LOCUS       V030048                13362 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030048
VERSION     V030048
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2818
                     /label="MID2(NM_012216)"
                     /note="MID2(NM_012216)"
                     /gene="MID2"
     CDS             2819..2845
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2891..3232
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        3358..3857
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3878..4585
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4586..4642
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4643..5242
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5253..5841
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5904..6137
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(6162..6180)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6969..7073
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7074..7934
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8105..8693
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8939..9268
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          10402..10467
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10597..10617
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    11042..11176
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             11345..11978
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    12025..12150
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12642..12875
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             13060..13104
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctcgattg gttgcagtga
      601 actccaagcc accatgggtg aaagcccagc ctccgtggtt cttaatgcct caggaggact
      661 attttcacta aagatggaaa cactggagtc tgaattgacc tgtccaatct gcctagagtt
      721 gtttgaagac ccccttctgc tcccttgtgc tcacagcctc tgcttcagct gtgcccatcg
      781 cattttggta tcaagctgca gctctggtga atccattgaa cccattactg ctttccagtg
      841 tcctacctgc aggtatgtta tctcgctgaa ccaccggggc ctggatggcc tcaagaggaa
      901 tgtgactctg cagaacatta ttgatcgctt ccagaaggct tcagtcagtg ggcccaattc
      961 ccctagtgag agccgccggg aaaggactta caggcccacc actgccatgt ctagcgagcg
     1021 aattgcttgc caattctgtg agcaggaccc gccaagggat gcagtaaaaa catgcatcac
     1081 ctgtgaggtc tcctactgtg accgttgcct gcgggccacg caccccaaca agaaaccttt
     1141 caccagccac cgcctggtgg aaccagtgcc agacacacat cttcgaggga tcacctgcct
     1201 ggaccatgag aatgagaaag tgaacatgta ctgtgtatct gatgaccaat tgatctgtgc
     1261 cttatgcaaa ctggtgggtc gtcaccgaga ccatcaggtc gcatccctga atgatcgatt
     1321 tgagaaactc aagcaaactc tggagatgaa cctcaccaac ctggttaagc gcaacagcga
     1381 actagaaaat caaatggcca aactaataca gatctgccag caggttgagg tgaatactgc
     1441 tatgcatgag gcaaaactta tggaagaatg tgacgagttg gtagagatca tccagcagag
     1501 gaagcaaatg atcgctgtca aaatcaaaga gacaaaggtt atgaaactga gaaagttggc
     1561 acagcaggtt gctaattgcc gccagtgtct tgaacggtca acagtcctca tcaaccaagc
     1621 tgagcatatc ctgaaagaaa atgaccaggc acggtttcta cagtctgcaa aaaatattgc
     1681 tgagagggtc gctatggcaa ctgcatcttc tcaagttctg attccagaca tcaattttaa
     1741 tgatgccttt gaaaactttg ctttagattt ttccagagaa aagaaactgc tagaggggtt
     1801 agattattta acagccccaa acccaccatc tatccgagaa gaactctgta ctgcctccca
     1861 tgacaccatt acagtccact ggatctcgga tgatgagttc agcatcagct cctatgagct
     1921 tcagtacacc atattcactg gccaggctaa cttcatcagt aagtcatggt gtagttgggg
     1981 cctgtggcca gagataagga aatgtaagga agcagtaagc tgctcaagat tggccggggc
     2041 gccacgaggc ctgtataatt cagtagacag ctggatgatt gttcccaaca ttaaacagaa
     2101 ccattacaca gtgcatggac tccagagcgg gactcgctac atcttcatcg ttaaagccat
     2161 aaaccaagcc ggcagccgga acagtgaacc tacccgacta aaaacaaaca gccaaccctt
     2221 taaattggat cccaaaatga ctcacaagaa gttgaagatc tccaatgatg gattgcagat
     2281 ggagaaggat gaaagctctc taaagaagag ccacacccca gagaggttta gtggcacagg
     2341 gtgctatggg gcagcaggaa atatattcat tgacagtggc tgccactatt gggaggtggt
     2401 catgggttcc tcaacatggt atgcaattgg cattgcctac aaatcagctc caaagaatga
     2461 atggattggc aagaatgcct cctcatgggt cttctctcgc tgcaatagta acttcgtggt
     2521 gagacacaac aacaaggaaa tgctggtgga tgtgccccca cacctgaagc gtctgggtgt
     2581 cctcctggat tatgacaaca atatgctgtc tttctatgac ccagctaact ctctccatct
     2641 tcatactttt gatgtgacct tcattcttcc agtttgtcca acatttacaa tctggaacaa
     2701 atccctaatg atcctgtctg gcttgcctgc cccagatttt attgattacc ctgagcggca
     2761 ggaatgcaac tgcaggcctc aagaatcccc ttatgtttct gggatgaaaa cctgtcatta
     2821 cccctacgac gtgcctgact acgccggcgg cggtggttca ggcggtggcg gttccggtgg
     2881 tgggggatca gtccaactgg tggagtctgg tggcgctttg gtgcagccag gtggctctct
     2941 gcgtttgtcc tgtgccgctt ctggcttccc agtgaaccgc tattccatgc gctggtatcg
     3001 ccaggctcca ggcaaagagc gtgagtgggt agccggtatg tccagcgcgg gtgatcgtag
     3061 ctcctatgaa gactccgtga agggccgttt caccatcagc cgtgacgatg cccgtaacac
     3121 ggtgtatctg caaatgaaca gcttgaaacc tgaagatacg gccgtgtatt actgtaatgt
     3181 gaacgtgggc ttcgagtatt ggggccaagg cacccaggtc accgtctcca gctaagatcc
     3241 ttgacttgcg gccgcaactc ccacctgcaa catgcgtgac tgactgaggc cgcgactcta
     3301 gagtcgacct gcatctaggc gccggaatta gatctctcga ggttaacgaa ttctaccggg
     3361 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     3421 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     3481 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     3541 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     3601 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     3661 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     3721 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     3781 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     3841 catctccggg cctttcgacc tgcagcccaa gcttaccatg gtgagcaagg gcgaggagga
     3901 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     3961 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     4021 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     4081 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     4141 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     4201 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     4261 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     4321 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     4381 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     4441 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     4501 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     4561 cggcggcatg gacgagctgt acaaggcaac aaacttctct ctgctgaaac aagccggaga
     4621 tgtcgaagag aatcctggac cgatgaccga gtacaagccc acggtgcgcc tcgccacccg
     4681 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     4741 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     4801 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     4861 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     4921 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     4981 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     5041 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     5101 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     5161 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     5221 gacccgcaag cccggtgcct gatcttatcg ataatcaacc tctggattac aaaatttgtg
     5281 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5341 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5401 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5461 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5521 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5581 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5641 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5701 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5761 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5821 ccctttgggc cgcctccccg catcgatacc gtcgaccaag gcagctgtag atcttagcca
