11521 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
    11581 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
    11641 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
    11701 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
    11761 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
    11821 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
    11881 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
    11941 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
    12001 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
    12061 gacagagaca gatccattcg attagtgaac ggatctcgac ggtcg
//
