LOCUS       V033301                12243 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033301
VERSION     V033301
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1699
                     /label="TMEM129(NM_001127266)"
                     /note="TMEM129(NM_001127266)"
                     /gene="TMEM129"
     CDS             1700..1726
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1772..2113
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2239..2738
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             2759..3466
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3467..3523
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3524..4123
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4134..4722
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             4785..5018
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5043..5061)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        5850..5954
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5955..6815
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6986..7574
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        7820..8149
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9283..9348
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9478..9498
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    9923..10057
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10226..10859
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    10906..11031
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11523..11756
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             11941..11985
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctaccact aaggcagtga
      601 actccaagcc accatggaca gccccgaggt gaccttcact ctcgcctatc tggtgttcgc
      661 cgtgtgcttc gtgttcacgc ccaacgagtt ccacgcggcg gggctcacgg tgcagaacct
      721 gctgtcgggc tggctgggca gcgaggacgc cgccttcgtg cccttccact tgcgccgcac
      781 ggccgccacg ctgttgtgcc actcgctgct gccgctcggc tactatgtgg gcatgtgcct
      841 tgcggcttca gaaaagcggc tccacgccct cagccaggcc cctgaggcct ggcggctctt
      901 cctgctgctg gccgtgaccc tcccctccat cgcctgcatc ctgatctact actggtcccg
      961 tgaccggtgg gcctgccacc cactggcgcg caccctggcc ctctacgccc tcccacagtc
     1021 tggctggcag gctgttgcct cctctgtcaa cactgagttc cggcggattg acaagtttgc
     1081 caccggtgca ccaggtgccc gtgtgattgt gacagacacg tgggtgatga aggtaaccac
     1141 ctaccgagtg cacgtggccc agcagcagga cgtgcacctg actgtgacgg agtctcggca
     1201 gcatgagctc tcgccagact cgaacttgcc cgtgcagctc ctcaccatcc gtgtggccag
     1261 caccaaccct gctgtgcagg cctttgacat ctggctgaac tccactgagt acggggagct
     1321 ctgcgagaag ctccgggcac ccatccgcag ggcagcccat gtggtcatcc accagagcct
     1381 gggcgacctg ttcctggaga catttgcctc cctggtagag gtcaacccgg cctactcagt
     1441 gcccagcagc caggagctgg aggcctgcat aggctgcatg cagacacgtg ccagcgtgaa
     1501 gctggtgaag acctgccagg aggcagccac aggcgagtgc cagcagtgtt actgccgccc
     1561 catgtggtgc ctcacctgca tgggcaagtg gttcgccagc cgccaggacc ccctgcgccc
     1621 tgacacctgg ctggccagcc gcgtgccctg ccccacctgc cgcgcacgct tctgcatcct
     1681 ggatgtgtgc accgtgcgct acccctacga cgtgcctgac tacgccggcg gcggtggttc
     1741 aggcggtggc ggttccggtg gtgggggatc agtccaactg gtggagtctg gtggcgcttt
     1801 ggtgcagcca ggtggctctc tgcgtttgtc ctgtgccgct tctggcttcc cagtgaaccg
     1861 ctattccatg cgctggtatc gccaggctcc aggcaaagag cgtgagtggg tagccggtat
     1921 gtccagcgcg ggtgatcgta gctcctatga agactccgtg aagggccgtt tcaccatcag
     1981 ccgtgacgat gcccgtaaca cggtgtatct gcaaatgaac agcttgaaac ctgaagatac
     2041 ggccgtgtat tactgtaatg tgaacgtggg cttcgagtat tggggccaag gcacccaggt
     2101 caccgtctcc agctaagatc cttgacttgc ggccgcaact cccacctgca acatgcgtga
     2161 ctgactgagg ccgcgactct agagtcgacc tgcatctagg cgccggaatt agatctctcg
     2221 aggttaacga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     2281 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     2341 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     2401 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     2461 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     2521 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     2581 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     2641 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     2701 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     2761 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     2821 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     2881 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     2941 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     3001 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     3061 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     3121 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     3181 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     3241 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     3301 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     3361 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     3421 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggcaa caaacttctc
     3481 tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccgatgaccg agtacaagcc
     3541 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     3601 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     3661 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     3721 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     3781 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     3841 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     3901 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     3961 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     4021 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     4081 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgatcttatc gataatcaac
     4141 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     4201 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     4261 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     4321 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     4381 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     4441 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4501 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     4561 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4621 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4681 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgaccaa
     4741 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4801 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     4861 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     4921 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4981 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     5041 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     5101 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     5161 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     5221 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     5281 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     5341 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     5401 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     5461 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     5521 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     5581 cagtacaatc tagctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     5641 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     5701 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     5761 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     5821 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5881 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5941 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6001 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6061 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6121 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6181 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6241 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6301 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6361 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6421 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6481 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6541 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6601 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6661 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6721 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6781 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6841 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6901 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6961 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7021 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7081 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     7141 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7201 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7261 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7321 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7381 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7441 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7501 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7561 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7621 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7681 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7741 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7801 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7861 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7921 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7981 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8041 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     8101 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
     8161 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
     8221 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
     8281 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
     8341 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
     8401 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
     8461 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
     8521 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
     8581 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
     8641 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
     8701 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
     8761 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
     8821 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
     8881 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
     8941 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
     9001 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
     9061 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
     9121 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
     9181 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
     9241 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     9301 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     9361 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     9421 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     9481 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     9541 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     9601 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
     9661 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
     9721 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
     9781 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     9841 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     9901 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     9961 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10021 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    10081 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tagtggtttc
    10141 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    10201 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
    10261 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
    10321 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    10381 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
    10441 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
    10501 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
    10561 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
    10621 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
    10681 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10741 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10801 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    10861 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    10921 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    10981 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    11041 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    11101 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    11161 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    11221 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    11281 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    11341 aacaaaagta agaccaccgc acagcaagcg gccggtgatc ttcagacctg gacgatatat
    11401 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    11461 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    11521 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    11581 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    11641 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    11701 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    11761 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    11821 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    11881 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    11941 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    12001 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    12061 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    12121 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    12181 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
    12241 tcg
//
