LOCUS       V033464                11955 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033464
VERSION     V033464
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..1411
                     /label="NSMCE1(NM_145080)"
                     /note="NSMCE1(NM_145080)"
                     /gene="NSMCE1"
     CDS             1412..1438
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1484..1825
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        1951..2450
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             2471..3178
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3179..3235
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             3236..3835
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    3846..4434
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             4497..4730
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(4755..4773)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        5562..5666
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5667..6527
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6698..7286
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        7532..7861
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          8995..9060
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             9190..9210
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    9635..9769
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             9938..10571
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    10618..10743
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    11235..11468
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             11653..11697
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttcttaaggt gaagcagtga
      601 actccaagcc accatgcagg gcagcacaag gagaatgggc gtcatgactg atgtccaccg
      661 gcgcttcctc cagttgctga tgacccatgg cgtgctagag gaatgggacg tgaagcgctt
      721 gcagacgcac tgctacaagg tccatgaccg caatgccacc gtagataagt tggaggactt
      781 catcaacaac attaacagtg tcttggagtc cttgtatatt gagataaaga gaggagtcac
      841 ggaagatgat gggagaccca tttatgcgtt ggtgaatctt gctacaactt caatttccaa
      901 aatggctacg gattttgcag agaatgaact ggatttgttt agaaaggctc tggaactgat
      961 tattgactca gaaaccggct ttgcgtcttc cacaaacata ttgaacctgg ttgatcaact
     1021 taaaggcaag aagatgagga agaaggaagc ggagcaggtg ctgcagaagt ttgttcaaaa
     1081 caagtggctg attgagaagg aaggggagtt caccctgcac ggccgggcca tcctggagat
     1141 ggagcaatac atccgggaga cgtaccccga cgcggtgaag atctgcaata tctgtcacag
     1201 cctcctcatc cagggtcaaa gctgcgaaac ctgtgggatc aggatgcact taccctgcgt
     1261 ggccaagtac ttccagtcga atgctgaacc gcgctgcccc cactgcaacg actactggcc
     1321 ccacgagatc ccaaaagtct tcgaccctga gaaggagagg gagtctggtg tcttgaaatc
     1381 gaacaaaaag tccctgcggt ccaggcagca ttacccctac gacgtgcctg actacgccgg
     1441 cggcggtggt tcaggcggtg gcggttccgg tggtggggga tcagtccaac tggtggagtc
     1501 tggtggcgct ttggtgcagc caggtggctc tctgcgtttg tcctgtgccg cttctggctt
     1561 cccagtgaac cgctattcca tgcgctggta tcgccaggct ccaggcaaag agcgtgagtg
     1621 ggtagccggt atgtccagcg cgggtgatcg tagctcctat gaagactccg tgaagggccg
     1681 tttcaccatc agccgtgacg atgcccgtaa cacggtgtat ctgcaaatga acagcttgaa
     1741 acctgaagat acggccgtgt attactgtaa tgtgaacgtg ggcttcgagt attggggcca
     1801 aggcacccag gtcaccgtct ccagctaaga tccttgactt gcggccgcaa ctcccacctg
     1861 caacatgcgt gactgactga ggccgcgact ctagagtcga cctgcatcta ggcgccggaa
     1921 ttagatctct cgaggttaac gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     1981 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     2041 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     2101 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     2161 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     2221 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     2281 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     2341 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     2401 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     2461 caagcttacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     2521 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     2581 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     2641 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     2701 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     2761 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     2821 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     2881 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     2941 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     3001 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     3061 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     3121 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggc
     3181 aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatgac
     3241 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     3301 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     3361 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     3421 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     3481 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     3541 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     3601 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     3661 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     3721 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     3781 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgatctta
     3841 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3901 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3961 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4021 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4081 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4141 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4201 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     4261 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4321 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4381 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     4441 accgtcgacc aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4501 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     4561 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4621 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4681 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa
     4741 ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     4801 tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc
     4861 ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga
     4921 cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc
     4981 agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc
     5041 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     5101 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     5161 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
     5221 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     5281 atggtgcact ctcagtacaa tctagctctg atgccgcata gttaagccag ccccgacacc
     5341 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5401 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5461 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5521 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5581 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5641 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5701 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5761 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5821 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5881 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5941 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6001 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6061 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6121 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6181 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6241 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6301 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6361 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6421 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6481 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6541 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6601 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6661 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6721 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6781 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6841 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6901 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6961 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7021 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7081 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7141 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7201 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7261 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7321 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7381 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7441 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7501 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7561 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7621 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7681 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7741 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7801 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7861 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
     7921 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     7981 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     8041 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     8101 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
     8161 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
     8221 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
     8281 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     8341 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
     8401 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
     8461 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
     8521 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
     8581 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     8641 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     8701 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
     8761 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
     8821 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
     8881 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
     8941 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     9001 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     9061 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     9121 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     9181 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     9241 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     9301 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     9361 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     9421 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     9481 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
     9541 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     9601 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     9661 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9721 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     9781 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     9841 cctagtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
     9901 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
     9961 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
    10021 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
    10081 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
    10141 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
    10201 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
    10261 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
    10321 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
    10381 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    10441 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10501 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    10561 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
    10621 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
    10681 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
    10741 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
    10801 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
    10861 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
    10921 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
    10981 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
    11041 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggtga tcttcagacc
    11101 tggacgatat atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    11161 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    11221 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    11281 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    11341 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    11401 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    11461 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    11521 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    11581 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    11641 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    11701 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    11761 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    11821 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    11881 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
    11941 ggatctcgac ggtcg
//
