LOCUS       V030104                13401 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030104
VERSION     V030104
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2857
                     /label="SMURF2(NM_022739)"
                     /note="SMURF2(NM_022739)"
                     /gene="SMURF2"
     CDS             2858..2884
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2930..3271
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3397..3896
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3917..4624
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4625..4681
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4682..5281
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5292..5880
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5943..6176
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6201..6219)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        7008..7112
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7113..7973
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8144..8732
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8978..9307
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10441..10506
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10636..10656
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11081..11215
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11384..12017
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12064..12189
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12681..12914
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13099..13143
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctcgtttt agtgcagtga
      601 actccaagcc accatgtcta accccggagg ccggaggaac gggcccgtca agctgcgcct
      661 gacagtactc tgtgcaaaaa acctggtgaa aaaggatttt ttccgacttc ctgatccatt
      721 tgctaaggtg gtggttgatg gatctgggca atgccattct acagatactg tgaagaatac
      781 gcttgatcca aagtggaatc agcattatga cctgtatatt ggaaagtctg attcagttac
      841 gatcagtgta tggaatcaca agaagatcca taagaaacaa ggtgctggat ttctcggttg
      901 tgttcgtctt ctttccaatg ccatcaaccg cctcaaagac actggttatc agaggttgga
      961 tttatgcaaa ctcgggccaa atgacaatga tacagttaga ggacagatag tagtaagtct
     1021 tcagtccaga gaccgaatag gcacaggagg acaagttgtg gactgcagtc gtttatttga
     1081 taacgattta ccagacggct gggaagaaag gagaaccgcc tctggaagaa tccagtatct
     1141 aaaccatata acaagaacta cgcaatggga gcgcccaaca cgaccggcat ccgaatattc
     1201 tagccctggc agacctctta gctgctttgt tgatgagaac actccaatta gtggaacaaa
     1261 tggtgcaaca tgtggacagt cttcagatcc caggctggca gagaggagag tcaggtcaca
     1321 acgacataga aattacatga gcagaacaca tttacatact cctccagacc taccagaagg
     1381 ctatgaacag aggacaacgc aacaaggcca ggtgtatttc ttacatacac agactggtgt
     1441 gagcacatgg catgatccaa gagtgcccag ggatcttagc aacatcaatt gtgaagagct
     1501 tggtccattg cctcctggat gggagatccg taatacggca acaggcagag tttatttcgt
     1561 tgaccataac aacagaacaa cacaatttac agatcctcgg ctgtctgcta acttgcattt
     1621 agttttaaat cggcagaacc aattgaaaga ccaacagcaa cagcaagtgg tatcgttatg
     1681 tcctgatgac acagaatgcc tgacagtccc aaggtacaag cgagacctgg ttcagaaact
     1741 aaaaattttg cggcaagaac tttcccaaca acagcctcag gcaggtcatt gccgcattga
     1801 ggtttccagg gaagagattt ttgaggaatc atatcgacag gtcatgaaaa tgagaccaaa
     1861 agatctctgg aagcgattaa tgataaaatt tcgtggagaa gaaggccttg actatggagg
     1921 cgttgccagg gaatggttgt atctcttgtc acatgaaatg ttgaatccat actatggcct
     1981 cttccagtat tcaagagatg atatttatac attgcagatc aatcctgatt ctgcagttaa
     2041 tccggaacat ttatcctatt tccactttgt tggacgaata atgggaatgg ctgtgtttca
     2101 tggacattat attgatggtg gtttcacatt gcctttttat aagcaattgc ttgggaagtc
     2161 aattaccttg gatgacatgg agttagtaga tccggatctt cacaacagtt tagtgtggat
     2221 acttgagaat gatattacag gtgttttgga ccataccttc tgtgttgaac ataatgcata
     2281 tggtgaaatt attcagcatg aacttaaacc aaatggcaaa agtatccctg ttaatgaaga
     2341 aaataaaaaa gaatatgtca ggctctatgt gaactggaga tttttacgag gcattgaggc
     2401 tcaattcttg gctctgcaga aaggatttaa tgaagtaatt ccacaacatc tgctgaagac
     2461 atttgatgag aaggagttag agctcattat ttgtggactt ggaaagatag atgttaatga
     2521 ctggaaggta aacacccggt taaaacactg tacaccagac agcaacattg tcaaatggtt
     2581 ctggaaagct gtggagtttt ttgatgaaga gcgacgagca agattgcttc agtttgtgac
     2641 aggatcctct cgagtgcctc tgcagggctt caaagcattg caaggtgctg caggcccgag
     2701 actctttacc atacaccaga ttgatgcctg cactaacaac ctgccgaaag cccacacttg
     2761 cttcaatcga atagacattc caccctatga aagctatgaa aagctatatg aaaagctgct
     2821 aacagccatt gaagaaacat gtggatttgc tgtggaatac ccctacgacg tgcctgacta
     2881 cgccggcggc ggtggttcag gcggtggcgg ttccggtggt gggggatcag tccaactggt
     2941 ggagtctggt ggcgctttgg tgcagccagg tggctctctg cgtttgtcct gtgccgcttc
     3001 tggcttccca gtgaaccgct attccatgcg ctggtatcgc caggctccag gcaaagagcg
     3061 tgagtgggta gccggtatgt ccagcgcggg tgatcgtagc tcctatgaag actccgtgaa
     3121 gggccgtttc accatcagcc gtgacgatgc ccgtaacacg gtgtatctgc aaatgaacag
     3181 cttgaaacct gaagatacgg ccgtgtatta ctgtaatgtg aacgtgggct tcgagtattg
     3241 gggccaaggc acccaggtca ccgtctccag ctaagatcct tgacttgcgg ccgcaactcc
     3301 cacctgcaac atgcgtgact gactgaggcc gcgactctag agtcgacctg catctaggcg
     3361 ccggaattag atctctcgag gttaacgaat tctaccgggt aggggaggcg cttttcccaa
     3421 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     3481 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     3541 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     3601 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     3661 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     3721 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     3781 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     3841 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     3901 gcagcccaag cttaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     3961 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     4021 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     4081 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     4141 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     4201 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     4261 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     4321 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     4381 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     4441 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     4501 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     4561 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     4621 caaggcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc
     4681 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     4741 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     4801 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4861 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4921 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4981 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     5041 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     5101 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     5161 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     5221 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     5281 atcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5341 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5401 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5461 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5521 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5581 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5641 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     5701 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5761 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5821 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5881 atcgataccg tcgaccaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5941 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6001 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6061 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6121 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     6181 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     6241 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     6301 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     6361 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     6421 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     6481 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     6541 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     6601 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     6661 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     6721 cgtcgaatgg tgcactctca gtacaatcta gctctgatgc cgcatagtta agccagcccc