     5881 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     5941 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6001 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6061 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6121 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     6181 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     6241 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     6301 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     6361 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     6421 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     6481 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     6541 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     6601 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     6661 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct agctctgatg
     6721 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     6781 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     6841 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     6901 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     6961 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7141 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7201 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7261 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7321 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7381 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7441 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7501 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7561 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7621 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7681 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7921 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7981 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8041 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8161 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8221 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     8281 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8341 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8401 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8461 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8521 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8581 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8641 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8701 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8761 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8821 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8881 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     8941 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     9001 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     9061 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     9121 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9181 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9241 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     9301 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     9361 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     9421 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     9481 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     9541 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     9601 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     9661 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     9721 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     9781 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     9841 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     9901 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     9961 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
    10021 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
    10081 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
    10141 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
    10201 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
    10261 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
    10321 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
    10381 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
    10441 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
    10501 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
    10561 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
    10621 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
    10681 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
    10741 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
    10801 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
    10861 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
    10921 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
    10981 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    11041 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    11101 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    11161 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    11221 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt
    11281 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    11341 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
    11401 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
    11461 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
    11521 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
    11581 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
    11641 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
    11701 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
    11761 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
    11821 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
    11881 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
    11941 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
    12001 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
    12061 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
    12121 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
    12181 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
    12241 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
    12301 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
    12361 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
    12421 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
    12481 cagcaagcgg ccggtgatct tcagacctgg acgatatata tgagggacaa ttggagaagt
    12541 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
    12601 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
    12661 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
    12721 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
    12781 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
    12841 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
    12901 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
    12961 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
    13021 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
    13081 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
    13141 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
    13201 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
    13261 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
    13321 agagacagat ccattcgatt agtgaacgga tctcgacggt cg
//