     6781 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     6841 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     6901 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6961 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7021 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7081 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7141 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7201 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7261 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7321 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7381 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7441 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7501 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7561 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7621 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7681 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7741 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7801 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7861 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7921 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7981 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8041 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8101 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8161 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8221 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8281 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8341 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8401 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8461 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8521 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8581 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8641 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8701 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8761 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8821 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8881 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8941 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     9001 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9061 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9121 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9181 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9241 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9301 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     9361 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     9421 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     9481 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     9541 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     9601 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
     9661 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     9721 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     9781 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     9841 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     9901 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     9961 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
    10021 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
    10081 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
    10141 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
    10201 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
    10261 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
    10321 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
    10381 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
    10441 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
    10501 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
    10561 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
    10621 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
    10681 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
    10741 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
    10801 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
    10861 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
    10921 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
    10981 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    11041 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    11101 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    11161 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    11221 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    11281 caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11341 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
    11401 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    11461 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
    11521 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
    11581 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
    11641 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
    11701 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
    11761 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
    11821 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    11881 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    11941 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    12001 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
    12061 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
    12121 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
    12181 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
    12241 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
    12301 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
    12361 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
    12421 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    12481 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggtgatctt
    12541 cagacctgga cgatatatat gagggacaat tggagaagtg aattatataa atataaagta
    12601 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
    12661 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
    12721 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
    12781 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
    12841 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
    12901 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
    12961 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
    13021 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    13081 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    13141 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    13201 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    13261 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
    13321 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
    13381 gtgaacggat ctcgacggtc g
//
